Molecular epidemiology of hepatitis A virus in patients in the ahwaz region of Iran

Nejati, Ahmad; Makvandi, Manoochehr; Samarbafzadeh, Alireza; Neisi, Niloofar; Moradzadegan, Hamid

doi:10.1002/jmv.23238

Cited by 11 publications

(8 citation statements)

References 15 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, worldwide, in most regions, there is co-circulation of IA and IB strains but the predominant strain usually accounts for more than 95% of HAV strains. Based on the recorded data, in Turkey and Middle East, 95% of HAV strains belong to genotype I subtype B [9,10], whereas in Europe the predominant strain is genotype I subtype A [11]. In Greece, the available data are in accordance with the other European countries [12].…”

Section: Discussionmentioning

confidence: 97%

Hepatitis A among refugees, asylum seekers and migrants living in hosting facilities, Greece, April to December 2016

et al. 2017

View full text Add to dashboard Cite

An increased number of hepatitis A cases among refugees, asylum seekers and migrants residing in hosting facilities in Greece were recorded between April and December 2016. In total, 177 laboratory-confirmed symptomatic cases were reported; of these, 149 (84%) occurred in hosting camps mostly among Syrian children under 15 years. All cases reported symptom onset after their entry into the country. Public health interventions focused on hygiene measures and vaccination.

show abstract

Section: Discussionmentioning

confidence: 97%

Hepatitis A among refugees, asylum seekers and migrants living in hosting facilities, Greece, April to December 2016

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Thus, based on the evaluation of 5UTR and VP12-A and the complete sequence of the HAV isolated from different regions of the world, it was confirmed that the isolated HAV strain from Ahvaz was a genotype IB. Nejati et al reported that the circulation of HAV genotype 1B among children with acute hepatitis A was dominant in Ahvaz city, Iran (17). The molecular epidemiology of HAV is limited in Iran and it is required to be investigated in different parts of Iran.…”

Section: Discussionmentioning

confidence: 99%

“…The PCR products were analyzed by electrophoresis through 2% agarose gel. The predicted PCR product size for VP1-2A was 234 bp (17).…”

Section: Nested Polymerase Chain Reaction For Vpv1-2amentioning

confidence: 99%

“…The subgenotype 1A is spreading in south and North America, Europe, Asia, and Africa, while IB is prevalent in Australia, the Middle East, and South Africa (12)(13)(14)(15)(16). The circulation of genotype 1B has been reported in Ahvaz city, Iran (17). Additionally, co-circulation of multiple genotypes has been documented in different regions around the world (18,19).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the Complete Sequence of HAV1B Isolated in Ahvaz City, Iran

Nejati

Teimori

Taherkhani

et al. 2019

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Hepatitis A virus (HAV) is a common cause of mild to acute hepatitis among children and adolescents, worldwide. Objectives: The aim of this study was to investigate the complete sequence of isolated HAV from a child with acute hepatitis. Methods: A serum sample was collected from a child with clinical sign and symptoms of acute hepatitis. Following RNA extraction, the genotype of virus was determined based on sequencing of VP1-2A region of the isolated HAV genome. The whole HAV genome was sequenced by the nested polymerase chain reaction (PCR) using specific primers. The size of the complete length of isolated HAV genome, 5UTR (Untranslated Region), and 3UTR were determined. The amino acid sequencing of single polyprotein, including VP1 and VP3 regions, were analyzed using the BioEdit software. To evaluate the isolated HAV genome, phylogenetic tree was conducted to analyze the complete HAV Genome, 5UTR, and VP1-2A regions of the isolated HAV. Simplot and RDP4 analyses were accomplished to confirm recombination in the genome of the sequestered HAV strain in Ahvaz city. Results: The sequestered HAV/Ahvaz/Iran/2015 was recorded with an accession number BankIt 2063303 MG 546669 in GenBank. The complete HAV sequence of the isolated HAV comprised of full length of 7239 nt, 5'UTR 619 nt, 3'UTR 21nt, and an open reading frame (ORF) encoding single polypeptide of 2200 amino acids (6600 nt). The whole sequences of isolated HAV strain Ahvaz/Iran/2015 showed 96% and 95% nucleotide identity with prototype strain HAV 1B isolated from Egypt (96%), South Africa (95%), and HM 175 (95%) strains, respectively. The complete amino acid sequence of the Vp1-2A region of the isolated HAV showed 100% identity with HM175 and 99% identity with isolated HAV from Egypt and South Africa. The detected HAV was identified as HAV genotype 1B. A mutation was observed in the amino acid sequences of VP3 region of the isolated HAV; this mutation showed substitution of isoleucine (I) with Arginine at position 433 amino acid sequence compared with the consensus sequences in amino acid of HM 175 strain. The results of RDP4 showed no evidence of recombination in the isolated strain HAV/Ahvaz/Iran/2015 genome. Conclusions: The isolated HAV was genotype1 B, comprised of full length of 7239 nt, 2200 amino acid. The complete amino acid sequence VP1 region of the isolated HAV showed 100% homology identity with HM175 and 99% with isolated HAV in Egypt, South Africa. A mutation was observed in the amino acid sequences of VP3 region of the isolated HAV, this mutation showed substitution of isoleucine (I) with Arginine at position 433 amino acid sequence compared with the consensus sequences amino acid of HM-175 strain. No evidence of recombination was observed in the isolated strain HAV/Ahvaz/Iran/2015 genome.

show abstract

“…In the present study, however, only two of the nine positive HAV samples (22.2%) were of the IA genotype and none were from genotype IIIA; the other seven HAV sequences belonged to genotype IB. Genotype IB has given rise to epidemics in Turkey, Spain, and Iran . One study reported detecting this genotype in 2.2% of clinical gastroenteritis patients in Korea during 2007–2008 .…”

Section: Discussionmentioning

confidence: 99%

Incidence and molecular characterization of hepatitis A viruses in Korean surface water between 2007 and 2010

Lee¹,

Kim²,

Nam³

et al. 2014

Microbiology and Immunology

View full text Add to dashboard Cite

Several bodies of surface water in Korea were surveyed for the presence of hepatitis A virus (HAV) between 2007 and 2010. Of 265 surface water samples, 9 (3.4%) were HAV-positive. HAVs were mainly detected in the summer (3/62, 4.8%) and spring (4/96, 4.2%) seasons. Comparing different water sources, the highest prevalence (6.6%) of positive samples was seen in lake water, four HAVpositive samples being from lakes. Comparing prevalence rates across the four representative Korean basin systems, no HAVs were found in the Han or Nakdong river basins. The highest HAV prevalence was found in samples from the Yeongsan river and other basins (6.3%); the Geum/Seom river was also found to have a high HAV prevalence (5.7%). HAVs from the nine positive samples were then sequenced and analyzed phylogenetically. Two of the HAVs belong to genotype IA and fall within the same cluster as HAVs 6-3(ASAN4) (EU049548), KANSAN-PS1 (EU049554), and ASAN-KM (EU049563), which were collected from the stools of patients with gastroenteritis in Korea. The seven other HAV nucleotide sequences belong to the genotype IB cluster. This is the first nationwide surveillance of HAV in major Korean water sources.

show abstract

Molecular epidemiology of hepatitis A virus in patients in the ahwaz region of Iran

Cited by 11 publications

References 15 publications

Hepatitis A among refugees, asylum seekers and migrants living in hosting facilities, Greece, April to December 2016

Hepatitis A among refugees, asylum seekers and migrants living in hosting facilities, Greece, April to December 2016

Investigation of the Complete Sequence of HAV1B Isolated in Ahvaz City, Iran

Incidence and molecular characterization of hepatitis A viruses in Korean surface water between 2007 and 2010

Contact Info

Product

Resources

About