Molecular epidemiology of dengue virus serotypes 2 and 3 isolated in Brazil from 1991 to 2008

Cruz, Ana Cecília Ribeiro; Galler, Ricardo; Silva, Eliana Vieira Pinto da; Silva, Mayra de Oliveira e; Carneiro, Adriana Ribeiro; Rosa, Elizabeth Salbé Travassos da; Vasconcelos, Helena Baldez; Sá, Eric Luiz Rodrigues de; Vasconcelos, Pedro Fernando da Costa

doi:10.5123/s2176-62232010000300004

Cited by 4 publications

(5 citation statements)

References 41 publications

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“…Studies have associated the occurrence of more severe forms of the disease to infections by DENV-2 [99,100]. Moreover, the higher genetic variability of this serotype may contribute to the disease severity [101].…”

Section: Discussionmentioning

confidence: 99%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

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Section: Discussionmentioning

confidence: 99%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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“…Phylogenetic analyses of DENV-2 showed that genotype III (Southeast Asian/American) was the only one that circulated over the past 19 years in Brazil, from 1991 to 2008 19 . Sequencing of samples collected in 2011 showed the presence of DENV-2 of the Asian/American genotype in Manaus 11 .…”

Section: Denv-2mentioning

confidence: 99%

Mosquito-transmitted viruses – the great Brazilian challenge

Mota

Terzian

Silva

et al. 2016

Brazilian Journal of Microbiology

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Arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. These viruses form a very diverse group, and in Brazil, arboviruses belonging to the families Flaviviridae and Togaviridae are predominant. Unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like Chikungunya virus and Zika virus.In Brazil, dengue is endemic, along with the presence of other arboviruses. The situation is complicated by the scarcity of diagnostic infrastructure and the absence of approved vaccines for these diseases. Disease control, thus, relies solely on vector control. Therefore, enhanced clinical knowledge and improved general awareness about these arboviruses are indispensable to tackle diagnostic inadequacies.

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“…Dengue transmission in Tocantins is endemic, with higher incidence between October and May (the rainy season). The circulation of all four virus serotypes was detected in the state, as well the co-circulation of two or more serotypes in many municipalities in previous years 14 15 Recently, Zika and chikungunya cases have also been reported.…”

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confidence: 94%

“…The circulation of all four virus serotypes was detected in the state, as well the co-circulation of two or more serotypes in many municipalities in previous years. 14 , 15 Recently, Zika and chikungunya cases have also been reported. This high incidence is exacerbated by the poor economic circumstances and the presence of environmental conditions favourable for mosquito development and arbovirus transmission in almost all municipalities.…”

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confidence: 99%

Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil

Sá

Rodovalho

Sousa

et al. 2019

Mem. Inst. Oswaldo Cruz

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BACKGROUND The longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. Tocantins is the newest state of the Brazilian Federation. Despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking.OBJECTIVES To verify the resistance status of Aedes aegypti from 10 populations distributed throughout the state connected by rivers and roads.METHODS Between 50 and 150 ovitraps were installed in house gardens within each municipality. Collection points were established based on the importance of the towns and on geographic aspects. Dose response bioassays were performed in accordance with World Health Organization guidelines. Molecular assays were conducted to detect kdr mutations, which are related to pyrethroid resistance.FINDINGS Of the 3,200 ovitrap paddles analysed, 25.8% contained eggs, with a total of 55,687 eggs collected. With the exception of Caseara, all evaluated populations were considered to be resistant to temephos. The data showed different levels of resistance to deltamethrin among the samples. Caseara and Guaraí showed the lowest RR95 values. On average, the NaVR1 kdr allele was most frequent (40.3%), followed by NaVS (38.1%), and NaVR2 (21.6%). Palmas, the capital of the state, had the highest frequency of kdr alleles (87.5%).MAIN CONCLUSIONS With the exception of Palmas, the towns with the highest indexes (ovitrap positivity, number and density of eggs), as well with high levels of resistance and kdr alleles were located along the BR-153 road, indicating that the flow of people and cargo can contribute to the dispersion of the vector and potentially resistance. This study contributes substantially to knowledge regarding the insecticide resistance profile of Tocantins mosquito populations; the data generated via the study could facilitate the judicious use of insecticides by vector control programs.

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Molecular epidemiology of dengue virus serotypes 2 and 3 isolated in Brazil from 1991 to 2008

Cited by 4 publications

References 41 publications

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Mosquito-transmitted viruses – the great Brazilian challenge

Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil

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