Molecular epidemiology of contagious bovine pleuropneumonia by multilocus sequence analysis of Mycoplasma mycoides subspecies mycoides biotype SC strains

Lorenzon, Sophie; Arzul, Isabelle; Peyraud, Armelle; Hendrikx, Pascal; Thiaucourt, François

doi:10.1016/s0378-1135(03)00043-9

Cited by 21 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This new MmmSC typing scheme proved more robust than the previous one developed in our laboratory [22]. No double bands were obtained after PCR amplification, which ensured that PCR products could be sent without any purification step.…”

Section: Epidemiological Analysismentioning

confidence: 85%

“…Analysis of PG1 sequences showed that many primers designed on a previous MLSA scheme [22] hybridised at multiple sites or targeted sequences that were duplicated in the PG1 genome. The initial validation of 19 selected loci in this study revealed that sequencing errors may remain in the published PG1 genome.…”

Section: Genomic Analysismentioning

confidence: 99%

“…The use of another insertion element, IS1634, also led to different Southern blot profiles [24,46] although the high copy number of this insertion sequence (N = 60), as compared to that of IS1296 (N = 28), gave rise to profiles that were difficult to analyse. The use of a technique based on sequencing multiple loci, designated multilocus sequence analysis (MLSA), allowed the identification of 15 different allelic profiles within a representative number of MmmSC strains (N = 48) of various origins [22]. In 2004 the whole genome sequence of MmmSC reference strain PG1 was published [48], opening new opportunities for the development of typing tools.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genotyping ofMycoplasma mycoidessubsp.mycoidesSC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

et al. 2008

View full text Add to dashboard Cite

-Mycoplasma mycoides subsp. mycoides SC (MmmSC) is the etiological agent of contagious bovine pleuropneumonia (CBPP). Although eradicated in most developed countries, the disease reappeared in Europe in the 1990s. This reappearance may have been caused either by importation from sub-Saharan Africa, where CBPP is still endemic, or by the reemergence of virulent strains in Europe, as suggested by earlier studies. A multilocus sequence analysis scheme has been developed to address this issue and, most importantly, to be able to monitor new epidemics. The alignment of the full genome sequence of the reference strain PG1 and the partial genome sequence of a pathogenic strain allowed the identification of polymorphic sites. Nineteen initial loci were selected within housekeeping genes, genes of unknown function and non coding sequences. The suitability of these loci for genotyping MmmSC strains was first tested on six strains of diverse geographic origin. The analyses showed that the published PG1 sequence contained a number of specific polymorphisms that were therefore of no use for molecular typing. Among the eight informative polymorphic loci finally selected, only one (ftsY) was positioned within a housekeeping gene. Three main groups and 31 different allelic profiles were identified among 51 strains and strain variants examined. Cluster analysis confirmed that European strains from the 1990s did not originate from Africa. It also showed a genetic link between a European strain isolated in 1967 and those found in southern Africa and Australia. This was in agreement with historical data showing that CBPP was introduced in these regions during colonisation in the 19th century.Mycoplasma mycoides / contagious bovine pleuropneumonia / multilocus sequence analysis / molecular epidemiology

show abstract

Section: Epidemiological Analysismentioning

confidence: 85%

Section: Genomic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotyping ofMycoplasma mycoidessubsp.mycoidesSC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

et al. 2008

View full text Add to dashboard Cite

show abstract

“…It is now known that East Africa shares a common genetic lineage of CBPP mycoplasmas (Lorenzon et al, 2003) and this suggests that regional CBPP strains share some characteristics. Thus, the model should be regionally applicable as far as the biological characteristics of the agent are concerned.…”

Section: Discussionmentioning

confidence: 99%

“…The introduction of CBPP into southern Africa from Europe in 1854 and its subsequent spread as far north as Angola are well documented (Windsor, 2000). These regional differences are borne out by recent molecular epidemiologic studies that have demonstrated three African lineages of CBPP (Lorenzon et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

A model of contagious bovine pleuropneumonia transmission dynamics in East Africa

Mariner¹,

McDermott

Heesterbeek

et al. 2006

Preventive Veterinary Medicine

View full text Add to dashboard Cite

The dynamics of contagious bovine pleuropneumonia (CBPP) transmission vary widely between livestock production systems. This paper describes the development of a homogeneous, stochastic, compartmental model for CBPP transmission in pastoral herds of East Africa. The model was built using parameter estimates based on data published in the literature and on observations of livestock owners obtained through participatory research. The basic reproduction number for CBPP in southern Sudan was estimated to range from 3.2 to 4.6. The homogeneous model indicates that the critical community size for the persistence of CBPP falls within the typical herd sizes for pastoral communities in East Africa suggesting that individual isolated herds are capable of maintaining infection indefinitely. Vaccination alone with currently available vaccines was unlikely to eradicate the disease. # 2005 Published by Elsevier B.V.

show abstract

A novel MLSA allelic profile ‘A15’ of Mycoplasma mycoides subsp mycoides in Niger

Yansambou¹,

Souley²,

Diallo

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

Molecular epidemiology of contagious bovine pleuropneumonia by multilocus sequence analysis of Mycoplasma mycoides subspecies mycoides biotype SC strains

Cited by 21 publications

References 32 publications

Genotyping ofMycoplasma mycoidessubsp.mycoidesSC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

Genotyping ofMycoplasma mycoidessubsp.mycoidesSC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

A model of contagious bovine pleuropneumonia transmission dynamics in East Africa

A novel MLSA allelic profile ‘A15’ of Mycoplasma mycoides subsp mycoides in Niger

Contact Info

Product

Resources

About