Molecular epidemiology of a large classical swine fever epidemic in the European Union in 1997–1998

Greiser‐Wilke, I.; Fritzemeier, J; Koenen, F.; Vanderhallen, H.; Rutili, D.; Mia, Gian Mario De; Romero, Luis; Rosell, Rosa; Sánchez‐Vizcaíno, José Manuel; Gabriel, Alberto San

doi:10.1016/s0378-1135(00)00253-4

Cited by 82 publications

(45 citation statements)

References 14 publications

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“…In the second, third and fourth experiment, three pigs each were inoculated with the moderately virulent strain Paderborn, with doses of respectively 10 2 TCID 50 (Paderborn low dose), 10 3.5 TCID 50 (Paderborn middle dose), and 10 5 TCID 50 (Paderborn high dose). This strain (genotype 2.1) was isolated in 1997 during the outbreak in the Paderborn area of Germany [10], and was used at cell passage level 5. In the fifth experiment, three pigs were inoculated with a dose of 100 LD 50 (50% lethal dose), which is approximately 10 2.5 TCID 50 , of the highly virulent strain Brescia.…”

Section: Viruses and Inoculation Of Animalsmentioning

confidence: 99%

“…Serum and EDTA-blood samples were collected from each pig at days 0, 3,5,7,10,12,14,17,19,21,24,28, and 35 p.i. Serum samples were stored at À70°C until testing for antibodies in the ELISA and NPLA (neutralization peroxidase-linked assay).…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…Oropharyngeal fluid and faeces were collected at days 0, 3,4,5,6,7,8,10,11,12,13,14,15,17,19,21,24,26,28,31, 33, and 35 p.i. Oropharyngeal fluid was obtained with a gauze tampon held by a 30 cm long forceps, which was scrubbed against the dorsal wall of the pharynx behind the soft palatum.…”

Section: Sampling Proceduresmentioning

confidence: 99%

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Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission

Weesendorp

Backer²,

Stegeman

et al. 2009

Vet. Res.

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-To improve the understanding of the dynamics and options for control of classical swine fever (CSF), more quantitative knowledge is needed on virus transmission. In this study, virus excretion and within-pen transmission of a strain of low, moderate and high virulence were quantified. Furthermore, the effect of inoculation dose on excretion and transmission were studied. The transmission was quantified using a stochastic susceptible-exposed-infectious-recovered (SEIR) model. Five transmission trials were conducted with ten pigs each. In each trial, three pigs were inoculated with the low virulent strain Zoelen, a low (10 2 TCID 50 ), middle (10 3.5 TCID 50 ), or high dose (10 5 TCID 50 ) of the moderately virulent strain Paderborn, or the highly virulent strain Brescia. The other seven pigs in each trial served as contact pigs. None of the pigs inoculated with the low dose of the Paderborn strain were infected. When it was assumed that the infectiousness of the pigs coincided with virus isolation positive oropharyngeal fluid and/or faeces, no significant differences in transmission rate b and basic reproduction ratio R 0 between the high inoculation dose of the Paderborn strain (b = 1.62/day, R 0 = 35.9) and the Brescia strain (b = 2.07/day, R 0 = 17.5) were observed. When the middle dose of the Paderborn strain was used for inoculation, the b (5.38/day) was not significantly higher than the Brescia strain or the high inoculation dose of the Paderborn strain, but the R 0 (148) was significantly higher. Infection with the Zoelen strain resulted in a significantly lower b and R 0 (b = 0/day, R 0 = 0) than the other strains.classical swine fever / transmission parameter / virus excretion / within-pen transmission

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Section: Viruses and Inoculation Of Animalsmentioning

confidence: 99%

Section: Sampling Proceduresmentioning

confidence: 99%

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Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission

Weesendorp

Backer²,

Stegeman

et al. 2009

Vet. Res.

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“…The sequence data generated was received as coloured chromatograph and text files. Phylogenetic tree for E2, NS5B and 5 0 UTR was constructed as described previously by Greiser-Wilke et al [7] by using the neighbor-joining method. The reference isolates for which all the three genomic region sequences are available in GenBank or in the nucleotide sequence database of European Union Reference Laboratory (EURL) originated from different parts of world (Supplementary Table 3) were selected and included in the multiple sequence alignment.…”

Section: Virus Isolation and Rt-pcrmentioning

confidence: 99%

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

et al. 2015

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The phylogenetic analysis of 11 CSFV isolates from Karnataka, India obtained during the year 2012-13 was undertaken to obtain the most reliable genetic typing of the CSFV isolates based on E2, NS5B and 5 0 UTR genomic regions. The study indicated that all the 11 CSFV isolates belonged to subgroup 2.2. The most reliable classification was obtained with sequence data from the NS5B region which separated all the isolates based on the history of outbreak and geographic origin. Analysis of full length E2 amino acid sequences revealed different genetic makeup of Indian 2.2 isolates compared to 2.2 isolates from different countries. The group 2.2 viruses are gradually spreading as confirmed by frequent detection/ isolation of group 2.2 viruses in the recent years and replacing the subgroup 1.1 viruses, which were hitherto predominantly involved in CSF outbreaks in India.Keywords Classical swine fever virus Á Phylogenetic analysis Á E2 Á NS5B and 5 0 UTR

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“…Vietnam isolate (CaTh05-1, AB252170) which belongs to subgroup 2.2 was considered closely related to Indian isolates having nucleotide percent identity ranging 94.6-98.7 among the subgroup 2.2 with 98.7% identity with (EU567079), Kaziranga (KF551933) and Assam (KJ645982) isolate and 98% with Lokhra (EF185782), Mathura (EU567081), Patna (EU567082), Supol (GU722590) and Darbhanga (KJ197334) isolate. The relationship between CSFV isolates circulating in India and its neighboring countries was determined by reconstructing the phylogenetic tree using 150 nt of 5 0 end of 5 0 UTR sequence of test isolate along with twenty-two reference sequences as described earlier [14]. Multiple sequence alignment was carried out using Clustal W algorithm [15] and Nucleotide divergence was calculated using MegAlign programme from LaserGene Biocomputing software Package (DNASTAR Inc.).…”

mentioning

confidence: 99%

Phylogenetic analysis of Classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates

Singh

Rajak

2017

VirusDis.

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Detection of Classical swine fever virus (CSFV) nucleic acid in archival formalin fixed tissue samples and their use for phylogenetic analysis was investigated. Ten samples were examined for the presence of CSFV nucleic acid by reverse transcriptase polymerase chain reaction (RT-PCR) amplification of 5 0 UTR and E2 gene. RT-PCR was found positive for 5 0 UTR fragment in eight samples while only one tissue samples showed amplification for E2 gene target fragment. For molecular epidemiology of the disease, 5 0 UTR PCR product of sample from Darbhanga (Bihar), was cloned and sequenced. The sequence was compared with the sequences available in database. The phylogenetic analysis reveals that the isolate belongs to subgroup 2.2 sharing 98.7% nucleotide identities with Vietnamese isolate (CaTh05-1, AB252170), indicating towards the possible origin of genogroup 2.2 CSFV isolates involved in the outbreak from Vietnam. From the study, it can be concluded that the tissue samples collected and stored in buffer formalin for years can be used to detect CSFV nucleic acid. Results are also suggestive of that the 5 0 UTR region of genome is more suitable target for RT-PCR based detection of CSFV in archival formalin fixed specimens. The study also indicates the potential of archival formalin fixed tissues for molecular epidemiology and genotyping of the CSF virus.

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Molecular epidemiology of a large classical swine fever epidemic in the European Union in 1997–1998

Cited by 82 publications

References 14 publications

Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission

Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

Phylogenetic analysis of Classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates

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