Molecular Epidemiology in Measles Control

Mulders, Mick N.

doi:10.1007/978-1-4615-1157-1_11

Cited by 6 publications

(6 citation statements)

References 112 publications

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“…Genotyping of MV is a useful tool for measuring the impact of measles vaccination, and has become an important component of measles surveillance [10,11]. Genetic characterization of wild‐type MVs combined with standard epidemiological methods helps to document chains of transmission and to illustrate the progress towards elimination by differentiating between indigenous and imported viruses [12–14].…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of wild-type measles viruses, circulating in the Russian Federation during the implementation of the National Measles Elimination Program, 2003–2007

Shulga

Rota

Kremer

et al. 2009

Clinical Microbiology and Infection

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Genetic characterization of wild-type measles viruses (MVs) is an important component of laboratory surveillance of measles. In this study, a phylogenetic analysis was performed of the nucleoprotein gene sequences of 228 MVs isolated in the Russian Federation between 2003 and 2007. Five genotypes, D4, D5, D6, D8, and H1, were detected. From 1999 through the first 6 months of 2003, the most prevalent genotype in the European part of Russia was D4. All genotype D4-type viruses were closely related to each other (with overall sequence diversity of

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Section: Introductionmentioning

confidence: 99%

Genetic variability of wild-type measles viruses, circulating in the Russian Federation during the implementation of the National Measles Elimination Program, 2003–2007

Shulga

Rota

Kremer

et al. 2009

Clinical Microbiology and Infection

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“…In Africa, indigenous measles virus (MV) genotypes seem to have a distinct geographic distribution ( 8 , 9 ). In the central and western parts of sub-Saharan Africa, mainly clade B viruses have been identified ( 10 – 17 ).…”

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confidence: 99%

“…Molecular epidemiology has proven to be a major component of measles surveillance because it enables the effect of accelerated measles control activities to be assessed and the elimination of endemic virus strains to be documented. In Africa, indigenous measles virus (MV) genotypes seem to have a distinct geographic distribution ( 8 , 9 ). In the central and western parts of sub-Saharan Africa, mainly clade B viruses have been identified ( 10 – 17 ).…”

mentioning

confidence: 99%

Measles Virus Strain Diversity, Nigeria and Democratic Republic of the Congo

Kremer¹,

Nkwembe²,

Oyefolu³

et al. 2010

Emerg. Infect. Dis.

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“…Integration of routine measles vaccination into the expanded programme of immunization (EPI) has dramatically reduced measles morbidity and mortality worldwide [Henao-Restrepo et al, 2003]. In America and a number of other countries, elimination of indigenous measles virus (MV, family: paramyxoviridae, genus: morbillivirus) strains was demonstrated by molecular epidemiology [Muller and Mulders, 2002;CDC, 2004a]. Molecular epidemiology has proven useful to distinguish between endemic and imported MV strains; therefore genotyping becomes increasingly important as countries move toward measles elimination [Hanses et al, 2000;Muller and Mulders, 2002;Rota et al, 2002].…”

Section: Introductionmentioning

confidence: 99%

“…In America and a number of other countries, elimination of indigenous measles virus (MV, family: paramyxoviridae, genus: morbillivirus) strains was demonstrated by molecular epidemiology [Muller and Mulders, 2002;CDC, 2004a]. Molecular epidemiology has proven useful to distinguish between endemic and imported MV strains; therefore genotyping becomes increasingly important as countries move toward measles elimination [Hanses et al, 2000;Muller and Mulders, 2002;Rota et al, 2002]. Nevertheless, measles is still endemic in many regions in Africa and Asia, where reinforced vaccination strategies are critical for further progress of measles control [CDC, 2003[CDC, , 2004bKremer and Muller, 2003;Otten et al, 2005].…”

Section: Introductionmentioning

confidence: 99%

Genotyping of recent measles virus strains from Russia and Vietnam by nucleotide‐specific multiplex PCR

Kremer

Nguyen

Shulga

et al. 2007

Journal of Medical Virology

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The nucleoprotein genes of 49 measles virus (MV) strains circulating in Russia between 2000 and 2006 and in Vietnam in 2003 were analyzed by genotype-specific PCR and the results were compared with their sequences. The sequences revealed the presence of genotypes H1 and H2 in the center (Nha Trang) and the north (Hanoi) of Vietnam, respectively. The relative diversity of the H2 strains suggested an endemic circulation of these viruses in the capital. In contrast genotype H1 strains from Nha Trang were homogenous genetically, which may indicate a recent importation. The strains obtained from 12 different regions of the Russian Federation were assigned to the genotypes H1, D4, and D6. Most strains (81.4%) were correctly genotyped by a multiplex PCR method which was sensitive to genotype-specific mutations [Kremer et al. (2004): J Clin Microbiol 42: 3017-3022]. Ambiguous or negative results for some clade H and genotype D6 strains were due to point mutations in the type-specific primer binding sites. After exchanging a single nucleotide in both the clade H- and the genotype D6-specific primers, all strains were assigned correctly to their genotype. A simplified procedure for use in Vietnam was developed to distinguish directly between genotypes H1 and H2 and any non-H genotype. These results demonstrate that our multiplex PCR method can be adapted easily to new sequence variants or specific epidemiological situations, and thus be very useful for rapid genotyping of large number of samples even in laboratories which do not have sequencing facilities.

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Molecular Epidemiology in Measles Control

Cited by 6 publications

References 112 publications

Genetic variability of wild-type measles viruses, circulating in the Russian Federation during the implementation of the National Measles Elimination Program, 2003–2007

Genetic variability of wild-type measles viruses, circulating in the Russian Federation during the implementation of the National Measles Elimination Program, 2003–2007

Measles Virus Strain Diversity, Nigeria and Democratic Republic of the Congo

Genotyping of recent measles virus strains from Russia and Vietnam by nucleotide‐specific multiplex PCR

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