Molecular epidemiology and subtyping of Blastocystis sp. and its subtypes in celiac patients; a case control study

Jevinani, Sara Soleimani; Rahimi, Hanieh Mohammad; Asri, Nastaran; Rostami-Nejad, Mohammad; Ahmadipour, Shokoufeh; Mirjalali, Hamed

doi:10.1016/j.micpath.2023.106086

Cited by 5 publications

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of course, molecular researches indicate that zoonotic transmission can occur (16). data around subtypes of B. hominis in Iran is limited to some researches (1,(17)(18)(19)(20)(21)(22)(23) and few study has surveyed the relation between IBS and B. hominis (19,24). Researches based on the comparison of rRNA gene of small nuclear subunit shows that B. hominis has a wide molecular diversity.…”

Section: Introductionmentioning

confidence: 99%

Irritable bowel syndrome associated with B. hominis or without relationship of it? A case control study and minireview

Maghsood,

Kayedimajd,

Motavallihaghi

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Blastocystis hominis (B. hominis) is a protozoan parasite that is found worldwide distribution. some studies have suggested a link between B. hominis and the development of irritable bowel syndrome (IBS). The objective of this study was to determine the prevalence of B. hominis in patients with IBS compared to healthy individuals. Material and methods: A total of 65 stool samples from patients with IBS and 65 samples from healthy individuals in northern Iran were examined, the samples were tested using various methods including direct smear, formalin ether sedimentation and culture to detect the presence of B. hominis. Additionally, polymerase chain reaction (PCR) was performed on all culture-positive isolates to confirm the results and identify the genotype. Results: B. hominis was detected in 15.38% of IBS patients and 9.2% of the healthy group. The culture in RPMI1640 was found to be better than the formalin ether and direct smear methods. Positive samples were confirmed using the molecular method. No significant difference was observed in the order of B. hominisinfection between the two groups. Conclusions: The results of our study indicate that no significant difference was observed in the order of B. hominisinfection between IBS patients and healthy groups. Therefore, firthet, study is necessary to determine the potential pathogenic effects of this parasite and its role in causing IBS.

show abstract