Molecular epidemiology and spatial distribution of <i>Selenomonas</i> spp. in subgingival biofilms

Drescher, Julia; Schlafer, Sebastian; Schaudinn, Christoph; Riep, Birgit; Neumann, Konrad; Friedmann, Anton; Petrich, Annett; Göbel, Ulf B.; Moter, Annette

doi:10.1111/j.1600-0722.2010.00765.x

Cited by 38 publications

(45 citation statements)

References 40 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this information would need to be confirmed by future studies evaluating the prevalence and levels of this microorganism in other populations. The other exceptions of microorganisms associated with periodontitis in the present review that may inhabit extraoral environments are S. sputigena, T. medium, and species from the Liu et al, 2012;Drescher et al, 2010Drescher et al, 2010 Selenomonas sputigena HOT 151 Kumar et al, 2003;Mayanagi et al, 2004;Griffen et al, 2012;Abusleme et al, 2013Gonçalves et al, 2012 Kumar et al, 2003;Griffen et al, 2012;Abusleme et al, 2013;Riep et al, 2009 Treponema medium HOT 667 Asai et al, 2002;Kumar et al, 2003;Mayanagi et al, 2004;Griffen et al, 2012;Abusleme et al, 2013Treponema vincentii HOT 029 Willis et al, 1999Asai et al, 2002;Griffen et al, 2012 Treponema sp. oral Brinig et al, 2003;Ouverney et al, 2003;Liu et al, 2012 Synergistetes and Candidatus Saccharibacteria phyla.…”

Section: Discussionmentioning

confidence: 75%

Newly Identified Pathogens Associated with Periodontitis

et al. 2014

View full text Add to dashboard Cite

There is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. Nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. However, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. The aim of this systematic review was to determine the current weight of evidence for newly identified periodontal pathogens based on the results of "association" studies. This review was conducted and reported in accordance with the PRISMA statement. The MEDLINE, EMBASE, and Cochrane databases were searched up to September 2013 for studies (1) comparing microbial data of subgingival plaque samples collected from subjects with periodontitis and periodontal health and (2) evaluating at least 1 microorganism other than the already-known periodontal pathogens. From 1,450 papers identified, 41 studies were eligible. The data were extracted and registered in predefined piloted forms. The results suggested that there is moderate evidence in the literature to support the association of 17 species or phylotypes from the phyla Bacteroidetes, Candidatus Saccharibacteria, Firmicutes, Proteobacteria, Spirochaetes, and Synergistetes. The phylum Candidatus Saccharibacteria and the Archaea domain also seem to have an association with disease. These data point out the importance of previously unidentified species in the etiology of periodontitis and might guide future investigations on the actual role of these suspected new pathogens in the onset and progression of this infection.

show abstract

Section: Discussionmentioning

confidence: 75%

Newly Identified Pathogens Associated with Periodontitis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Fluorescence in situ hybridization (FISH) targeting rRNA (11,12) can be used to identify nearly any microbe, but because of technical limitations, it is generally used to differentiate only two or three microbial types simultaneously. The resulting images reveal distinctive distributions of individual organisms (13)(14)(15) but not the overall structure of the community. However, fluorescence spectral imaging allows the differentiation of many fluorophores and creates an opportunity to take a systems-level view of the spatial structure of the microbiota (16), simultaneously imaging and identifying all members of a complex microbial consortium.…”

Section: Significancementioning

confidence: 99%

Biogeography of a human oral microbiome at the micron scale

Welch

Rossetti

Rieken

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

666

528

View full text Add to dashboard Cite

The spatial organization of complex natural microbiomes is critical to understanding the interactions of the individual taxa that comprise a community. Although the revolution in DNA sequencing has provided an abundance of genomic-level information, the biogeography of microbiomes is almost entirely uncharted at the micron scale. Using spectral imaging fluorescence in situ hybridization as guided by metagenomic sequence analysis, we have discovered a distinctive, multigenus consortium in the microbiome of supragingival dental plaque. The consortium consists of a radially arranged, nine-taxon structure organized around cells of filamentous corynebacteria. The consortium ranges in size from a few tens to a few hundreds of microns in radius and is spatially differentiated. Within the structure, individual taxa are localized at the micron scale in ways suggestive of their functional niche in the consortium. For example, anaerobic taxa tend to be in the interior, whereas facultative or obligate aerobes tend to be at the periphery of the consortium. Consumers and producers of certain metabolites, such as lactate, tend to be near each other. Based on our observations and the literature, we propose a model for plaque microbiome development and maintenance consistent with known metabolic, adherence, and environmental considerations. The consortium illustrates how complex structural organization can emerge from the micron-scale interactions of its constituent organisms. The understanding that plaque community organization is an emergent phenomenon offers a perspective that is general in nature and applicable to other microbiomes.biofilm | imaging | microscopy | microbial ecology B iogeography-the study of the distribution of organisms across the globe-seeks to recognize patterns in the spatial distribution of organisms and discover the forces that underlie those patterns. Bacteria are micron-sized, and many of the forces and factors that underlie their distributional patterns operate at micron scales and are qualitatively different from the large-scale factors, such as climate, that drive traditional biogeography. To frame the analysis of microbial distribution patterns at the scale that microbes themselves experience, we introduce the concept of micron-scale biogeography: the study of the distribution of microbes relative to micron-scale features of their environment. These features include the host or inanimate surfaces on which the microbes reside as well as local gradients of nutrients and oxygen. Key components of the micron-scale environment, particularly in biofilms and other densely populated habitats, are the microbes themselves, serving as substrates for attachment of other microbes, creating spatial structure, and acting as point sources for diffusible metabolites.Micron-scale biogeography is critical to understanding the physiology and ecology of the community as well as its systems biology and its effects on human health and disease. Close proximity or physical contact between two microbes can substantially a...

show abstract

“…Oral members of the bacterial genus Selenomonas have repeatedly been associated with periodontal disease and recent studies support the association of Selenomonas with the etiology of periodontitis, based on the detection of Selenomonas species at significant levels in subgingival biofilm samples of subjects with both chronic (17) and aggressive (18) periodontitis. It appears that Selenomonas species contribute considerably to the structural organization of multispecies oral biofilms ("dental plaque"), which is the condition under which periodontitis develops (19). Periodontitis continues to be the most frequently occurring inflammatory disease world-wide; in its chronic form, it is the major cause of tooth loss and can also impact systemic health (20), underlining the urgent need for therapeutic interference.…”

Section: Introductionmentioning

confidence: 99%