Molecular Epidemiology and Public Health Relevance of<i>Campylobacter</i>Isolated from Dairy Cattle and European Starlings in Ohio, USA

Sanad, Yasser M.; Closs, Gary; Kumar, Anand; LeJeune, Jeffrey T.; Rajashekara, Gireesh

doi:10.1089/fpd.2012.1293

Cited by 39 publications

(37 citation statements)

References 55 publications

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“…Surprisingly, Campylobacter species are abundant in the large intestine, especially in the cecum. Although this genus is usually known to comprise pathogenic bacteria, some species isolated from cattle and starlings show a high resistance to multiple antimicrobial drugs, including ciprofloxacin , gentamicin , and erythromycin (Sanad et al 2013). Finally, Fibrobacter , Dialister , and Succiniclasticum were found more abundant in the stomach than in the small and large intestine.…”

Section: Discussionmentioning

confidence: 99%

Microbial community compositions in the gastrointestinal tract of Chinese Mongolian sheep using Illumina MiSeq sequencing revealed high microbial diversity

Zeng

et al. 2017

AMB Expr

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Chinese Mongolian sheep are an important ruminant raised for wool and meat production. However, little is known about the microbiota of the gastrointestinal tract (GIT) of Chinese Mongolian sheep. To increase our understanding of the microbial community composition in the GIT of Chinese Mongolian sheep, microbiota of five sheep is investigate for the first time using the Illumina MiSeq platform. High microbial diversity was obtained from the GIT, and the microbiota exhibited a higher biodiversity in the stomach and large intestine than in the small intestine. Firmicutes (44.62%), Bacteroidetes (38.49%), and Proteobacteria (4.11%) were the three most abundant phyla present in the GIT of the sheep. The present study also revealed the core genera of Prevotella, Bacteroides, Ruminococcus, Oscillospira, Treponema, and Desulfovibrio in the GIT. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States indicated that the metabolic pathway related to carbohydrate metabolism was the richest in the sheep GIT. In addition, a series of metabolic pathways related to plant secondary metabolism was most abundant in the stomach and large intestine than in the small intestine. Overall, the present study provides insight into the microbial community composition in GIT of the Chinese Mongolian sheep which is highly diverse and needs to be studied further to exploit the complex interactions with the host.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0378-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Microbial community compositions in the gastrointestinal tract of Chinese Mongolian sheep using Illumina MiSeq sequencing revealed high microbial diversity

Zeng

et al. 2017

AMB Expr

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“…A total of 98 isolates (23 C. jejuni and 75 C. coli) representing different ages, farms, and flaA clusters were tested for antimicrobial susceptibility using Sensititre Campy plates (TREK Diagnostic Systems Inc., USA) as previously described (Sanad et al, 2011(Sanad et al, , 2013. Minimum inhibitory concentrations (MICs) were determined based on Clinical and Laboratory Standards Institute (CLSI, 2012) breakpoint interpretative criteria for Campylobacteraceae.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Phenotypic and Genotypic Diversity of ThermophilicCampylobacterspp. in Commercial Turkey Flocks: A Longitudinal Study

Kashoma

Kumar²,

Sanad³

et al. 2014

Foodborne Pathogens and Disease

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Poultry are recognized as a main reservoir of Campylobacter spp. However, longitudinal studies investigating the persistence of Campylobacter on commercial meat turkeys are rare. The objectives of this study were to determine the prevalence, antimicrobial susceptibility, and persistence of genotypically related strains of Campylobacter spp. recovered from three commercial turkey farms in Ohio belonging to a single producer. Eight hundred ten samples were collected from birds aged 1 week to slaughter, consisting of 750 fecal droppings and 60 ceca at slaughter. Overall Campylobacter prevalence was 55.9%. Multiplex polymerase chain reaction (PCR) confirmed 72.3% of all isolates as C. coli, 5.3% as C. jejuni, 10.6% as both, and 11.9% as other Campylobacter spp. PCR restriction fragment length polymorphism of the flaA gene subtyping detected 70 types-62 for C. coli and 8 for C. jejuni isolates-with most (80%) of flaA-types constituting farm homogeneous groups. Multilocus sequence typing of 99 selected Campylobacter isolates resulted in 23 sequence types (STs), consisting of 8 STs for C. jejuni and 15 STs for C. coli isolates. Six novel STs-four for C. jejuni and two-for C. coli, were detected. In a subset of isolates (n = 98) tested for antimicrobial resistance, the most common resistance was to tetracycline (95%), followed by azithromycin (43%), while 42% and 18% of the isolates were resistant to ciprofloxacin and erythromycin, respectively. All isolates were susceptible to florfenicol. C. coli isolates displayed a higher proportion of resistance than C. jejuni to most antimicrobials. This study highlights the high prevalence, genotypic diversity, and antimicrobial resistance of Campylobacter spp. in commercial turkey from farm to slaughter.

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“…For example, studies have revealed a range of Campylobacter prevalence (up to 70%) in cattle. 31,[74][75][76] Furthermore, although the prevalence of Campylobacter in cattle carcasses and milk samples can be relatively low in comparison to other sources, studies have shown that approximately 15% of beef carcasses/meat can be contaminated with these pathogens. 38,92 In addition, a Campylobacter prevalence of 41.7%, 10.2%, and 4.6% had been previously reported in raw bulk tank milk in Northern Italy, Pakistan, and Poland, respectively, while 6.25% of raw cow milk samples from retail stores were contaminated with Campylobacter in Iran.…”

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Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Kashoma

Kassem

John

et al. 2016

Microbial Drug Resistance

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Campylobacter species are commonly transmitted to humans through consumption of contaminated foods such as milk and meat. The aim of this study was to investigate the prevalence, antimicrobial resistance, and genetic determinants of resistance of Campylobacter isolated from raw milk and beef carcasses in Tanzania. The antimicrobial resistance genes tested included bla OXA-61 (ampicillin), aph-3-1 (aminoglycoside), tet(O) (tetracycline), and cmeB (multi-drug efflux pump). The prevalence of Campylobacter was 9.5% in beef carcasses and 13.4% in raw milk, respectively. Using multiplex-polymerase chain reaction (PCR), we identified 58.1% of the isolates as Campylobacter jejuni, 30.7% as Campylobacter coli, and 9.7% as other Campylobacter spp. One isolate (1.6%) was positive for both C. jejuni and C. coli specific PCR. Antimicrobial susceptibility testing using the disk diffusion assay and the broth microdilution method showed resistance to: ampicillin (63% and 94.1%), ciprofloxacin (9.3% and 11.8%), erythromycin (53.7% and 70.6%), gentamicin (0% and 15.7%), streptomycin (35.2% and 84.3%), and tetracycline (18.5% and 17.7%), respectively. Resistance to azithromycin (42.6%), nalidixic acid (64.8%), and chloramphenicol (13%) was determined using the disk diffusion assay only, while resistance to tylosin (90.2%) was quantified using the broth microdilution method. The bla OXA-61 (52.6% and 28.1%), cmeB (26.3% and 31.3%), tet(O) (26.3% and 31.3%), and aph-3-1 (5.3% and 3.0%) were detected in C. coli and C. jejuni. These findings highlight the extent of antimicrobial resistance in Campylobacter occurring in important foods in Tanzania. The potential risks to consumers emphasize the need for adequate control approaches, including the prudent use of antimicrobials to minimize the spread of antimicrobial-resistant Campylobacter.

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Molecular Epidemiology and Public Health Relevance ofCampylobacterIsolated from Dairy Cattle and European Starlings in Ohio, USA

Cited by 39 publications

References 55 publications

Microbial community compositions in the gastrointestinal tract of Chinese Mongolian sheep using Illumina MiSeq sequencing revealed high microbial diversity

Microbial community compositions in the gastrointestinal tract of Chinese Mongolian sheep using Illumina MiSeq sequencing revealed high microbial diversity

Phenotypic and Genotypic Diversity of ThermophilicCampylobacterspp. in Commercial Turkey Flocks: A Longitudinal Study

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

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