Molecular epidemiological survey of pathogenic trypanosomes in naturally infected cattle in northern Côte d’ivoire

Ekra, Jean-Yves; N’Goran, Edouard K.; Mboera, Leonard E.G.; Gragnon, Biégo Guillaume; Assovié, Koco Rita Nadège; Mafie, Eliakunda Michael

doi:10.3347/phd.22170

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…In this study, we noted more T. vivax sequences than T. congolense sequences, which was also noted by other researchers [ 16 , 17 , 30 ]. T vivax and T. theileri were present in mechanical vectors, notably Tabanidae .…”

Section: Discussionsupporting

confidence: 90%

“…The criteria for selecting farms and animals for blood sampling were the same as those described in our previous study [ 16 ]. Blood samples were collected from cattle in the field in a period from August to November 2021.…”

Section: Methodsmentioning

confidence: 99%

“…In Côte d’Ivoire, trypanosome diagnostic studies using the PCR technique have been exclusively carried out with microsatellite primers [ 15 , 16 , 17 , 18 ]. The primers used are specific to the T. vivax , T brucei and T. congolense savannah and forest species.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

Ekra,

Mafie,

N’Goran

et al. 2024

Pathogens

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The potential danger to livestock from African animal trypanosomiasis is well known. However, the trypanosome species circulating in cattle and their genetics are poorly understood. After different alignments according to three regions (ITS1, gGAPDH and rRNA gene) of the trypanosome genome, phylogenetic analyses were used to show the genetic diversity of the different species that were circulating in the cattle in three regions (Bagoue, Poro and Tchologo) of Côte d’Ivoire. These analyses were performed by alignment of ITS1; by alignment of partial 18S, ITS1, 5.8S, ITS2 and partial 28S rRNA genes; and by alignment of gGAPDH gene with sequences of Trypanosomes found in GenBank. Three species were identified (T. vivax, T. theileri and T. congolense) in the cattle in the three northern regions of Côte d’Ivoire. T. vivax and T. theileri were the most abundant species in the present study. Contrary to the other primers used in this study, the ITS1 primers were not able to amplify T. theileri. We observed mixed infections between T. theileri and the other two species identified (T. vivax and T. congolense). As far as primers are concerned, in some cases, rRNA was able to identify the same species of trypanosomes that the ITS1 and gGAPDH primers were able to identify. Two main distinct groups of T. theileri complex were identified. The T. congolense and T. vivax strains were close to African strains, such as those from Kenya, Nigeria and Cameroon, unlike the T. theileri strain. Three trypanosome species (T. vivax, T. theileri and T. congolense) circulate in cattle in the Savannah district of Côte d’Ivoire. The genetic diversity of the trypanosome species encountered in this study cannot be classified as intraspecies according to geographical area and breed of cattle they infect.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

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Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

Ekra,

Mafie,

N’Goran

et al. 2024

Pathogens

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Transient Exposure of Humans to Animal Trypanosomes in Communities Highly Exposed to Tsetse Fly Bite

Addo,

Tweneboah,

Addison

et al. 2024

Preprint

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Case reports of some Trypanosomes (Trypanosoma congolense, Trypanosoma evansi, Trypanosoma brucei brucei, and Trypanosoma vivax) thought to be natural parasites only to animals have been reported to cause “atypical human trypanosomiasis” (a-HT). This study therefore aimed to provide molecular-based evidence of the circulation of animal trypanosome ITS1 DNA in asymptomatic Ghanaian individuals. A cross-sectional community-based study design was used to collect venous blood and urine samples from 240 human participants. PCR assays were performed targeting Trypanosoma Internal Transcribed Spacer 1 (ITS1) and human apolipoprotein genes. Amplicons were purified and subcloned. Transformed cells were confirmed by PCR. Positive clones were cultured overnight, purified and sequenced. The overall rate of positive tests for trypanosome ITS1 DNA detection was 15.8% (38/240). The highest percentage of trypanosome ITS1 DNA detected (19.2%) was recorded for Zorh, followed by Nkatenkwan (17.9%). Trypanosome parasites found in this study area were Trypanozoon, T. congolense, T. vivaxand T. simiae. There were no mutations in codons 142 or 266 of the ApoL-1 gene in the trypanosome ITS1 DNA-positive subjects. There is a high rate of trypanosome ITS1 DNA detection among humans in the study area. This underscores the need to investigate the impact of animal parasites on human health.

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Molecular epidemiological survey of pathogenic trypanosomes in naturally infected cattle in northern Côte d’ivoire

Cited by 2 publications

References 21 publications

Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

Transient Exposure of Humans to Animal Trypanosomes in Communities Highly Exposed to Tsetse Fly Bite

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