Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon Island, the Philippines

Ochirkhuu, Nyamsuren; Konnai, Satoru; Mingala, Claro N.; Okagawa, Tomohiro; Villanueva, Marvin A.; Pilapil, Flor Marie Immanuelle R.; Shiro, Motoo; Ohashi, Kazuhiko

doi:10.1016/j.vetpar.2015.05.019

Cited by 23 publications

(16 citation statements)

References 21 publications

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“…in blood samples with an almost 100% detection rate implies widespread occurrence in dairy cattle from the five provinces and agrees with the results of previous studies [17,18]. Similarly, a high detection rate was reported in previous studies in the same provinces [16,18]. The high detection rate could be attributed to the prevalence of Rhipicephalus (Boophilus) microplus infestation in the study area.…”

Section: Discussionsupporting

confidence: 91%

“…CALABARZON in southern Luzon is reported to be among the regions with the highest cattle populations and contributes greatly to the country's milk production [19]. There are also previous studies from other Philippine islands such as Cebu [15,16,19], Negros, and Panay [17], which all together showed the endemicity of bovine anaplasmosis. Most of those previous studies only reported detection of A. marginale.…”

Section: Discussionmentioning

confidence: 85%

“…Previous studies have shown the prevalence of Anaplasma spp. in several provinces of Luzon, including the current area of study [16][17][18]. CALABARZON in southern Luzon is reported to be among the regions with the highest cattle populations and contributes greatly to the country's milk production [19].…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of Anaplasma spp. in blood and milk of dairy cattle in the Philippines

Cruz¹,

Galay²,

Sandalo³

et al. 2019

Turk J Vet Anim Sci

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Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasma species, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by nested PCR for Anaplasma spp. Selected amplicons were purified and subjected to sequence analysis. It was found that 97 (98.97%) blood samples and 6 (6.12%) milk samples were positive for Anaplasma. Sequence analysis revealed that the positive amplicons from milk samples and their corresponding blood samples shared a high identity (98%-100%) with reported Anaplasma marginale isolates. To the authors' knowledge, this study provides the first molecular evidence of the presence of A. marginale in milk from dairy cattle under field conditions in the Philippines.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Molecular detection of Anaplasma spp. in blood and milk of dairy cattle in the Philippines

Cruz¹,

Galay²,

Sandalo³

et al. 2019

Turk J Vet Anim Sci

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show abstract

“…Previous studies in neighboring provinces like Batangas, Cavite and Laguna reported detection of B. bovis , B. bigemina and Theileria spp. in cattle [ 2 , 16 , 25 ]. The limited number of samples as well as the natural resistance of native animals might be attributed to the absence of these TBPs in the current survey.…”

mentioning

confidence: 99%

Molecular survey of tick-borne pathogens infecting backyard cattle and water buffaloes in Quezon province, Philippines

Galon

Ybañez

Moumouni

et al. 2020

The Journal of Veterinary Medical Science

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Tick-borne diseases (TBD) cause enormous losses for farmers. Backyard raising comprises majority of the livestock population in the Philippines, but TBD information in backyard livestock is scarce. In this study, 48 cattle and 114 water buffalo samples from Quezon province, Philippines were molecularly screened for tick-borne pathogens. Anaplasma marginale (16.67%) and hemoplasma (20.99%) were detected in the samples. A. marginale infection ( P =0.0001) was significantly higher in cattle, while hemoplasma infection ( P =0.011) was significantly higher in water buffaloes. A. marginale isolates from this study were highly similar to previous isolates from the Philippines while Mycoplasma wenyonii and Candidatus Mycoplasma haemobos were the identified hemoplasma species. Our findings reveal additional information on the TBD situation of Philippine backyard livestock.

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“…Tick-borne diseases transmitted by Rhipicephalus ticks affect cattle production worldwide, including SEA countries [ 89 , 90 , 91 ]. Studies have shown the potentially devastating impact of R. microplus infestation on developing countries’ livestock economies [ 39 ].…”

Section: The Impacts Of Ticks and Tick-borne Diseasesmentioning

confidence: 99%

Rhipicephalus Tick: A Contextual Review for Southeast Asia

et al. 2021

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Rhipicephalus species are distributed globally with a notifiable presence in Southeast Asia (SEA) within animal and human populations. The Rhipicephalus species are highly adaptive and have established successful coexistence within human dwellings and are known to be active all year round, predominantly in tropical and subtropical climates existing in SEA. In this review, the morphological characteristics, epidemiology, and epizootiology of Rhipicephalus tick species found in SEA are reviewed. There are six commonly reported Rhipicephalus ticks in the SEA region. Their interactions with their host species that range from cattle, sheep, and goats, through cats and dogs, to rodents and man are discussed in this article. Rhipicephalus-borne pathogens, including Anaplasma species, Ehrlichia species, Babesia species, and Theileria species, have been highlighted as are relevant to the region in review. Pathogens transmitted from Rhipicepahalus ticks to host animals are usually presented clinically with signs of anemia, jaundice, and other signs of hemolytic changes. Rhipicephalus ticks infestation also account for ectoparasitic nuisance in man and animals. These issues are discussed with specific interest to the SEA countries highlighting peculiarities of the region in the epidemiology of Rhipicephalus species and attendant pathogens therein. This paper also discusses the current general control strategies for ticks in SEA proffering measures required for increased documentation. The potential risks associated with rampant and improper acaricide use are highlighted. Furthermore, such practices lead to acaricide resistance among Rhipicephalus species are highlighted.

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Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon Island, the Philippines

Cited by 23 publications

References 21 publications

Molecular detection of Anaplasma spp. in blood and milk of dairy cattle in the Philippines

Molecular detection of Anaplasma spp. in blood and milk of dairy cattle in the Philippines

Molecular survey of tick-borne pathogens infecting backyard cattle and water buffaloes in Quezon province, Philippines

Rhipicephalus Tick: A Contextual Review for Southeast Asia

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