Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle

Lee, Eunjung; Kim, Eunju; Ratthanophart, Jadsada; Vitoonpong, Ratchaneekorn; Kim, Bo-Hye; Cho, In‐Soo; Song, Jae‐Young; Lee, Kyoung-Ki; Shin, Yeun-Kyung

doi:10.1016/j.meegid.2016.04.010

Cited by 53 publications

(78 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Infection with BLV may remain clinically silent in an aleukemic form, but about 30% of infected cattle develop persistent lymphocytosis, characterized by polyclonal expansion of B cells, with only a few percent developing lymphoid tumours [1]. BLV infection has a worldwide distribution, and epidemiological studies based on serology show high prevalence in North and South America, some Asiatic and Middle Eastern countries, and Eastern Europe [2–5]. Successful eradication programs have eliminated the disease from most Western European countries, giving these countries a significant trading advantage.…”

Section: Introductionmentioning

confidence: 99%

“…Studies of the genetic diversity of the env gene of BLV isolates from different geographical locations initially revealed eight different genotypes [2, 6–9]. Recently, Lee et al [10] and Polat et al [11], using whole-genome next-generation sequencing (NGS) analysis, performed a comparative analysis of the env sequences and identified two new genotypes, bringing the total to ten (e.g., G1-G10).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of bovine leukemia virus from Moldovan dairy cattle

et al. 2017

View full text Add to dashboard Cite

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cattle from three farms in three regions of Moldova were screened by ELISA and nested PCR. Of these cattle, 14 which were PCR positive, and these were molecularly characterized based on the nucleotide sequence of the env gene and the deduced amino acid sequence of the encoded gp51 protein. Our results demonstrated a low level of genetic variability (0-2.9%) among BLV field strains from Moldova, in contrast to that observed for other retroviruses, including human immunodeficiency virus (HIV) (20-38%) Mason IL (Trudy vologod moloch Inst 146–164, 1970) and equine infectious anemia virus (EIAV) (~40%) Willems L et al (AIDS Res Hum Retroviruses 16(16):1787–1795, 2000), where the envelope gene exhibits high levels of variation Polat M et al (Retrovirology 13(1):4, 2016). Sequence comparisons and phylogenetic analysis revealed that BLV genotype 7 (G7) is predominant in Moldova and that the BLV population in Moldovan cattle is a mixture of at least three new sub-genotypes: G7D, G7E and G4C. Neutrality tests revealed that negative selection was the major force operating upon the 51-kDa BLV envelope surface glycoprotein subunit gp51, although one positively selected site within conformational epitope G was detected in the N-terminal part of gp51. Furthermore, two functional domains, linear epitope B and the zinc-binding domain, were found to have an elevated ratio of nonsynonymous to synonymous codon differences. Together, these data suggest that the evolutionary constraints on epitopes G and B and the zinc-binding domains of gp51 differ from those on the other domains, with a tendency towards formation of homogenous genetic groups, which is a common concept of global BLV diversification during virus transmission that may be associated with genetic drift.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of bovine leukemia virus from Moldovan dairy cattle

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Analysis of BLV gp51 env gene sequences from different locations throughout the world revealed the presence of different genetic groups that correlated with geographic origin [12, 21], and thus, the nucleotide and amino acid sequences composition of gp51 are useful genomic markers of BLV in studies of geographical distribution [9]. A phylogenetic study of BLV env gene has so far identified at least 10 genotypes in the world [5, 7, 8, 14, 16, 19]. …”

mentioning

confidence: 99%

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

Marawan

Mekata

Hayashi

et al. 2017

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

To understand how the latest dominant bovine leukemia virus (BLV) strains were introduced and spread in the Miyazaki prefecture, we collected blood samples from 3 geographic areas (north, central and south) and carried out sequence analysis of the BLV env gene. Two genotypes, genotype I, and III, were identified and the majority of the strains belonged to genotype I (71/74). To clarify a route of BLV introduction, we divided the strains into 20 subgenotypes based on their nucleotide sequences and performed phylogenetic analysis. Our study indicated that common BLV strains were comparatively evenly distributed even in the area, where the farmers have not introduced cattle from other areas and the cattle have limited exposure to BLV infection in grazing fields.

show abstract

“…Genotypes shown on the right are according to Lee et al, Polat et al, and Moratorio et al [21, 22, 27, 28]. Numbers at the branches show bootstrap support (1,000 replicates).…”

Section: Resultsmentioning

confidence: 99%

“…Based on env sequences, various genetic groupings have been described [9–16]. In 2009, Rodriguez et al [17] used new and existing sequence data to differentiate BLV into seven genotypes and subsequently three new genotypes were described in Croatia [18], Bolivia [19], Eastern Europe and Siberia [20], Thailand [21] and Myanmar [22] to bring the total number of described genotypes to 10. Although BLV has been described on all continents, there is little information on BLV infections in the Caribbean region.…”

Section: Introductionmentioning

confidence: 99%

First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

et al. 2016

View full text Add to dashboard Cite

Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis. To investigate the presence and genetic variability of BLV in the Caribbean for the first time, we preformed fluorescence resonance energy transfer (FRET)-PCR for the pol of BLV on DNA from whole blood of cattle from Dominica, Montserrat, Nevis and St. Kitts. Standard PCRs with primers for the env were used for phylogenetic analysis of BLV in positive animals. We found FRET-PCR positive cattle (12.6%, 41/325) on Dominica (5.2%; 4/77) and St. Kitts (19.2%; 37/193) but not on Montserrat (0%, 0/12) or Nevis (0%, 0/43). Positive animals were cows on farms where animals were raised intensively. Phylogenetic analysis using the neighbor-joining (NJ) method on partial and full-length env sequences obtained for strains from Dominica (n = 2) and St. Kitts (n = 5) and those available in GenBank (n = 90) (genotypes 1–10) revealed the Caribbean strains belonged to genotype 1 (98–100% sequence homology). Ours is the first molecular characterization of BLV infections in the Caribbean and the first description of genotype 1 in the region.

show abstract

Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle

Cited by 53 publications

References 35 publications

Molecular characterization of bovine leukemia virus from Moldovan dairy cattle

Molecular characterization of bovine leukemia virus from Moldovan dairy cattle

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

Contact Info

Product

Resources

About