Molecular epidemiological and phylogenetic analyses of canine parvovirus in domestic dogs and cats in Beijing, 2010–2013

Wu, Jing; Gao, Xintao; Hou, Shaohua; Guo, Xiaoyu; Yang, Xue-Shong; Yuan, Weifeng; Xin, Ting; Zhu, Hongfei; Ji, Hong

doi:10.1292/jvms.14-0665

Cited by 24 publications

(24 citation statements)

References 41 publications

(62 reference statements)

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“…Worldwide, there are also some sporadic identifications of the original CPV-2 [31–34], which were regarded as vaccine strains in the previous study [31]. In this study, eighty samples presented some common aa residues with the original CPV-2 (Table 2).…”

Section: Resultsmentioning

confidence: 63%

“…In fact, after a dog is vaccinated, the modified live virus is able to replicate in the animal’s intestinal mucosa and can be shed in the feces [35, 36]. Although the attenuated CPV-2 vaccine strains are stable in limited passages in the dog (i.e., five or six passages) [37, 38], original CPV-2 or CPV-2-like strains were identified from sick and vaccinated dogs [34, 36]. Although there is no way to rule out PCR errors in sequences retrieved from the public database, any possibility of reversion to virulence among the vaccine strains needs to be taken seriously.…”

Section: Resultsmentioning

confidence: 99%

“…There are some sporadic identifications of the original CPV-2 and CPV-2-like strains. The original CPV-2 were regarded as vaccine strains and CPV-2-like strains have been identified from sick and vaccinated dogs [31, 34]. Therefore, CPV-2-like strains may be the result of the reversion of vaccine strains to virulence.…”

Section: Discussionmentioning

confidence: 99%

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The genetic evolution of canine parvovirus – A new perspective

2017

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To trace the evolution process of CPV-2, all of the VP2 gene sequences of CPV-2 and FPV (from 1978 to 2015) from GenBank were analyzed in this study. Then, several new ideas regarding CPV-2 evolution were presented. First, the VP2 amino acid 555 and 375 positions of CPV-2 were first ruled out as a universal mutation site in CPV-2a and amino acid 101 position of FPV feature I or T instead of only I in existing rule. Second, the recently confusing nomenclature of CPV-2 variants was substituted with a optional nomenclature that would serve future CPV-2 research. Third, After check the global distribution of variants, CPV-2a is the predominant variant in Asia and CPV-2c is the predominant variant in Europe and Latin America. Fourth, a series of CPV-2-like strains were identified and deduced to evolve from modified live vaccine strains. Finally, three single VP2 mutation (F267Y, Y324I, and T440A) strains were caught concern. Furthermore, these three new VP2 mutation strains may be responsible for vaccine failure, and the strains with VP2 440A may become the novel CPV sub-variant. In conclusion, a summary of all VP2 sequences provides a new perspective regarding CPV-2 evolution and the correlative biological studies needs to be further performed.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

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The genetic evolution of canine parvovirus – A new perspective

2017

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“…This aa residue is located inside the capsid, forming part of the beta barrel of the viral capsid (Clegg et al., ). Comparison with VP2 sequences available in the GenBank database shows that this change occurred into CPV‐2a/CPV‐2b sequences from Asia (Chinchkar et al., ; Wu et al., ), Brazil (Pereira, Leal, & Durigon, ) and Europe (Cságola et al., ), including Italy (isolate CPV‐2a/151/212—accession n. ). The same mutation was also found in CPV‐2c strains collected in 2011‐2012 in Uruguay (Pérez et al., ) and Ecuador (Aldaz et al., ).…”

Section: Resultsmentioning

confidence: 99%

Introduction of Asian canine parvovirus in Europe through dog importation

Mira

Purpari

Lorusso

et al. 2017

Transbound Emerg Dis

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Summary Canine parvovirus (CPV) is an important infectious agent of domestic and wild carnivores, responsible for severe and often fatal haemorrhagic gastroenteritis and leukopenia. This paper reports the genomic characterization of a CPV strain collected from a dog recently imported to Italy from Thailand. The virus was detected in all tissue samples collected. The whole genome encompassing the two open reading frames encoding for non‐structural (NS1/NS2) and structural (VP1/VP2) proteins was amplified and sequenced. On the basis of genetic analysis of the VP2 gene, the isolate was characterized as CPV‐2c, but it presented genetic signatures typical of Asian strains. Sequence analysis revealed the presence of amino acid changes never observed in European CPV‐2c strains (NS1: Ile60Val, Tyr544Phe, Glu545Val, Leu630Pro; VP2: Ala5Gly, Phe267Tyr, Tyr324Ile, Gln370Arg). By phylogenetic analysis of full‐length VP2 gene, the analysed strain clustered together with Asian viruses. Therefore, a possible introduction of the virus from Asia through the imported dog was suggested, thus confirming the important role of movement of dogs in the global spread of viruses. In addition, full‐length genome analysis could help better trace the spread of canine viruses through different continents.

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“…There has also been a recent CPV-2c epidemic in Vientiane, Laos [64] and Taiwan [65] . In China's capital city, Beijing, CPV-2a and 2b remain the dominant types of this virus [47,[66][67][68] , but more recently, the presence of CPV-2c has been confirmed in China [9,[69][70][71] . In India and Iran, the dominant types in recent outbreaks were CPV-2a and -2b [21,72,73] .…”

Section: Prevalence Of Canine Parvovirus Type 2 Variantsmentioning

confidence: 99%

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

Vannamahaxay¹,

Chuammitri²

2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleVannamahaxay S, Chuammitri P: Update on canine Parvovirus: Molecular and genomic aspects, with emphasis on genetic variants affecting the canine host. Kafkas Univ Vet Fak Derg, 23 (5): 847-856, 2017847-856, . DOI: 10.9775/kvfd.2017 AbstractCanine parvovirus (CPV), the etiology of hemorrhagic enteritis in dogs, was first isolated as CPV type 2 (CPV-2) almost 40 years ago, and was soon replaced by the emergence of new variant types. The major viral capsid proteins encoded by the VP2 gene are the sites where amino acids are often substituted, accounting for the unusual nature of this type of DNA virus. The alteration of specific residues has contributed to different antigenic variants which have affected the evolution of virus binding and host immunity to this virus. Sequence analysis of the VP2 gene and subsequent characterization have revealed three circulating CPV-2 strains, CPV-2a, CPV-2b, and CPV-2c, identified by mutations at amino acid residue 426. The latter strain displays increased pathogenicity in dogs and an extended host range. The present review article aimed at updating contemporary information on epidemiological studies and surveys from CPV field work. Moreover, we pointed out some sensitive and rapid diagnostic tools for detecting CPV in clinical samples, techniques which will be useful for health monitoring and management of CPV with currently available vaccines. Keywords: Canine Parvovirus, CPV type 2, Genetic Variation, VP2 Gene, Mutation, Dog Köpek Parvovirusu Üzerine Bir Güncelleme: Köpek Konakçıya Etki Eden Genetik Varyasyonların Moleküler ve Genomik Özellikleri ÖzetKöpeklerde hemorajik enteritin etiyolojik etkeni olan Canine parvovirus (CPV) neredeyse 40 yıl önce ilk olarak CPV tip 2 (CPV-2) olarak izole edildi ve hemen sonrasında ortaya çıkan yeni varyant tipler CPV tip 2'nin yerine geçti. VP2 geni tarafından kodlanan major viral kapsid proteinler aynı zamanda en sıklıkla amino asitlerin başka amino asitlerle değiştiği alanlar olup bu tip DNA virusların aykırı doğasının da sebebini oluşturmaktadır. Spesifik yapılardaki değişimler farklı antijenik varyantların oluşmasına katkıda bulunarak bu virüsün bağlanma ve virusa karşı konakçı bağışıklığının değişmesini etkilemiştir. VP2 geninin sekans analizi ve takibinde karakterizasyonu, 426. amino asitte mutasyon ile şekillenen CPV2a, CPV-2b ve CPV-2c olmak üzere dolaşımda 3 farklı CPV-2 suşunun olduğunu göstermiştir. CPV-2c köpeklerde artmış patojenite ve daha geniş konakçı yelpazesi göstermektedir. Bu derlemede güncel epidemiyolojik çalışmalar ile CPV saha çalışmaları hakkındaki bilgilerin güncellenmesi amaçlanmıştır. Ayrıca, klinik örneklerde CPV'nin tanısında kullanılmak suretiyle sağlık taramasında faydalı olabilecek ve mevcut aşılarla CPV'nin kontrol altına alınmasında faydalı olabilecek bazı hassas ve hızlı tanı yöntemleri değerlendirilmiştir.

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Molecular epidemiological and phylogenetic analyses of canine parvovirus in domestic dogs and cats in Beijing, 2010–2013

Cited by 24 publications

References 41 publications

The genetic evolution of canine parvovirus – A new perspective

The genetic evolution of canine parvovirus – A new perspective

Introduction of Asian canine parvovirus in Europe through dog importation

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

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