Molecular Epidemiological Analysis of Salmonella enterica Serotype Derby Infections in Hong Kong

Ling, J. M.; Chan, Edward Wai Chi; Cheng, A. F. B.

doi:10.1053/jinf.2001.0806

Cited by 20 publications

(15 citation statements)

References 29 publications

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“…All salmonellae in Hong Kong had remained susceptible to the fluoroquinolones (26)(27)(28)(29). However, we saw a trend of increasing MICs (from 0.03 to 2 g/ml) of fluoroquinolones for salmonellae isolated from 1993 to 1998 (unpublished observation).…”

mentioning

confidence: 87%

Mutations in Topoisomerase Genes of Fluoroquinolone-Resistant Salmonellae in Hong Kong

Ling

Chan

Lam

et al. 2003

Antimicrob Agents Chemother

138

106

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A total of 88 salmonella isolates (72 clinical isolates for which the ciprofloxacin MIC was >0.06 g/ml, 15 isolates for which the ciprofloxacin MIC was <0.06 g/ml, and Salmonella enterica serotype Typhimurium ATCC 13311) were studied for the presence of genetic alterations in four quinolone resistance genes, gyrA, gyrB, parC, and parE, by multiplex PCR amplimer conformation analysis. The genetic alterations were confirmed by direct nucleotide sequencing. A considerable number of strains had a mutation in parC, the first to be reported in salmonellae. Seven of the isolates sensitive to 0.06 g of ciprofloxacin per ml had a novel mutation at codon 57 of parC (Tyr573Ser) which was also found in 29 isolates for which ciprofloxacin MICs were >0.06 g/ml. Thirty-two isolates had a single gyrA mutation (Ser833Phe, Ser833Tyr, Asp873Asn, Asp873Tyr, or Asp873 Gly), 34 had both a gyrA mutation and a parC mutation (29 isolates with a parC mutation of Tyr573Ser and 5 isolates with a parC mutation of Ser803Arg). Six isolates which were isolated recently (from 1998 to 2001) were resistant to 4 g of ciprofloxacin per ml. Two of these isolates had double gyrA mutations (Ser833Phe and Asp873Asn) and a parC mutation (Ser803Arg) (MICs, 8 to 32 g/ml), and four of these isolates had double gyrA mutations (Ser833Phe and Asp873Gly), one parC mutation (Ser803Arg), and one parE mutation (Ser4583Pro) (MICs, 16 to 64 g/ml). All six of these isolates and those with a Ser803Arg parC mutation were S. enterica serotype Typhimurium. One S. enterica serotype Typhi isolate harbored a single gyrA mutation (Ser833Phe), and an S. enterica serotype Paratyphi A isolate harbored a gyrA mutation (Ser833Tyr) and a parC mutation (Tyr573Ser); both of these isolates had decreased susceptibilities to the fluoroquinolones. The MICs of ciprofloxacin, levofloxacin, and sparfloxacin were in general the lowest of those of the six fluoroquinolones tested. Isolates with a single gyrA mutation were less resistant to fluoroquinolones than those with an additional parC mutation (Tyr573Ser or Ser803Arg), while those with double gyrA mutations were more resistant.Salmonellosis remains a major public health problem worldwide. In contrast to gastroenteritis caused by salmonellae, invasive salmonellosis requires prompt antibiotic therapy. The antibiotics used for the treatment of salmonellosis have traditionally been chloramphenicol, sulfamethoxazole-trimethoprim, and ampicillin. However, with the development of resistance to these drugs, fluoroquinolones have been used and have been successful in treating many clinical cases. Unfortunately, fluoroquinolone-resistant strains have rapidly developed in recent years, and treatment failures have been reported (2,8,13,20,37,44,47).Resistance to the fluoroquinolones in salmonellae has mainly been attributed to mutations in the gyrA gene. Mutations have rarely been reported in the gyrB gene, and none have been reported in the parC gene (7,12,36,48,51). Other resistance mechanisms such as efflux and reduced uptake of drugs have also...

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mentioning

confidence: 87%

Mutations in Topoisomerase Genes of Fluoroquinolone-Resistant Salmonellae in Hong Kong

Ling

Chan

Lam

et al. 2003

Antimicrob Agents Chemother

138

106

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“…This serovar is not exclusively adapted to pigs but most often associated with this source. Nevertheless, S. Derby is also isolated from cases of human salmonellosis and from food, mainly pork, and is therefore supposed to spread from pig via pork to human ( [Ling et al, 2001], [Valdezate et al, 2005] and [Zhao et al, 2007]). …”

Section: Introductionmentioning

confidence: 99%

“…The few data available for S. Derby (http://mlst.ucc.ie/mlst/mlst/dbs/Senterica/) indicate that the serovar originates from more than one common ancestor which is called polyphyletic. Moreover, some studies used pulsed-field gel electrophoresis to elucidate the spread of S. Derby in slaughterhouses (Giovannacci et al, 2001), or to investigate the genetic diversity of the serovar in France ( [Kerouanton et al, 2007] and [Kerouanton et al, 2010]), Spain (Valdezate et al, 2005), Hong Kong (Ling et al, 2001), China (Xia et al, 2009) or Brazil (Michael et al, 2006). Clinical, food and animal isolates showed a wide range of PFGE and antimicrobial resistance profiles, often either implicated streptomycin, tetracycline, or sulfonamides ( [Valdezate et al, 2005], [Michael et al, 2006], [Gebreyes et al, 2004] and [Clothier et al, 2010]).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany

Hauser

Hebner

Tietze

et al. 2011

International Journal of Food Microbiology

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“…Uma grande diversidade genética de amostras S. enterica Typhi, isoladas de casos esporádicos e surtos de febre tifóide ou de ambientes, tem sido descrita quando tais amostras são submetidas à tipagem por PFGE (1,3,10,17,18) . A eletroforese de campo pulsado pode ser desenvolvida após (9,5,11,21) .…”

Section: Eletroforese Em Gel De Campo Pulsadounclassified

“…Vários estudos utilizaram a técnica de PFGE para avaliar epidemiologicamente amostras de S. Typhi, tanto de surtos como de casos esporádicos, confirmando a co-existência de múltiplos clones endêmicos (3,10,18) .…”

Section: Eletroforese Em Gel De Campo Pulsadounclassified

Variabilidade genética de amostras de Salmonella Typhi isoladas de surto e de casos esporádicos ocorridos em Belém, Brasil

Bastos¹,

Lima²,

Sá³

et al. 2008

J. Bras. Patol. Med. Lab.

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Molecular Epidemiological Analysis of Salmonella enterica Serotype Derby Infections in Hong Kong

Cited by 20 publications

References 29 publications

Mutations in Topoisomerase Genes of Fluoroquinolone-Resistant Salmonellae in Hong Kong

Mutations in Topoisomerase Genes of Fluoroquinolone-Resistant Salmonellae in Hong Kong

Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany

Variabilidade genética de amostras de Salmonella Typhi isoladas de surto e de casos esporádicos ocorridos em Belém, Brasil

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