Molecular Effects of Uptake of Gold Nanoparticles in HeLa Cells

Potential of rod, sphere and semi-cube shaped gold nanoparticles to induce cytotoxicity and genotoxicity in human blood lymphocytes in vitro

“…Only low concentration of BR-GNPs did not induce cytotoxic effects in lymphocytes. These results were in agreement with the previous works which demonstrated that nanorods did not induce significant toxicity in Hela cells (49,50). However, high concentrations of nanorods induced cytotoxicity in fibroblasts, epithelial cells and melanoma in concentrator dependent manner (51).…”

Gold nanoparticle-mediated gene delivery induces widespread changes in the expression of innate immunity genes

“…Electron microscopic morphological studies of trafficking of vesicles in the endolysosomal system suggest that this cell structure is formed by direct fusion of late endosomes and lysosomes to create a hybrid organelle for the digestion of endocytosed macromolecules. [11][12][13][14] Figure 1 Gold nanoparticle-oligonucleotide complexes are localized within membrane-bound organelles with electron-dense cores. (a) PBMCs were treated with a 10 nM concentration of gold nanoparticle-oligonucleotide complexes with a 5¢ Cy5.5-label for 24 h. Representative cells from independent healthy blood donors are shown (Cy5.5 fluorescence is red, actin is green and DNA is blue).…”

“…To match other studies of the HeLa cell response to these gold nanoparticle-oligonucleotide complexes, we performed genome-wide expression profiling of PBMCs and 293T cells treated with gold nanoparticle-oligonucleotide complexes. 12,16 Three of the five PBMC conditions (24 and 48 h after treatment with the gold nanoparticle-oligonucleotide complexes and the negative control, respectively) were assayed independently a second and a third time (technical and biological replicates, respectively) to control for experimental variability (15 microarrays hybridized in total). A different batch of these same gold nanoparticle-oligonucleotide complexes was used in the generation of the four 293T cell samples and the three biologically replicate PBMC samples, and the microarrays for these samples with calibration controls included were processed separately.…”

Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice

“…A variety of structures with unique structural [9,10], optical [11,12], electronic [13], magnetic [14], and catalytic [15] properties have been exploited in the areas of cancer imaging [2,[16][17][18][19], diagnostics [6,20,21], and treatment [22][23][24][25][26][27][28][29][30]. Noble metal nanoparticles provide remarkable opportunities in these applications due to their inherently low toxicity [31][32][33] and strongly enhanced optical properties associated with localized surface plasmon resonance [34][35][36]. The enhanced electromagnetic field surrounding such particles gives rise to large absorption, Rayleigh (Mie) scattering, raman scattering, and twophoton luminescence cross-sections, properties which have been utilized in photothermal cancer therapy [24][25][26][27][28][29][30] (PTT), surface enhanced Raman detection [37][38][39] (SERS), and diagnostic imaging [17][18][19][20] applications...…”