Molecular effectors of multiple cell death pathways initiated by photodynamic therapy

Buytaert, Esther; Dewaele, Michael; Agostinis, Patrizia

doi:10.1016/j.bbcan.2007.07.001

Cited by 519 publications

(694 citation statements)

References 198 publications

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“…Light (centred at 630 nm, 75 J/cm 2 in our practice, although 37-150 J/cm 2 may be used) is applied to excite PpIX from the ground state. The absorption of light leads to a singlet state that can undergo intersystem crossing to a long-lived triplet state which can in turn excite molecular oxygen leading to the generation of singlet oxygen and other reactive oxygen species (ROS) that induce oxidative stress and cell death 8 . Alternatively the singlet state of PpIX can decay back to the ground state resulting in fluorescence 8 .…”

Section: Introductionmentioning

confidence: 99%

“…The absorption of light leads to a singlet state that can undergo intersystem crossing to a long-lived triplet state which can in turn excite molecular oxygen leading to the generation of singlet oxygen and other reactive oxygen species (ROS) that induce oxidative stress and cell death 8 . Alternatively the singlet state of PpIX can decay back to the ground state resulting in fluorescence 8 . Fluorescence is therefore an indicator of the concentration of PpIX in tissue and can be used in PDT diagnostics and clinical research 8,9 .…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively the singlet state of PpIX can decay back to the ground state resulting in fluorescence 8 . Fluorescence is therefore an indicator of the concentration of PpIX in tissue and can be used in PDT diagnostics and clinical research 8,9 . The efficacy of PDT depends on the combination of the three key treatment parameters: PpIX distribution, molecular oxygen and light delivery 10 .…”

Section: Introductionmentioning

confidence: 99%

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Development of a handheld fluorescence imaging device to investigate the characteristics of protoporphyrin IX fluorescence in healthy and diseased skin

Kulyk

Ibbotson

Moseley

et al. 2015

Photodiagnosis and Photodynamic Therapy

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3 HighlightsWe developed a handheld device and a time course method for fluorescence imagingThe device can be used to study PpIX formation in healthy and diseased skin PpIX fluorescence grows throughout the 3 hours prior to treatmentThe fluorescence intensity varies between diagnoses and individuals The fluorescence depends on the body site, possibly due to differences in temperature 4 Abstract Background

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a handheld fluorescence imaging device to investigate the characteristics of protoporphyrin IX fluorescence in healthy and diseased skin

Kulyk

Ibbotson

Moseley

et al. 2015

Photodiagnosis and Photodynamic Therapy

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“…O estado tripleto tem meia vida mais longa (de micro a milissegundos) e pode reagir com o oxigênio celular por intermédio de dois mecanismos classificados como "tipo I" e "tipo II". Ambos os mecanismos podem ocorrer simultaneamente, e a taxa entre os dois irá depender do tipo de Fs usado e das concentrações de substrato e oxigênio (Buytaert et al, 2007;Castano et al, 2004). Na reação do tipo I, o Fs, em seu estado tripleto, reage com substratos biológicos formando radicais livres, os quais reagem, por sua vez, com o oxigênio molecular originando radicais de anion superóxido (O2 -), peróxido de hidrogênio (H2O2) e radicais hidroxilas (OH -).…”

Section: óXido Nítrico Sintase Induzível (Inos)unclassified

“…A Bcl-2, em níveis normais, reage com a beclin e previne que essa proteína inicie a formação do autofagossomo. Assim, ao ser inibida a Bcl-2 pela PDT, a formação do autofagossomo pode ser desencadeada (Buytaert et al, 2007;Reiners et al, 2010).…”

Section: óXido Nítrico Sintase Induzível (Inos)unclassified

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

Rosin¹

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A minha Orientadora, Prof a . Dr a . Luciana Corrêa, por sempre ouvir e discutir comigo todas as questões e dúvidas que surgiram durante o desenvolvimento desta tese e por ser essa pessoa tão generosa, inteligente e acessível. Admiro-a muito como pessoa e pesquisadora.Ao Prof. Dr. Moacir Novelli, que durante suas visitas ao laboratório sempre alegrava meu dia e por compartilhar comigo um pouco da sua imensa sabedoria. A Marina, a única companheira de profissão da Pós, que sempre esteve disposta a me ajudar e me ouvir, pela amizade, carinho e companheirismo. Pelos nossos cafés da manhã que sempre tornava meu dia mais leve e agradável.A Karla, pelo bom humor constante que sempre alegrava meu dia, por sempre me ouvir, me apoiar e pelos nossos cafezinhos.A Gabriela, por sempre estar ao meu lado me dando força e apoio, pelos nossos almoços diários na copa do departamento que tornavam meu dia mais alegre e por todo carinho e amizade.A Lara, pela amizade e por todo carinho e apoio. Por ser essa pessoa tão agradável e acessível por sempre estar disposta a me ourvir. Tenho a impressão de ter sido uma criança brincando à beira-mar, divertindo-me em descobrir uma pedrinha mais lisa ou uma concha mais bonita que as outras, enquanto o imenso oceano da verdade continua misterioso diante de meus olhos". Oral squamous cell carcinoma (SCC) is a malignant tumor with high morbidity and mortality rates, and it is difficult to treat. Conventional treatment for oral SCCs includes surgery and radiotherapy that may be followed by chemotherapy. Although these treatments are widely used, they are ineffective against some resistant tumors. Isaac Newton RESUMOOncologic photodynamic therapy (PDT) has been used as an adjuvant treatment for oral SCCs, especially in less invasive cases that require tumor reduction before surgical resection. However, like conventional treatments, PDT can induce the occurrence of resistant cell populations such as cutaneous carcinomas and colon and breast adenocarcinomas. The hypothesis that oral SCCs develop resistance to PDThas not yet been tested. Therefore, the aims of this study were to investigate whether oral SCCs (SCC9) develop resistance to several cycles of 5-aminolevulinic acidmediated PDT (5-ALA-PDT) and to determine whether the expression of markers associated with cell survival (NFB, Bcl-2, iNOS, mTOR, and Akt) is altered during this process. An oral SCC (SCC9) cell line was used, which was subjected to the following conditions: 1) Control: cultured without any treatment; 2) ALA: incubated with 5-ALA (1 mM for 4 h); 3) LED: treated with LED light (630 nm, 5.86 J/cm 2 , 22.5 J, 150 mW, 150 s); and 4) PDT: treated with 5-ALA-PDT (with the protocols of the ALA and LED groups combined) generating a lethal dose of 90%. Initially, only one cycle of PDT was administered, and cell viability was determined in all groups 24, 48, 72, and 120 h after irradiation. Subsequently, the DNA fragmentation detection assay (TUNEL) and immunofluorescence analysis of the expression of proteins NFB, Bcl-2, iNOS, pmTOR, ...

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The Application of KillerRed for Acute Protein Inactivation in Living Cells

Jarvela

Linstedt

2014

CP Cytometry

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Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live‐cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live‐cell imaging of cultured cells. Curr. Protoc. Cytom. 69:12.35.1‐12.35.10. © 2014 by John Wiley & Sons, Inc.

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Molecular effectors of multiple cell death pathways initiated by photodynamic therapy

Cited by 519 publications

References 198 publications

Development of a handheld fluorescence imaging device to investigate the characteristics of protoporphyrin IX fluorescence in healthy and diseased skin

Development of a handheld fluorescence imaging device to investigate the characteristics of protoporphyrin IX fluorescence in healthy and diseased skin

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

The Application of KillerRed for Acute Protein Inactivation in Living Cells

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