Molecular Ecology of Isoprene-Degrading Bacteria

Carrión, Ornella; McGenity, Terry J.; Murrell, J. Colin

doi:10.3390/microorganisms8070967

Cited by 12 publications

(19 citation statements)

References 92 publications

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“…Prior to this study, little was known about isoprene-degrading microorganisms in tropical soils, with just a few studies analysing isoprene degraders in soil associated with oil palm trees, but growing in temperate environments [ 52 , 53 ], or in soil transported to the UK [ 20 ]. Oil-palm soil was found to have the highest abundance of isoprene monooxygenase genes ( isoA ), determined by quantitative PCR [ 20 , 54 ]. In these studies, genera such as Rhodococcus, Gordonia, Novosphingobium, Pelomonas, Rhodoblastus, Sphingomonas and Zoogloea were identified as isoprene-degrading bacteria by DNA stable isotope probing [ 20 , 52 , 53 , 55 ].…”

Section: Resultsmentioning

confidence: 99%

“…Many of these genera have typically been found to increase in isoprene-enriched soils or have been recognised as isoprene degraders, such as Rhodococcus , Comamonas and Pseudomonas [ 17 , 20 , 21 , 54 , 56 ]. In contrast, Acinetobacter has not been shown to have an isoprene-degrading capacity, although it is a widespread genus with broad catabolic capabilities, including the capacity to grow on short-chain alkanes [ 57 ] and alkenes [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

“…In these studies, genera such as Rhodococcus, Gordonia, Novosphingobium, Pelomonas, Rhodoblastus, Sphingomonas and Zoogloea were identified as isoprene-degrading bacteria by DNA stable isotope probing [20,52,53,55]. A wider variety of genera has also been implicated in isoprene degradation in temperate soils [17,19,20,54,55]. Enrichment with isoprene led to a convergence in, and a significantly different, bacterial community composition compared with the soils that were not incubated with isoprene (pre-enrichment treatments) (F 1,50 = 3.746, p < 0.05; Figure 2d).…”

Section: Bacterial Community Analysis and Identification Of Taxa That Increase In Relative Abundance After Isoprene Enrichmentmentioning

confidence: 99%

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Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees

et al. 2021

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Isoprene, a volatile hydrocarbon emitted largely by plants, plays an important role in regulating the climate in diverse ways, such as reacting with free radicals in the atmosphere to produce greenhouse gases and pollutants. Isoprene is both deposited and formed in soil, where it can be consumed by some soil microbes, although much remains to be understood about isoprene consumption in tropical soils. In this study, isoprene-degrading bacteria from soils associated with tropical plants were investigated by cultivation and cultivation-independent approaches. Soil samples were taken from beneath selected framework forest trees and economic crops at different seasons, and isoprene degradation in soil microcosms was measured after 96 h of incubation. Isoprene losses were 4–31% and 15–52% in soils subjected to a lower (7.2 × 105 ppbv) and a higher (7.2 × 106 ppbv) concentration of isoprene, respectively. Sequencing of 16S rRNA genes revealed that bacterial communities in soil varied significantly across plant categories (framework trees versus economic crops) and the presence of isoprene, but not with isoprene concentration or season. Eight isoprene-degrading bacterial strains were isolated from the soils and, among these, four belong to the genera Ochrobactrum, Friedmanniella, Isoptericola and Cellulosimicrobium, which have not been previously shown to degrade isoprene.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Bacterial Community Analysis and Identification Of Taxa That Increase In Relative Abundance After Isoprene Enrichmentmentioning

confidence: 99%

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Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees

et al. 2021

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“…The aims of this review are to summarize what is known about aquatic isoprene cycling and to highlight the poten-tial for further study of the ecology of isoprene-degrading microbes and the biological consumption of isoprene in aquatic environments. Our understanding of the physiology, biochemistry, mole cular biology and ecology of isoprene-degrading bacteria has focussed mostly on terrestrial environments (re viewed by Mc-Genity et al 2018, Carrión et al 2020b, and so we summarise what is known about the biology of isoprene consumption using examples mainly from the terrestrial environment and then focus on aquatic environments.…”

Section: Introductionmentioning

confidence: 99%

The microbiology of isoprene cycling in aquatic ecosystems

Dawson

Crombie

Pichon

et al. 2021

Aquat. Microb. Ecol.

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Isoprene (2-methyl-1,3-butadiene) is emitted in vast quantities (>500 Tg C yr-1). Most isoprene is emitted by trees, but there is still incomplete understanding of the diversity of isoprene sources. The reactivity of isoprene in the atmosphere has potential implications for both global warming and global cooling, with human health implications also arising from isoprene-induced ozone formation in urban areas. Isoprene emissions from terrestrial environments have been studied for many years, but our understanding of aquatic isoprene emissions is less complete. Several abundant phytoplankton taxa produced isoprene in the laboratory, and the relationship between chlorophyll a and isoprene production has been used to estimate emissions from marine environments. The aims of this review are to highlight the role of aquatic environments in the biological cycling of isoprene and to stimulate further study of isoprene metabolism in marine and freshwater environments. From a microbial ecology perspective, the isoprene metabolic gene cluster, first identified in Rhodococcus sp. AD45 (isoGHIJABCDEF) and subsequently found in every genome-sequenced isoprene-degrader, provides the ideal basis for molecular studies on the distribution and diversity of isoprene-degrading communities. Further investigations of isoprene-emitting microbes, such as the influence of environmental factors and geographical location, must also be considered when attempting to constrain estimates of the flux of isoprene in aquatic ecosystems. We also report isoprene emission by the scleractinian coral Acropora horrida and the degradation of isoprene by the same coral holobiont, which highlights the importance of better understanding the cycling of isoprene in marine environments.

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“…It has been known for a number of years that soils can be a sink for isoprene [ 14 , 15 ] and this has stimulated research on the microbiology of isoprene metabolism. The biological consumption of isoprene by bacteria and their role in the isoprene cycle has been reviewed in detail recently ([ 16 , 17 , 18 ]). In brief, a number of Gram-positive bacteria, mainly Actinobacteria of the genera Rhodococcus [ 19 , 20 ], Gordonia , Mycobacterium [ 21 ], and Nocardioides [ 22 ] have been isolated and characterized.…”

Section: Introductionmentioning

confidence: 99%

Sphingopyxis sp. Strain OPL5, an Isoprene-Degrading Bacterium from the Sphingomonadaceae Family Isolated from Oil Palm Leaves

et al. 2020

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The volatile secondary metabolite, isoprene, is released by trees to the atmosphere in enormous quantities, where it has important effects on air quality and climate. Oil palm trees, one of the highest isoprene emitters, are increasingly dominating agroforestry over large areas of Asia, with associated uncertainties over their effects on climate. Microbes capable of using isoprene as a source of carbon for growth have been identified in soils and in the tree phyllosphere, and most are members of the Actinobacteria. Here, we used DNA stable isotope probing to identify the isoprene-degrading bacteria associated with oil palm leaves and inhabiting the surrounding soil. Among the most abundant isoprene degraders of the leaf-associated community were members of the Sphingomonadales, although no representatives of this order were previously known to degrade isoprene. Informed by these data, we obtained representatives of the most abundant isoprene degraders in enrichments, including Sphingopyxis strain OPL5 (Sphingomonadales), able to grow on isoprene as the sole source of carbon and energy. Sequencing of the genome of strain OPL5, as well as a novel Gordonia strain, confirmed their pathways of isoprene degradation and broadened our knowledge of the genetic and taxonomic diversity of this important bacterial trait.

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Molecular Ecology of Isoprene-Degrading Bacteria

Cited by 12 publications

References 92 publications

Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees

Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees

The microbiology of isoprene cycling in aquatic ecosystems

Sphingopyxis sp. Strain OPL5, an Isoprene-Degrading Bacterium from the Sphingomonadaceae Family Isolated from Oil Palm Leaves

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