Molecular ecology of <i>Schistosoma mansoni</i> transmission inferred from the genetic composition of larval and adult infrapopulations within intermediate and definitive hosts

Thèron, André; Sire, C.; Rognon, Anne; Prugnolle, Franck; Durand, Patrick

doi:10.1017/s0031182004005943

Cited by 75 publications

(81 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Presence of multiple genotypes of S. mansoni within a single snail (Biomphalaria glabrata) has been reported by Minchella et al (1995), Sire et al (1999), Eppert et al (2002) and Theron et al (2004). For the Schistosoma haematobium / Bulinus spp.…”

Section: Discussionmentioning

confidence: 85%

Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses

Yin

et al. 2008

International Journal for Parasitology

View full text Add to dashboard Cite

We genotyped (using 16 or 17 microsatellite loci) numerous adult Schistosoma japonicum raised in rabbits exposed to pooled cercariae from small numbers of naturally infected snails from several localities in China. As expected, duplicate multi-locus genotypes (MLGs) were found among these worms. Additionally, many more MLGs, often near-identical, were found than snails used as sources of cercariae. Explanations for these results include i) genotyping errors, ii) development within each infected snail of multiple sibling miracidia and iii) somatic mutation producing genetically varied cercariae from a single miracidium. To control for genotyping errors we re-analysed samples from many individual worms, including repeating the initial PCR. Explanations invoking the development of multiple sibling miracidia within a single snail are not likely to be correct because almost all duplicate MLGs fell within same-sex clusters in a principal coordinates analysis. We would expect both sexes to be represented in a multi-miracidium infection. In addition, we exposed several snails to infection by a single miracidium. One such snail, via an experimentally infected mouse, yielded 48 adult worms. The presence of at least nine near-identical MLGs among these worms was confirmed by re-genotyping. We regard somatic mutation as the most likely explanation for our results. The implications of multiple MLGs for population-genetic studies in S. japonicum are discussed.

show abstract

Section: Discussionmentioning

confidence: 85%

Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses

Yin

et al. 2008

International Journal for Parasitology

View full text Add to dashboard Cite

show abstract

“…hepatica], there is a high risk that eggs will be deposited in habitats that are unsuitable for transmission. This increases the variance in reproductive success between genotypes, which could explain the relatively low levels of genetic variability and relatively high population genetic structure (Mulvey et al 1991, Théron et al 2004, Prugnolle et al 2005c, Vilas et al 2012). However, purely aquatic species always deposit their eggs in water and the aquatic environment is conducive to larval dispersal, thereby enhancing the possibility of gene flow (Criscione and Blouin 2006).…”

Section: Transmission Environmentmentioning

confidence: 99%

Influence of life history traits on the population genetic structure of parasitic helminths: a minireview

Vázquez-Prieto

Vilas

Paniagua

et al. 2015

Folia Parasitologica

View full text Add to dashboard Cite

Parasite life history traits influence the rate of gene flow between populations and the effective population size, both of which determine the levels of genetic variability and the geographic distribution of such variability. In this short review targeted to parasitologists, we summarise how life history traits influence the population genetic structure of parasitic helminths. These organisms are characterised by a wide variety of life cycles and are ecologically different from microparasites, which have been studied in more detail. In order to provide the reader a concise review that illustrates key aspects of the subject matter, we have limited ourselves to studying examples selected for their clarity and relevance.

show abstract

“…Asexual reproduction in intermediate hosts does not necessarily lead, however, to a stable recurrence of generations within, and enhanced genetic diversity among definitive hosts, because it will be countered by factors such as reduced variance in reproductive success between clones and enhanced mobility of both definitive and intermediate hosts [74]. Theron et al [75] for example, found that infrapopulations of Schistosoma mansoni in rats (Rattus rattus) from Guadaloupe, contained a mean of 34 different multilocus genotypes per host despite the fact that snail intermediate hosts (Biomphalaria glabrata) contained only 1.1 genotypes per host, on average. The transmission of multiple genotypes to the definitive host is likely due to the mobility of rats, their weak immune response, allowing multiple infections, and to spatial aggregation of infected snails around limited water resources.…”

Section: Fragmentation Among Hostsmentioning

confidence: 99%

The molecular epidemiology of parasite infections: Tools and applications

Lymbery

Thompson

2012

Molecular and Biochemical Parasitology

View full text Add to dashboard Cite

Molecular epidemiology, broadly defined, is the application of molecular genetic techniques to the dynamics of disease in a population. In this review, we briefly describe molecular and analytical tools available for molecular epidemiological studies and then provide an overview of how they can be applied to better understand parasitic disease. A range of new molecular tools have been developed in recent years, allowing for the direct examination of parasites from clinical or environmental samples, and providing access to relatively cheap, rapid, high throughput molecular assays. At the same time, new analytical approaches, in particular those derived from coalescent theory, have been developed to provide more robust estimates of evolutionary processes and demographic parameters from multilocus, genotypic data. To date, the primary application of molecular epidemiology has been to provide specific and sensitive identification of parasites and to resolve taxonomic issues, particularly at the species level and below. Population genetic studies have also been used to determine the extent of genetic diversity among populations of parasites and the degree to which this diversity is associated with different host cycles or epidemiologically important phenotypes. Many of these studies have also shed new light on transmission cycles of parasites, particularly the extent to which zoonotic transmission occurs, and on the prevalence and importance of mixed infections with different parasite species or intraspecific variants (polyparasitism). A major challenge, and one which is now being addressed by an increasing number of studies, is to find and utilise genetic markers for complex traits of epidemiological significance, such as drug resistance, zoonotic potential and virulence.

show abstract

Molecular ecology of Schistosoma mansoni transmission inferred from the genetic composition of larval and adult infrapopulations within intermediate and definitive hosts

Cited by 75 publications

References 23 publications

Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses

Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses

Influence of life history traits on the population genetic structure of parasitic helminths: a minireview

The molecular epidemiology of parasite infections: Tools and applications

Contact Info

Product

Resources

About