Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping

Lima-Oliveira, Tarcianne M; Fontes, Fernanda von Hertwig Mascarenhas; Lilioso, Maurício; Pires-Silva, Dayane; Teixeira, Marta Maria Geraldes; Vergara-Meza, Jose Gabriel; Harry, Myriam; Filée, Jonathan; Costa, Jane; Valença-Barbosa, Carolina; Folly-Ramos, Elaine; Almeida, Carlos Eduardo

doi:10.1016/j.actatropica.2019.105188

Cited by 19 publications

(23 citation statements)

References 38 publications

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“…It was also shown that this high prevalence of natural infection is combined with high populational densities of T. brasiliensis in this state if compared to Paraíba [8]. These findings were confirmed by several authors throughout multiples approaches [23,43,44,45,46,47]. Noteworthy, even the populations that fed on cavies were not highly infected in Paraíba state.…”

Section: Discussionsupporting

confidence: 68%

“…Five sylvatic populations were found to feed on reptiles and one peridomestic. Recently, it was found that reptiles are the main food source (86%) for T. petrocchiae [45]-a triatomine that cohabits with T. brasiliensis [23]. Indeed, sporadic blood meals on coldblooded animals (such as frogs) have already been observed for T. brasiliensis [5].…”

Section: Discussionmentioning

confidence: 99%

“…In all set of PCRs we included a DNA extracted from a T. cruzi (TcI) culture as a positive control. Additionally, the negative controls used were NTC (non-template control) and a T. rangeli DNA identified and isolated from T. brasiliensis [23]. We assumed that these DNAs did not have inhibitors for PCR amplification as we used the same insects' DNAs that were successfully amplified for feeding source detections (see the previous topic).…”

Section: Natural Infection By Trypanosoma Cruzimentioning

confidence: 99%

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Dynamics of food sources, ecotypic distribution and Trypanosoma cruzi infection in Triatoma brasiliensis from the northeast of Brazil

et al. 2020

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Innovative approaches used to combat Chagas disease transmission tend to combine a set of comprehensive efforts to understand the ecology of local vectors. In this work we identified molecularly the blood meal of 181 Triatoma brasiliensis, distributed in 18 populations (8 sylvatic and 10 peridomestic), which were collected across a range of 240 km (East-West) and 95 km (North-South) in the semi-arid region of northeastern, Brazil. We used the vertebrate mitochondrial gene (cytochrome B) sequencing applied to DNA isolated from bug midgut to identify the insect blood meal sources via the BLAST procedure. The peridomestic populations were classified according to two main hypotheses of site-occupancy for T. brasiliensis: the first says that the infestation is mainly driven by structures that resemble its natural habitat (stony-like ecotopes) and the second assumes that it is associated with key-hosts (rodents and goats). Rodents of the Caviidae family (Galea spixii and Kerodon rupestris) were identified as the key-host of T. brasiliensis, but also the potential Trypanosoma cruzi reservoir-able to connect the sylvatic and domestic T. cruzi cycle. Cats also deserve to be studied better, as potential T. cruzi reservoirs. By modeling the food sources + site-occupancy + T. cruzi natural infection, we identified man-made ecotopes suitable for forming dense triatomine infestations with high rates of T. cruzi natural infection, which may be taken into account for vector control measures.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Natural Infection By Trypanosoma Cruzimentioning

confidence: 99%

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Dynamics of food sources, ecotypic distribution and Trypanosoma cruzi infection in Triatoma brasiliensis from the northeast of Brazil

et al. 2020

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“…These last authors also reported di culty of differentiating T. cruzi from other morphologically similar protozoa (Blastocrithidia triatomae e T. rangeli). It is important to note that T. rangeli has already been found in T. brasiliensis in the Rio Grande do Norte (Lima-Oliveira et al, 2019). However, given the lack of statistical evidence, we suggest the OM is still valid to estimate T. cruzi prevalence if insects are fresh and alive.…”

Section: Discussionmentioning

confidence: 79%

Eco-epidemiologic profile of an area of a Chagas disease outbreak: the risk of oral transmissions in the Brazilian northeast

Lara

Lilioso

Valença-Barbosa

et al. 2020

Preprint

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Chagas disease is a neglected tropical disease strongly associated with low socioeconomic status, affecting nearly 8 million people – mainly Latin Americans. The current infection risk is based on acute case reports, most of which are typically associated with oral transmissions. In the semi-arid region of Northeastern Brazil, serious outbreaks of this transmission type have surged in the last years. One of those occurred in the city of Marcelino Vieira (2016), in the state of Rio Grande do Norte. Rural residents of four municipalities surrounding Marcelino Vieira ingested sugar cane juice – which was probably ground with Trypanosoma cruzi infected insects. The structure of domiciliary unities (DUs) in the rural area of Marcelino Vieira was investigated to better understand the factors related to the outbreaks in this region – which was combined with entomological indicators. We found triatomines (mainly Triatoma brasiliensis) in 54% (36/67) of DUs and all rocky outcrops inspected (n = 7). Overall, 26% (119/458) of examined T. brasiliensis were infected by T. cruzi in artificial ecotopes, with almost the same prevalence in the sylvatic environment (23%; 35/154). The local variation in T. cruzi prevalence (variating from 0%-100%) was highly correlated with the presence of some ecotopes where the insects were found; and we identified those linked to high natural triatomine infection prevalence by T. cruzi (mainly wood/tile/brick piles). Ninety-five percent of people interviewed recognized the triatomines and knew the classic route (vector-borne) of transmission of disease. However, only 7.5% admitted knowledge that Chagas disease can also be acquired orally – which poses a risk this transmission route currently recognized. Here, we highlight the physical proximity between humans and infected vector populations as an additional risk factor to oral/vector contaminations, providing recommendations to avoid further outbreaks.

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“…Triatoma lenti was notified in two Brazilian states, namely, Bahia and Goia ´s [31,51,53]. Triatoma sherlocki is an endemic species from Bahia [50,54]. Finally, the subspecies T. b. macromelasoma, described based on melanic forms of T. b. brasiliensis found at home in the state of Pernambuco [31,51], was redescribed and revalidated by Costa et al [42].…”

Section: Introductionmentioning

confidence: 99%

Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

et al. 2021

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Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.

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Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping

Cited by 19 publications

References 38 publications

Dynamics of food sources, ecotypic distribution and Trypanosoma cruzi infection in Triatoma brasiliensis from the northeast of Brazil

Dynamics of food sources, ecotypic distribution and Trypanosoma cruzi infection in Triatoma brasiliensis from the northeast of Brazil

Eco-epidemiologic profile of an area of a Chagas disease outbreak: the risk of oral transmissions in the Brazilian northeast

Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

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