Molecular diversity study of black cumin (Nigella sativa L.) from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers

Abstract

Nigella sativa L. (commonly known as black cumin) belonging to family Rannunculaceae is an important medicinal plant with worldwide distribution. In Ethiopia, N. sativa occurs in all regions and agro-ecologies at different altitudinal ranges. This plant has a lot of importance in Ethiopia. However, there is no information available on molecular genetic diversity of this crop in respect to Ethiopia. Therefore, the aim of this study was to investigate the genetic diversity of black cumin populations collected from Amhara, Oromia, Tigray, Benshangul and South Nation's Nationality People regions of Ethiopia using ISSR marker. A total of 84 black cumin accessions were obtained from Institute of Biodiversity Conservation (IBC). The ISSR marker was used for computing gene diversity, percent polymorphism, Shannon diversity index and AMOVA. Overall, accessions from Oromia showed the highest gene diversity (H = 0.35) and Shannon information index (I= 0.52), followed by Amhara with gene diversity and Shannon index values of (0.35) and (0.51), respectively. NJ and UPGMA results showed strong grouping among accessions collected from the Oromia and Amhara region. The five geographical regions of Ethiopia showed different levels of genetic variation. Thus, conservation priority should be given for those regions that have low genetic diversity.

Genetic structure and diversity of Adonis L. (Ranunculaceae) populations collected from Turkey by inter-primer binding site (iPBS) retrotransposon markers

“…Dichogamy function is supported by different (biological and morphological) mechanisms, and in this way, cross-fertilization is provided in this family, which increases genetic diversity (Denisow et al, 2014) within the population. Similar findings were reported for other plants, such as A. vernalis (Hirsch et al, 2015), A. aestivalis (Meyer et al, 2015), Ajowan (Heidari et al, 2016), Nigella sativa (Birhanu et al, 2015), and Falcaria vulgaris (Piya et al, 2014).…”

Genetic diversity analysis of cultivated Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] populations from southwestern Ethiopia using inter simple sequence repeats (ISSR) marker

“…The mean Nei’s genetic diversity (GD) and Shannon’s information index (I) values for all primers were 0.34 and 0.51 respectively, indicating an immense genetic diversity at species level. This result is in accordance with the result obtained in other perennial plants in the Zingiberaceae family like Curcuma alismatifolia from Thailand (GD: 0.29; I: 0.43) [28] and in the spice plant black cumin ( Nigella sativa L.) (GD: 0.42; I: 0.54) [29] from Ethiopia, that have been recently studied with ISSR markers. The values of Nei’s genetic diversity (GD) obtained for native plants of Ethiopia like enset (GD: 0.27) [30], teff (GD: 0.27) [31] and sorgum (GD: 0.21) [32] using ISSR markers in the previous studies showed a lower value than obtained in the present study.…”