Molecular diversity of the equine caecal microbiota and its correlation to postprandial fermentation metabolites: A preliminary approach

Hansen, Naja C Kærsgaard; Mydland, Liv Torunn; Næsset, J. A.; Austbø, D.; Måge, Ingrid; Rudi, Knut

doi:10.1080/09064702.2013.877520

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…This may be explained by its association with fermentation and CO 2 fixation genes, which is consistent with the observation that this family is one of the main SCFA producers in the gut (11, 24). Moreover, species belonging to this family are known to harvest energy by the assimilation of CO 2 and hydrogen (31), which are major end products of rapid fermentation, as observed for horses fed the BARLEY diet.…”

Section: Discussionsupporting

confidence: 88%

Diet-Dependent Modular Dynamic Interactions of the Equine Cecal Microbiota

et al. 2016

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Knowledge on dynamic interactions in microbiota is pivotal for understanding the role of bacteria in the gut. We herein present comprehensive dynamic models of the horse cecal microbiota, which include short-chained fatty acids, carbohydrate metabolic networks, and taxonomy. Dynamic models were derived from time-series data in a crossover experiment in which four cecum-cannulated horses were fed a starch-rich diet of hay supplemented with barley (starch intake 2 g kg−1 body weight per day) and a fiber-rich diet of only hay. Cecal contents were sampled via the cannula each h for 24 h for both diets. We observed marked differences in the microbial dynamic interaction patterns for Fibrobacter succinogenes, Lachnospiraceae, Streptococcus, Treponema, Anaerostipes, and Anaerovibrio between the two diet groups. Fluctuations and microbiota interactions were the most pronounced for the starch rich diet, with Streptococcus spp. and Anaerovibrio spp. showing the largest fluctuations. Shotgun metagenome sequencing revealed that diet differences may be explained by modular switches in metabolic cross-feeding between microbial consortia in which fermentation is linked to sugar alcohols and amino sugars for the starch-rich diet and monosaccharides for the fiber-rich diet. In conclusion, diet may not only affect the composition of the cecal microbiota, but also dynamic interactions and metabolic cross-feeding.

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Section: Discussionsupporting

confidence: 88%

Diet-Dependent Modular Dynamic Interactions of the Equine Cecal Microbiota

et al. 2016

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“…The GI microbiota is crucial for efficient nutrient utilisation in equines, since up to 60% of the forage is digested by microbial fermentation – mostly occurring in the hindgut ( 4 , 5 ). The equine caecum is an enlargement of the hindgut, containing a highly complex microbial ecosystem mainly dominated by members of the bacterial phyla Firmicutes and Bacteroides ( 6 , 7 ).…”

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confidence: 99%

High nutrient availability reduces the diversity and stability of the equine caecal microbiota

Hansen¹,

Avershina²,

Mydland³

et al. 2015

Microbial Ecology in Health & Disease

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BackgroundIt is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.MethodsHere we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.ResultsWe found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.ConclusionNutrient availability does not only change the composition but also the ecology of the caecal microbiota.

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