Molecular diversity of skin mucus lectins in fish

Suzuki, Yuzuru; Tasumi, Satoshi; Tsutsui, Shigeyuki; Okamoto, Masaki; Suetake, Hiroaki

doi:10.1016/s1096-4959(03)00178-7

Cited by 106 publications

(71 citation statements)

References 67 publications

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“…These domains are conserved among mannose-specific lectins of monocots (57,65). The in silico comparative analysis presented in this work, together with other data (11,61), clearly establishes that MMBL domains have a much wider distribution than previously assumed. In this study, we identified MMBL domains not only in various (putative) proteins encoded by fungi and fish genomes but also in phylogenetically unrelated bacteria such as C. violaceum and several actinomycetes.…”

Section: Discussionsupporting

confidence: 68%

Novel Lectin-Like Bacteriocins of Biocontrol Strain Pseudomonas fluorescens Pf-5

Parret

Temmerman

Mot

2005

Appl Environ Microbiol

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Bacteria living in a competitive environment are able to secrete proteinaceous toxins, known as bacteriocins, which can kill closely related bacterial competitors while causing little harm to the bacteriocinogenic cells. These bacterial inhibitors are produced by all major groups of Bacteria (53). Bacteriocin production has also been reported for Halobacteriaceae, a family of extremely halophilic Archaea (36). Bacteriocins constitute a structurally and functionally diverse group within the antimicrobials, ranging from small peptides, such as microcins of Enterobacteriaceae and antibiotics secreted by low-GC-content gram-positive bacteria (23, 31), to middle-sized polypeptides, such as colicins of Escherichia coli (34) and their counterparts in Pseudomonas aeruginosa (S pyocins) (39), to large phage tail-like multiprotein complexes, such as syringacin produced by Pseudomonas syringae (58) and R-and F-type pyocins of P. aeruginosa (39). Bacteriocin mode of killing can be either by membrane pore formation, nonspecific degradation of cellular DNA, cleavage of 16S rRNA or tRNA, or inhibition of peptidoglycan synthesis resulting in cell lysis (51).Bacteriocins from lactic acid bacteria, such as nisin, have received considerable attention, given their potential as food preservatives (41, 42). Among bacteriocins of gram-negative bacteria, colicins are the most intensively studied. Colicins are produced by, and attack, strains of E. coli. These proteins, as well as the S pyocins, are organized in functional domains, namely, regions for cell attachment, translocation, and bactericidal activity. Colicins and S pyocins parasitize specific membrane-bound receptors on target cells, resulting in a rather narrow spectrum of activity. The host strain is protected from its own bacteriocin through the action of a cognate immunity protein that is coproduced with the bacteriocin (39, 51).It has been proposed that bacteriocins may play a key role in bacterial population dynamics (52,53). In a recent study, Kirkup and Riley demonstrated that the production of colicins by E. coli colonizing the mouse colon gives the colicinogenic strains a competitive advantage by killing colicin-sensitive E. coli sharing the same ecological niche (33). Possible applications of bacteriocinogenic strains in agriculture include their use in biological control of soilborne or phyllosphere-inhabiting bacterial plant pathogens. In this way, heterologous production of the peptide bacteriocin trifolitoxin by an avirulent Agrobacterium strain effectively enhances biological control of Agrobacterium vitis crown gall (24). Expression of the trifolitoxin genes from Rhizobium leguminosarum bv. trifolii T24 in Rhizobium etli CE3 increases bean nodulation competitiveness of the recombinant strain in the presence of indigenous rhizobia under agricultural conditions (54). A phage tail-like bacteriocin (serracin P) produced by Serratia plymithicum may be used for biological control of fire blight caused by Erwinia amylovora (30), while Xanthomonas campestris pv. glycin...

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Section: Discussionsupporting

confidence: 68%

Novel Lectin-Like Bacteriocins of Biocontrol Strain Pseudomonas fluorescens Pf-5

Parret

Temmerman

Mot

2005

Appl Environ Microbiol

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“…Furthermore, a diversity of lectins was reported in fish skin mucus [55]. On one hand, in our study, a C-type lectin (spot 1) was identified as over-expressed in both overcrowding stressed fish, reaching 2.61-fold in the group fed with probiotics under stress conditions, compared to the control group (Table 3; Figure 1 D).…”

Section: Accepted M Manuscriptsupporting

confidence: 53%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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“…In addition, pufflectin was found to bind to a parasitic trematode (Heterobothrium okamotoi). Taken together, these results demonstrate that skin mucus lectins in fishes have wide molecular diversity [200]. In another study, a piscine lily-type lectin was described in pufferfish and it was expressed exclusively in mucosal tissues, namely, skin, digestive tract, oral cavity, and gills [201].…”

Section: Lectinsmentioning

confidence: 60%

An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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Molecular diversity of skin mucus lectins in fish

Cited by 106 publications

References 67 publications

Novel Lectin-Like Bacteriocins of Biocontrol Strain Pseudomonas fluorescens Pf-5

Novel Lectin-Like Bacteriocins of Biocontrol Strain Pseudomonas fluorescens Pf-5

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

An Overview of the Immunological Defenses in Fish Skin

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