Molecular diversity of phytoplankton in the East China Sea around Jeju Island (Korea), unraveled by pyrosequencing

Faria, Daphne Georgina; Lee, Man-Duck; Lee, Joon-Baek; Lee, Juyun; Chang, Man; Youn, Seok Hyun; Suh, Young Sang; Ki, Jang‐Seu

doi:10.1007/s10872-013-0208-2

Cited by 25 publications

(6 citation statements)

References 41 publications

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“…It is likely, however, that the maximum temperatures experienced during this experiment (approx. 19–21°C) fell below thermal thresholds for most taxa, many of which are widely distributed and occur in warmer waters [ 41 – 43 ], and experience temperature maxima of a similar magnitude during the summer months within the study region [ 44 , 45 ]. As such, many taxa are likely to have responded positively to higher temperatures, and exhibited faster growth and development on warmed surfaces.…”

Section: Discussionmentioning

confidence: 99%

Community responses to seawater warming are conserved across diverse biological groupings and taxonomic resolutions

et al. 2017

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Temperature variability is a major driver of ecological pattern, with recent changes in average and extreme temperatures having significant impacts on populations, communities and ecosystems. In the marine realm, very few experiments have manipulated temperature in situ, and current understanding of temperature effects on community dynamics is limited. We developed new technology for precise seawater temperature control to examine warming effects on communities of bacteria, microbial eukaryotes (protists) and metazoans. Despite highly contrasting phylogenies, size spectra and diversity levels, the three community types responded similarly to seawater warming treatments of +3°C and +5°C, highlighting the critical and overarching importance of temperature in structuring communities. Temperature effects were detectable at coarse taxonomic resolutions and many taxa responded positively to warming, leading to increased abundances at the community-level. Novel field-based experimental approaches are essential to improve mechanistic understanding of how ocean warming will alter the structure and functioning of diverse marine communities.

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Section: Discussionmentioning

confidence: 99%

Community responses to seawater warming are conserved across diverse biological groupings and taxonomic resolutions

et al. 2017

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“…Skeletonema marinoi is a ubiquitous diatom species that often forms massive blooms in many temperate coastal oceans, such as the Gullmar Fjord, Skagerrak-Kattegat, Baltic Sea, East China Sea, and Northern Adriatic Sea (Mediterranean Sea) (22)(23)(24)(25)(26). Previous studies on S. marinoi have focused more on its physiological responses to nutrient limitation (27), secondary metabolite production (28), biotic interactions with grazers (23,24,29), potential stress/cell death markers (30,31), and responses to silicate limitation and sterol sulfates (StS) with PCD (14,18).…”

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confidence: 99%

Responses of Marine Diatom Skeletonema marinoi to Nutrient Deficiency: Programmed Cell Death

Wang

Chen

et al. 2020

Appl Environ Microbiol

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Diatoms are important phytoplankton and contribute greatly to the primary productivity of marine ecosystems. Despite the ecological significance of diatoms and the importance of programmed cell death (PCD) in the fluctuation of diatom populations, little is known about the molecular mechanisms of PCD triggered by different nutrient stresses. Here we describe the physiological, morphological, biochemical, and molecular changes in response to low levels of nutrients in the ubiquitous diatom Skeletonema marinoi. The levels of gene expression involved in oxidation resistance and PCD strongly increased upon nitrogen (N) or phosphorus (P) starvation. The enzymatic activity of caspase 3-like protein also increased. Differences in mRNA levels and protein activities were observed between the low-N and low-P treatments, suggesting that PCD could have a differential response to different nutrient stresses. When cultures were replete with N or P, the growth inhibition stopped. Meanwhile, the enzymatic activity of caspase 3-like protein and the number of cells with damaged membranes decreased. These results suggest that PCD is an important cell fate decision mechanism in the marine diatom S. marinoi. Our results provide important insight into how diatoms adjust phenotypic and genotypic features of their cell-regulated death programs when stressed by nutrient limitations. Overall, this study could allow us to better understand the molecular mechanism behind the formation and termination of diatom blooms in the marine environment. IMPORTANCE Our study showed how the ubiquitous diatom S. marinoi responded to different nutrient limitations with PCD in terms of physiological, morphological, biochemical, and molecular characteristics. Some PCD-related genes (PDCD4, GOX, and HSP90) induced by N deficiency were relatively upregulated compared to those induced by P deficiency. In contrast, the expression of the TSG101 gene in S. marinoi showed a clear and constant increase during P limitation compared to N limitation. These findings suggest that PCD is a complex mechanism involving several different proteins. The systematic mRNA level investigations provide new insight into understanding the oxidative stress- and cell death-related functional genes of diatoms involved in the response to nutrient fluctuations (N or P stress) in the marine environment.

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“…Total genomic DNA was extracted by the modified method by Faria et al . (2014). A 2‐mL microcentrifuge tube containing the harvested cells was subjected to freeze–thaw cycles using liquid N 2 and a 65°C water bath.…”

Section: Methodsmentioning

confidence: 99%

Edaphophycus epilithus gen. et sp. nov. (Oscillatoriales, cyanobacteria) with a description of the morphology and molecular phylogeny

Lee

Kim

et al. 2022

Phycological Research

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Two strains of subaerial cyanobacteria were collected from two locations of the Republic of Korea, one in Seoul and the other in Gyeonggi-do, both of which were studied using morphological and molecular traits. The genus Edaphophycus belonging to the family Coleofasciculaceae is morphologically very similar to the type genus Coleofasciculus and genus Kastovskya. However, in genera Coleofasciculus and Roseofilum thylakoids were reported as radial arrangements, whereas in genera Anagnostidinema, Geitlerinema, Kastovskya, and Wilmottia, belonging to family Coleofasciculaceae, thylakoids were reported as parietal arrangements, and in genus Edaphophycus in this study, thylakoids also appeared as parietal arrangements. Phylogenetic analysis of 16S rRNA showed that our two Edaphophycus epilithus strains clustered in the same clade, forming a sister relationship with genus Anagnostidinema. This suggested that our species belongs to the family Coleofasciculaceae. In addition, the ITS structure of E. epilithus showed different features when compared with genera Coleofasciculus and Anagnostidinema. These results demonstrated that E. epilithus sp. nov. is unique in morphology and molecular traits. Therefore, we propose this to be a novel species belonging to family Coleofasciculaceae and genus Edaphophycus gen. nov.

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Molecular diversity of phytoplankton in the East China Sea around Jeju Island (Korea), unraveled by pyrosequencing

Cited by 25 publications

References 41 publications

Community responses to seawater warming are conserved across diverse biological groupings and taxonomic resolutions

Community responses to seawater warming are conserved across diverse biological groupings and taxonomic resolutions

Responses of Marine Diatom Skeletonema marinoi to Nutrient Deficiency: Programmed Cell Death

Edaphophycus epilithus gen. et sp. nov. (Oscillatoriales, cyanobacteria) with a description of the morphology and molecular phylogeny

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