Molecular diversity of phosphate solubilizing bacteria isolated from the rhizosphere of chickpea, mustard and wheat

Solanki, Manu; Kundu, B. S.; Nehra, Kiran

doi:10.1016/j.aasci.2018.05.007

Cited by 16 publications

(7 citation statements)

References 26 publications

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“…Bacillus subtilis has shown maximum ability for phosphate solubilization as studied by Ahmad et al (2018). Phosphate solubilizing bacteria improve phosphate availability to plants since in acidic and basic soil, phosphate available is reduced in the plant roots due to strong bonding with calcium and magnesium but bacterial isolates secrete certain enzymes that disrupt this linkage and help to improve the phosphate availability to the plants (Solanki, Kundu & Nehra, 2018). Such bacteria that have high phosphate solubilization efficiency to help plant grow in salt-affected soil.…”

Section: Discussionmentioning

confidence: 99%

Bacillus spp.: potent microfactories of bacterial IAA

Wagi

Ahmed

2019

PeerJ

113

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Background Auxin production by bacteria is one of the most important direct mechanisms utilized by plant growth-promoting bacteria (PGPB) for the betterment of plants naturally because auxin is a plant friendly secondary metabolite synthesized naturally by bacteria, and hence improves the growth of associated plants. So, the current study focuses on bacterial synthesis of Indole-3-acetic acid (IAA) for plant growth improvement. Methods In the current study, the PGPB were selected on the basis of their auxin production potential and their growth promoting attributes were evaluated. Indole-3-acetic acid producing potential of two selected bacterial isolates was observed by varying different growth conditions i.e., media composition, carbon sources (glucose, sucrose and lactose) and different concentrations of precursor. Influence of various physiological factors (temperature and incubation time period) on IAA production potential was also evaluated. Results Both the bacterial strains Bacillus cereus (So3II) and B. subtilis (Mt3b) showed variable potential for the production of bacterial IAA under different set of growth and environmental conditions. Hence, the IAA production potential of the bacterial isolates can be enhanced by affecting optimum growth conditions for bacterial isolates and can be used for the optimal production of bacterial IAA and its utilization for plant growth improvement can lead to better yield in an eco-friendly manner.

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Section: Discussionmentioning

confidence: 99%

Bacillus spp.: potent microfactories of bacterial IAA

Wagi

Ahmed

2019

PeerJ

113

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“…Chemical fertilizers are a way to provide P to crops. However, its availability remains low due to bond formation with Fe 3+ and Al 3+ in acid soils and with Ca 2+ and Mg +2 in alkaline soils (Solankia et al (2018); Wei et al (2018)). Most phosphate chemical fertilizers are obtained from phosphate rock.…”

Section: Introductionmentioning

confidence: 99%

“…PSB solubilize inorganic phosphate through different mechanisms via organic acid production and mineralize organic phosphate through the production of phosphatases (Beheshti et al (2017); Munda et al (2018); Tahir et al (2018); Zhu et al (2018)). Solubilization and mineralization processes mobilize the applied P and accumulate it in the soil, reducing fertilizer application costs (Behera et al (2017)), improving the soil's quality, and re-establishing ecological equilibrium (Solankia et al (2018)).…”

Section: Introductionmentioning

confidence: 99%

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

et al. 2021

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Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 g L-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse- and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12 h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5 g L-1) fourfold lower than those in SMRS1 (10.0 g L-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08/ log10 CFUmL-1 and, at 6 h of culture, acid and alkaline phosphatase enzyme volumetric activities of 2.3 ± 0.8 and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17) mg L-1 at 6 h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1) of bioinoculant plus 100 kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables (170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus (p < 0.05).

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“…It has been reported that higher concentrations of phosphate-solubilizing bacteria are commonly found in the rhizosphere soil as compared to the non-rhizospheric soil (Reyes et al,2012) and the secretion of organic acid in the rhizosphere area leads to solubilization of insoluble phosphate and make it available to the plants (Richardson et al, 2009). Phosphate solubilizing diversity of endophytic bacteria was less compared to the rhizosphere area (Patel et al, 2017;Solanki et al, 2018). All the isolates were negative for the HCN production, whereas all isolates positive for protease activity.…”

Section: Discussionmentioning

confidence: 99%

Root Associated Bacterial Endophytes From Poaceae Plants: Identification, Characterization and Plant Growth Promotion

Patel¹,

Agrawal²,

Sidhdhapara³

2020

J microb biotech food sci

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Diazotrophic endophytic bacteria contribute to the plant growth using various plant growth promoting traits as well as by inducing the plant defence mechanism upon pathogen attack. Aim of the present study was to isolate true diazotrophic endophytic bacteria from the total diazotrophic bacterial community of wheat and pearl-millet plants. Further, these isolates were evaluated for their potential of plant growth promotion by in vitro and in vivo experiments. We isolated total three diazotrophic endophytic bacteria based on their growth after repetitive subculturing on nitrogen free medium. Molecular characterization of all three isolates showed affiliation to the genera Agrobacterium, Pseudomonas and Brevundimonas. In vitro analysis of plant growth promoting traits showed positive result for indole acetic acid (IAA) production, cellulase and protease activity. The IAA production is found between the ranges of 98 to 113 µg ml-1. All the endophytic bacterial isolates showed the PCR amplification of NifH gene and ammonia production. Diazotrophic endophytic bacterial isolates (PR1, JB3 and JB6) were tested for their capacity to promote the plant growth using pot experiment. All the diazotrophic endophytic bacteria differ in their plant growth promotion ability as analyzed by various plant growth parameter compared to the untreated control plants. In Pot experiment, PR1 and JB6 showed a significant difference in dry weight of root, whereas JB3 and JB6 showed the significant differences in shoot length and total nitrogen content (p≤0.05) compared to the untreated plants.

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Molecular diversity of phosphate solubilizing bacteria isolated from the rhizosphere of chickpea, mustard and wheat

Cited by 16 publications

References 26 publications

Bacillus spp.: potent microfactories of bacterial IAA

Bacillus spp.: potent microfactories of bacterial IAA

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

Root Associated Bacterial Endophytes From Poaceae Plants: Identification, Characterization and Plant Growth Promotion

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