Molecular diversity in castor (Ricinus communis L.)

Kallamadi, Prathap Reddy; Nadigatla, V.P.R. Ganga Rao; Sujatha, M.

doi:10.1016/j.indcrop.2014.12.061

Cited by 44 publications

(23 citation statements)

References 36 publications

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“…In the case of R. communis, ISSRs have been used recently to assess the genetic diversity of genotypes from India (Gajera et al 2010;Kulshrestha and Talati 2014;Tomar Rukam et al 2014), China (Wang et al 2013) and other countries (Kallamadi et al 2015). In all cases, dendrograms have been produced revealing the genetic closeness of the selected genotypes.…”

Section: Resultsmentioning

confidence: 99%

Dwarf hybrids of the bioenergy crop Ricinus communis suitable for mechanized harvesting reveal differences in morpho-physiological characteristics and seed metabolic profiles

et al. 2016

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Ricinus communis, also known as castor bean or castor plant, is a crop that produces seeds containing non-edible oil used widely in the chemical industry and the bioenergy sector. Varieties or hybrids that are suitable for mechanized harvesting may diminish the production cost boosting large scale production of the crop in new areas. In this study, we describe extensively the morphological characteristics and the ex situ performance of four newly developed R. communis hybrids (H11, H12, H13, and H14) focusing on plant architecture in order to facilitate mechanized harvesting. In addition, hybrid germination rate at low and optimal temperature was evaluated aiming to determine a minimal temperature requirement for early spring sowing in temperate climates. Seed metabolic profiling using LC-MS/MS analysis revealed the presence of more than 60 different primary and secondary metabolites, at varied levels in each hybrid, including amino acids, fatty acids, flavonols, flavones, flavanones, phenyl propanoids and catecholamines such as dopamine, many of which are reported for the first time in this species.

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Section: Resultsmentioning

confidence: 99%

Dwarf hybrids of the bioenergy crop Ricinus communis suitable for mechanized harvesting reveal differences in morpho-physiological characteristics and seed metabolic profiles

et al. 2016

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“…Besides, TRAP markers optimize the genetic gains in genetic improvement programs and are a valuable tool used by these programs to study genetic divergence (Agarwal et al, 2008). Different molecular markers have been used to estimate the degree of genetic divergence among genotypes of castor bean, for example, AFLP and simple sequence repeats (SSR) (Pecina-Quintero et al, 2013;Tan et al, 2013;Gálová et al, 2015;Machado et al, 2016), and RAPD and inter-simple sequence repeats (ISSR) (Silva et al, 2012;Tomar et al, 2014;Lakhani et al, 2015;Vivodík et al, 2015;Goodarzi et al, 2015;Kallamadi et al, 2015). However, functional TRAP markers have never been used to evaluate the genetic divergence in castor bean.…”

Section: Introductionmentioning

confidence: 99%

Research Article Genetic divergence in elite castor bean lineages based on TRAP markers.

et al. 2017

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ABSTRACT. Castor bean (Ricinus communis L.) is a tropical plant of great commercial interest and a potential source of biodiesel. The development of genetically improved cultivars with high amounts of oil in the seeds and low ricin toxicity is crucial to increase the productivity of this crop. The use of TRAP (target region amplification polymorphism) markers to develop elite lineages and study genetic divergence is fundamental to advance the genetic improvement of this species. The goal of this study was to evaluate the genetic divergence among 40 elite lineages of R. communis, which belong to the NBIO-UFRB Genetic Improvement Program, using TRAP markers involved in the biosynthesis of oil and ricin. Total DNA was extracted and quantified from the leaf tissue of the castor bean plants, and 70 TRAP combinations (fixed and arbitrary primers) were used to genotype the 40 lineages. Of the 580 fragments amplified, 335 were polymorphic (58%). The genetic dissimilarity among the lineages was calculated by the Jaccard dissimilarity index using the UPGMA grouping method. A dendrogram was generated, and four groups formed, showing divergence among the elite lineages that favors selection. The TRAP 2 K.S. Simões et al. Genetics and Molecular Research 16 (3): gmr16039776 molecular markers were efficient at characterizing the genetic variability among the lineages and, because TRAP markers are functional markers involved in the biosynthesis of oil and ricin, they are important when studying the association between a marker and a candidate gene.

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“…Different types of molecular markers have been used to study the genetic variability of castor bean: amplified fragment length polymorphism (AFLP) (Pecina-Quintero et al, 2013), random amplified DNA polymorphism (RAPD) (Gajera et al, 2010;Silva et al, 2012;Vivodík et al, 2014Vivodík et al, , 2015Lakhani et al, 2015), single nucleotide polymorphism (Foster et al, 2010), simple sequence repeats (SSR) (Bajay et al, 2009(Bajay et al, , 2011Seo et al, 2011;Tan et al, 2013Tan et al, , 2014Gálová et al, 2015;Machado et al, 2016), and inter-simple sequence repeats (ISSR) Tomar et al, 2014;Goodarzi et al, 2015;Kallamadi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of TRAP primers for Ricinus communis L.

et al. 2017

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ABSTRACT. The objective of this article was to develop TRAP (target region amplification polymorphism) primers for castor bean, with the goal of making functional markers available for genetic studies about the species. To do this, oligonucleotides were designed based on ESTs, obtained from the NCBI (National Center for Biotechnology Information) databank, which code enzymes involved in metabolic routes of fatty acid synthesis, ricin synthesis, and resistance to castor bean pathogens. The forward primers were designed with the help of the Primer3 software and, for the reverse, six arbitrary primers were used. To standardize the amplification reactions, the following criteria were used to select the primers: sizes between 18 and 20 bp, guanine/ cytosine (GC) in the range of 40 to 60%, and average annealing temperature between 55° and 62°C. The design quality of the primers was verified using the Net Primer application. Fifty-six primers were designed, which had an average GC percentage of 53.2%. A total of 336 combinations were obtained using the 56 fixed and 6 arbitrary primers. Based on polymerase chain reaction, 330 combinations (89%) presented good amplification patterns for the genomic DNA of castor bean. The size of the fragments amplified varied between 50 and 2072 bp. The TRAP primers designed and validated in this study are the first for castor bean and represent a significant increase in the molecular markers for this species.

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Molecular diversity in castor (Ricinus communis L.)

Cited by 44 publications

References 36 publications

Dwarf hybrids of the bioenergy crop Ricinus communis suitable for mechanized harvesting reveal differences in morpho-physiological characteristics and seed metabolic profiles

Dwarf hybrids of the bioenergy crop Ricinus communis suitable for mechanized harvesting reveal differences in morpho-physiological characteristics and seed metabolic profiles

Research Article Genetic divergence in elite castor bean lineages based on TRAP markers.

Development of TRAP primers for Ricinus communis L.

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