Molecular Diversity and Genetic Structure of Guineagrass (Panicum maximum Jacq.), a Tropical Pasture Grass

Sousa, Adna Cristina Barbosa de; Jank, Liana; Campos, Tatiana de; Sforça, Danilo Augusto; Zucchi, Maria Imaculada; Souza, Anete Pereira de

doi:10.1007/s12042-011-9081-6

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…The two-and three-dimensional graphs of accession differentiation were revealed by principal component analysis. This type of graphical illustration enables an assessment of the population structure and geometric distances among all of the accessions in the study (de Sousa et al, 2011). These groupings were entirely consistent with those of the clustering results.…”

Section: Discussionsupporting

confidence: 80%

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers

Yuan¹,

Zhang²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Okra (Abelmoschus esculentus L.) is not only a nutrientrich vegetable but also an important medicinal herb. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 24 okra genotypes. In this study, the PCR products were separated by electrophoresis on 8% nondenaturing polyacrylamide gel and visualized by silver staining. The 22 ISSR primers produced 289 amplified DNA fragments, and 145 (50%) fragments were polymorphic. The 289 markers were used to construct the dendrogram based on the unweighted pair-group method with arithmetic average (UPGMA) cluster analysis. The dendrogram indicated that 24 okras were clustered into 4 geographically distinct groups. The average polymorphism information content (PIC) was 0.531929, which showed that the majority of primers were informative. The high values of allele frequency, genetic diversity, and heterozygosity showed that primer-sample combinations produced measurable fragments. The mean distances ranged from 0.045455 to

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Section: Discussionsupporting

confidence: 80%

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers

Yuan¹,

Zhang²,

Wang³

et al. 2014

Genet. Mol. Res.

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“…Species such as Panicum virgatum are being intensely studied for energy production [11]–[13]. Currently, the breeding of tropical forage grasses such as P. maximum and several Brachiaria species is based primarily on the assessment and selection of natural genotypes represented in germplasm banks, using the variability obtained from grasses of African origin [14], [15]. The development of more productive and efficient cultivars can be improved through the use of genomics, transcriptomics and proteomics.…”

Section: Introductionmentioning

confidence: 99%

“…The characterization of molecular markers is important for marker-assisted selection, germplasm assessment, the identification of hybrids and genome mapping [3]. Studies focusing on the molecular biology of P. maximum , including the characterization of molecular markers, genetic profiling, the search for apomixis-related genes and genetic evaluation of germplasm collections, have been performed [7], [15]–[20]. However, knowledge about the Guinea grass transcriptome remains limited, and very few P. maximum protein and nucleotide sequences are available in current databases.…”

Section: Introductionmentioning

confidence: 99%

De Novo Transcriptome Assembly for the Tropical Grass Panicum maximum Jacq

Toledo-Silva

Cardoso-Silva

Jank³

et al. 2013

PLoS ONE

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Guinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using high-throughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses.

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“…Some examples are Ranunculus carpaticola (Paun et al, 2006), Brachiaria humidicola ( Jungmann et al, 2010), Paspalum notatum (Espinoza et al, 2006;Cidade et al, 2008;Reyno et al, 2012), and Panicum maximum (Sousa et al, 2011). Some examples are Ranunculus carpaticola (Paun et al, 2006), Brachiaria humidicola ( Jungmann et al, 2010), Paspalum notatum (Espinoza et al, 2006;Cidade et al, 2008;Reyno et al, 2012), and Panicum maximum (Sousa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Although apomixis is responsible for the creation of genetically uniform populations, apomictic species usually contain a rich diversity. Some examples are Ranunculus carpaticola (Paun et al, 2006), Brachiaria humidicola ( Jungmann et al, 2010), Paspalum notatum (Espinoza et al, 2006;Cidade et al, 2008;Reyno et al, 2012), and Panicum maximum (Sousa et al, 2011). Little is known about how this diversity is generated in apomictic species and how polyploidy and apomixis contribute to the adaptation of these species to different environments.…”

mentioning

confidence: 99%

Diversity in Apomictic Populations of Paspalum simplex Morong

et al. 2014

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Paspalum is considered a genetic model for studying the sources of genotypic variability and breeding techniques in apomictic plants. Paspalum simplex is a warm‐season forage grass that well represents the genus since it contains different ploidy levels and apomixis is linked to polyploidy. The objective was to evaluate the diversity present within and among apomictic polyploid populations of P. simplex. Germplasm was collected from 17 sites covering the species’ region of natural distribution. The diversity present at the molecular level within and among populations was evaluated using ISSR markers. Variability for agronomic traits was also evaluated by cultivating all populations into the field. The 17 analyzed populations were all polyploid, including 13 pure tetraploid, and 4 mixed tetraploid‐hexaploid with predominance of the tetraploid cytotype. Most of the diversity was present among polyploid populations (85% of the total variation), and there was not a correlation between genetic and geographical distances. The within‐population diversity was low for most populations with the exception of one of them. Each genotype was restricted to a single location. Variability for initial growth, spring and fall growth, and the extent of the vegetative phase was observed within and among polyploid populations. The within‐population variation for these phenotypic traits was mainly due to the presence of one or a few off‐type plants. A highly genotype‐specific colonization of new sites appears to occur in P. simplex, and then apomixis mediates the formation of uniform populations.

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Molecular Diversity and Genetic Structure of Guineagrass (Panicum maximum Jacq.), a Tropical Pasture Grass

Cited by 13 publications

References 27 publications

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers

De Novo Transcriptome Assembly for the Tropical Grass Panicum maximum Jacq

Diversity in Apomictic Populations of Paspalum simplex Morong

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