Molecular Divergence Studies through RAPD Markers in Potato  (Solanum tuberosum L.) Germplasm

Verma, Anamika; Singh, Dhirendra Kumar

doi:10.20546/ijcmas.2019.806.293

Cited by 1 publication

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“…They studied a smaller number of primers which may be cause of scoring less size range bands. Moreover, Verma and Singh [1] reported 91 bands while screening 48 Indian potato by 20 RAPD markers i.e. 4.55 bands per primer and 1.9 bands per potato cultivar.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Diversity Analysis of Somaclonal Variants of Potato (Solanum tuberosum L.) by Random Amplified Polymorphic DNA Markers

Shamsuzzaman

Bhattacharjya

Islam

et al. 2021

ARRB

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Aims: An experiment was conducted to analyze the DNA fingerprinting and genetic diversity of nine potato (Solanum tuberosum L.) somaclonal variants and three check varieties. Place and Duration of Study: The experiment was carried out at the Biotechnology laboratory of the Department of Biotechnology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh during November, 2013 to December, 2014. Methodology: The somaclonal variants investigated were SIP-3, SIP-5, SVP-6, SVP-18, SVP-19, SVP-25, SVP-55, SVP-56 and SVP-68, while the check varieties were Cardinal, Diamant and Asterix. Six RAPD primers were used to perform PCR reaction after genomic DNA was extracted from young leaves. Results: The selected 6 primers produced total 54 distinct and differential amplified DNA bands of size range 88 bp to 3265 bp, where 47 bands (~87%) were polymorphic and 7 bands (~13%) were monomorphic. The pair-wise inter-genotype similarity indices were ranged from 61.59% to 93.55% with an average of 74.31%. The Nei’s genetic distance among 12 potato genotypes was estimated from 0.0972 to 0.6217 whereas, genetic identity was between 0.5370 and 0.9074. Here, the distantly linked accessions among the somaclonal variations with check varieties were SVP-6 (to Cardinal and Diamant) and SVP-25 (to Asterix). In addition, the UPGMA dendrogram segregated the 12 potato genotypes into two broad clusters containing 8 and 4 genotypes, respectively. Furthermore, the dendrogram also displayed the highest genetic distance between SVP-6 vs SVP-68 genotype pair. Conclusion: Significant relationship and diversity were found among the studied 12 genotypes. This genetic diversity among the potato genotypes would be utilized for further potato improvement.

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Section: Discussionmentioning

confidence: 99%

“…Potato (Solanum tuberosum L.) is one of the most important members of family Solanaceae and genus Solanum [1]. More than 4000 edible potato varieties are cultivated throughout the world whereas, 27 local and 90 BARI released potato varieties are grown in Bangladesh [2].…”

Section: Introductionmentioning

confidence: 99%

Molecular Diversity Analysis of Somaclonal Variants of Potato (Solanum tuberosum L.) by Random Amplified Polymorphic DNA Markers

Shamsuzzaman

Bhattacharjya

Islam

et al. 2021

ARRB

View full text Add to dashboard Cite

show abstract

Molecular Divergence Studies through RAPD Markers in Potato (Solanum tuberosum L.) Germplasm

Abstract: Genetic diversity is also used to study the taxonomic relationship among genotypes and to choose varieties with good qualities and incorporate them into breeding programmes (Barandalla et al., 2006).

Cited by 1 publication

References 13 publications

Molecular Diversity Analysis of Somaclonal Variants of Potato (Solanum tuberosum L.) by Random Amplified Polymorphic DNA Markers

Molecular Diversity Analysis of Somaclonal Variants of Potato (Solanum tuberosum L.) by Random Amplified Polymorphic DNA Markers

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