Molecular divergence of Grapevine virus A (GVA) variants associated with Shiraz disease in South Africa

Goszczynski, D. E.; Preez, Jacques Du; Burger, Johan T.

doi:10.1016/j.virusres.2008.08.014

Cited by 26 publications

(46 citation statements)

References 11 publications

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“…Only isolates of group III induced mild symptoms in the experimental host Nicotiana benthamiana (45). These isolates appeared not to induce in grapevine cv Shiraz a serious disorder known in South Africa as Shiraz disease (SD), whereas a variant of group I dominated in SD-affected vines (46). By contrast, one of the three sequenced GRSPaV variants was shown to infect Vitis rupestris without inducing symptoms (84).…”

Section: Gvb: Grapevine Virus Bmentioning

confidence: 92%

“…Plants infected with GVA, GVB, and GRSPaV often harbor a population of viral sequence variants (45,46,83,107), some of which endowed with differential pathogenicity. Thus, the comparative analysis of the 3 -terminal regions of a number of GVA isolates allowed their separation into three molecular groups that share sequence identity within groups above 90% and below this value between the groups.…”

Section: Gvb: Grapevine Virus Bmentioning

confidence: 99%

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FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

319

196

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The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, common features in genome type and organization, and strong phylogenetic relationships between replicational and structural proteins. In this review, we provide a brief account of the main biological and molecular properties of the members of the family, with special emphasis on the relationships within and among the genera. In phylogenetic analyses the potexvirus-like replicases were more closely related to tymoviruses than to carlaviruses. We postulate a common evolutionary ancestor for the family Tymoviridae and the two distinct evolutionary clusters of the Flexiviridae, i.e., a plant virus with a polyadenylated genome, filamentous virions, and a triple gene block of movement proteins. Subsequent recombination and gene loss would then have generated a very diverse group of plant and fungal viruses.

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Section: Gvb: Grapevine Virus Bmentioning

confidence: 92%

Section: Gvb: Grapevine Virus Bmentioning

confidence: 99%

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

319

196

View full text Add to dashboard Cite

show abstract

“…GVB genome sequencing strategy RT-PCR amplification, cloning, and sequencing of the genome of the GVB-H1 variant recovered in N. benthamiana was carried out in three steps according to the standard sequencing strategy reported earlier by Goszczynski et al [11]. In the first step, various primers for RT-PCR amplification of 600-1000 nt sequences covering the whole GVB genome were designed using sequence data from the Italian isolate of GVB [21] deposited in the GenBank/EMBL database with accession number X75448, named here GVB Italy.…”

Section: Isolation Of Dsrnamentioning

confidence: 99%

Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines

Goszczynski

2010

Virus Genes

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Analysis of two Grapevine virus B (GVB)-infected LN33 hybrid grapevines revealed that a plant exhibiting clear symptoms of corky bark (CB) disease was infected with two molecular variants of the virus, whereas a plant exhibiting no disease symptoms was infected with only one variant. Sequence results indicated that the single variant in the CB-negative grapevine was also one of the two present in the CB-affected hybrid. Plant extracts from these two grapevines were used to successfully transmit the virus to N. benthamiana. After further cloning and sequencing, two clearly divergent variants were identified. Comparative molecular analysis of the variants, named here GVB 953-1 and GVB-H1, respectively, transmitted from CB-affected and consistently CB-negative plants, revealed short genomic regions, most of them highly divergent, that encoded amino acid sequences, containing significant amino acid substitutions altering the net charges of their respective proteins. Interestingly, a comparison of these variants to genome sequence data of GVB variants GVB Italy and GVB 94/971 available from the GenBank, revealed that these significant amino acid substitutions were the same for, and unique to, the variant pairs GVB 953-1/GVB Italy and GVB-H1/GVB 94/971. This despite the variants of each pair being otherwise clearly different at nucleotide and amino acid levels. In addition, both sets of variants differed substantially in their respective 3'-non-translated (3'NTR) regions. The relevance of these findings is discussed.

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“…GVA is related with the Kober stem grooving symptom, where severe grooving on the grafted stems occurs [86,87], while GVB is associated with the corky bark syndrome consisting of soft, rubbery and abnormal swelling of the basal internodes of the canes, longitudinal cracks and cork forming, typical of the rugose wood complex [88]. Vitivirus genus has other species less ubiquitous, named GVD, GVE, and the most recently discovered GVF [89], causing similar symptoms to the corky rugose wood but its role is still unclear [90].…”

Section: Grape and Wine Biotechnology 14mentioning

confidence: 99%

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Armijo¹,

Espinoza²,

Loyola³

et al. 2016

Grape and Wine Biotechnology

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Grapevine is one of the most abundant crops worldwide, with varieties destined for fresh and dry consumption, as well as wine production. Unfortunately, grapevine plants are affected by both biotic and abiotic stresses, generating significant economic losses. These conditions can negatively impact grape cultivation at different stages: plant and berry development during pre-and post-harvest, production, fresh fruit processing and export, along with wine quality. Most of the grapevine varieties are susceptible to several pathogens and within this chapter, particular attention is given to fungi (Botrytis cinerea and Erysiphe necator) and viruses, since they are a worldwide concern. Within the latter, special focus is given to the grapevine leafroll disease, a complex and destructive infection. On the other hand, abiotic stress is also relevant in grapevine, and in this chapter it will be exemplified by UV-B radiation and its impact on growth and fruit development, plant adaptive responses and its relationship with the quality of grape berries for winemaking. The main biotic and abiotic grapevine stress factors are reviewed in this chapter, considering a special focus on biotechnological approaches carried out in order to address them and minimize their detrimental consequences.

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Molecular divergence of Grapevine virus A (GVA) variants associated with Shiraz disease in South Africa

Cited by 26 publications

References 11 publications

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

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