Molecular differentiation of Keratinomyces (Trichophyton) species

Guillamón, José Manuel; Cano, Josep; Ramón, Daniel; Guarro, Josep

doi:10.1007/bf00399610

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…Specific yeast strains have been found in association with different grape varieties [29]. Yet, in distant regions, clustering S. cerevisiae strains suggests a restricted geographic gene flow [30,31]. In our study, according to their microsatellite patterns, strains associated to a particular grapevine cultivar or vineyard were not observed.…”

Section: Genetic Diversity Of Autochthonous S Cerevisiae Wine Yeasts ...contrasting

confidence: 55%

Sexually-Driven Combinatorial Diversity in Native Saccharomyces Wine Yeasts

et al. 2022

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Natural diversity represents an inexhaustible source of yeasts for the diversification of wines and the improvement of their properties. In this study, we analysed the genetic diversity of autochthonous Saccharomyces cerevisiae wine yeasts in the Aljarafe of Seville, one of the warmest winemaking regions of Spain. Through multiplex-PCR analysis of five microsatellite markers and RT-PCR determination of the killer genotype, we found 94 different patterns among 150 S. cerevisiae yeast strains isolated from spontaneous fermentation of grape must, thereby representing a highly diverse population. Remarkably, 92% of the isolated strains exhibited high sporulation capacity. Tetrad analysis of sporulating strains rendered a microsatellite marker’s combinatory that mimics patterns observed in the native population, suggesting that the high polymorphism of microsatellite markers found in these wild yeasts might result from sexual reproduction in their natural environment. The identification of unconventional M2/L-A-lus totivirus combinations conferring the killer phenotype also supports this suggestion. One idea behind this study is to determine to what extent the vineyards microbiota in areas with warm climates can provide useful natural yeasts to adapt fermentation processes to the needs imposed by global warming. Analysis of traits of oenological interest in regions potentially affected by global climate changes, such as growth tolerance to ethanol and to sugar stress in the analysed strains, indicated that this broad combinatorial diversity of natural S. cerevisiae yeasts provides a wide range of autochthonous strains with desirable profiles for quality winemaking in warm regions. This combinatorial diversity renders strains with diverse oenological performing abilities. Upon microvinification assays and organoleptic attests, a S. cerevisiae strain with interesting oenological properties has been identified. This result can be considered a successful outcome in industry–academia collaboration.

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Section: Genetic Diversity Of Autochthonous S Cerevisiae Wine Yeasts ...contrasting

confidence: 55%

Sexually-Driven Combinatorial Diversity in Native Saccharomyces Wine Yeasts

et al. 2022

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“…These fungi are mainly classified now by using the taxonomic scheme proposed by Emmons (148), which stress the importance of the conidial morphology and accessory structures. On this basis, the three aforementioned anamorphic genera have been traditionally accepted (3,457), although some authors have considered Keratinomyces (Table 7) to be a fourth genus (118,219). The morphological characteristics used for differentiating some species and genera of dermatophytes, particularly between Epidermophyton and Trichophyton, seem to be inconsistent.…”

Section: Plectomycetesmentioning

confidence: 99%

Developments in Fungal Taxonomy

1999

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SUMMARY Fungal infections, especially those caused by opportunistic species, have become substantially more common in recent decades. Numerous species cause human infections, and several new human pathogens are discovered yearly. This situation has created an increasing interest in fungal taxonomy and has led to the development of new methods and approaches to fungal biosystematics which have promoted important practical advances in identification procedures. However, the significance of some data provided by the new approaches is still unclear, and results drawn from such studies may even increase nomenclatural confusion. Analyses of rRNA and rDNA sequences constitute an important complement of the morphological criteria needed to allow clinical fungi to be more easily identified and placed on a single phylogenetic tree. Most of the pathogenic fungi so far described belong to the kingdom Fungi; two belong to the kingdom Chromista. Within the Fungi, they are distributed in three phyla and in 15 orders (Pneumocystidales, Saccharomycetales, Dothideales, Sordariales, Onygenales, Eurotiales, Hypocreales, Ophiostomatales, Microascales, Tremellales, Poriales, Stereales, Agaricales, Schizophyllales, and Ustilaginales).

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“…Mitochondrial DNA restriction fragment length polymorphism (mtRFLP) analysis has been widely applied to the characterization of reference and commercial Saccharomyces cerevisiae wine yeast strains (118)(119)(120)(121)(122)(123), as well as strains belonging to other species (116,124). Not all the enzymes used in this method detect the same degree of polymorphisms, which depend greatly on the species.…”

Section: Molecular Characterization Of Torulaspora Delbrueckii Strainsmentioning

confidence: 99%