Molecular Differentiation of<i>Treponema pallidum</i>Subspecies

Centurión-Lara, Arturo; Molini, Barbara J.; Godornes, Charmie; Sun, Eileen S.; Hevner, Karin; Voorhis, Wesley C. Van; Lukehart, Sheila A.

doi:10.1128/jcm.00784-06

Cited by 74 publications

(57 citation statements)

References 25 publications

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“…The use of a combination of classical serological testing and cutting-edge molecular techniques such as the quadriplex PCR will help determine the current geographic distribution of yaws, increase the ability to identify the most potentially infectious cases, and detect treatment failures or reinfections following mass treatment with azithromycin. When used in combination with a previously published molecular assay to screen for point mutations associated with azithromycin resistance and/or treatment failures, 8 the quadriplex PCR assay could be used to monitor the possible emergence of macrolide-resistant TP-pertenue strains when azithromycin usage increases as a result of implementation of the WHO's yaws eradication strategy.…”

Section: Discussionmentioning

confidence: 99%

“…The analytic sensitivity of the real-time quadriplex PCR assay was determined using 10-fold serial dilutions of purified TPpertenue genomic DNA (CDC-1 strain). The analytical specificity of the PCR assay was verified using DNA purified from 10 laboratory strains of TP-pallidum 8,9 (Nichols, SS 14, Mexico A, JV1, DAL-1, Madras, 1 strain from Maryland, 3 strains from Minnesota) and 11 clinical strains from South Africa, 11 13 laboratory strains of TP-pertenue 8,9 (CDC-1, CDC-2, CDC-2575, Samoa D, Samoa F, Ghana 051, Gauthier, and 6 strains from Indonesia), a clinical strain from Democratic Republic of the Congo, 6 and 2 strains of TP-endemicum (Bosnia A and Iraq B). In addition, a previously used panel of nonpathogenic treponemes (T. denticola, T. refringens, and T. phagedenis) and other microorganisms was included to determine the specificity of the assay.…”

Section: Methodsmentioning

confidence: 99%

“…However, subspecies-specific genetic signatures permit molecular differentiation using methods that involve polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and DNA sequencing. 3,[6][7][8][9] These existing molecular methods are inherently technically complex resulting in inevitable delays in reporting results. We therefore developed a TaqMan-based real-time quadriplex PCR assay to rapidly and simultaneously differentiate between TP-pallidum, TPpertenue, and TP-endemicum in a single test that can be used for screening and investigation of individual cases.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Differentiation of Treponema pallidum Subspecies in Skin Ulceration Clinically Suspected as Yaws in Vanuatu Using Real-Time Multiplex PCR and Serological Methods

Chi¹,

Danavall²,

Taleo³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. We developed a TaqMan-based real-time quadriplex polymerase chain reaction (PCR) to simultaneously detect Treponema pallidum subspecies pallidum, T. pallidum subsp. pertenue, and T. pallidum subsp. endemicum, the causative agents of venereal syphilis, yaws, and bejel, respectively. The PCR assay was applied to samples from skin ulcerations of clinically presumptive yaws cases among children on Tanna Island, Vanuatu. Another real-time triplex PCR was used to screen for the point mutations in the 23S rRNA genes that have previously been associated with azithromycin resistance in T. pallidum subsp. pallidum strains. Seropositivity by the classical syphilis serological tests was 35.5% among children with skin ulcerations clinically suspected with yaws, whereas the presence of T. pallidum subsp. pertenue DNA was only found in lesions from 15.5% of children. No evidence of T. pallidum subsp. pertenue infection, by either PCR or serology was found in~59% of cases indicating alternative causes of yaws-like lesions in this endemic area.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Differentiation of Treponema pallidum Subspecies in Skin Ulceration Clinically Suspected as Yaws in Vanuatu Using Real-Time Multiplex PCR and Serological Methods

Chi¹,

Danavall²,

Taleo³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…The nearly identical 16S rRNA genes of these bovine and human treponemes could be considered a limitation, and future PCR methods might warrant the use of alternative genetic loci. It should be noted that the different Treponema pallidum subspecies that cause very different diseases in humans (e.g., syphilis and yaws) are distinguished thus far only by genes encoding putative surface-exposed proteins (4).…”

Section: Discussionmentioning

confidence: 99%

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

et al. 2009

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This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.

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“…pertenue causes yaws, and T. pallidum subsp. pallidum is the bacteria responsible for both congenital and acquired syphilis (Antal et al, 2002;Centurion-Lara et al, 2006;Mandell et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Treponemal disease in the Old World? Integrated palaeopathological assessment of a 9th–11th century skeleton from north-central Spain

López

Lopez-Garcia

Costilla

et al. 2017

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The hypothesis of an American origin of acquired (venereal) syphilis and other infectious diseases carried by Columbus's crew has been considered for a long time as the most plausible one, and has been supported recently by considerable anthropological and molecular evidence. According to this line of thought, it can be argued that Spain would have been the gateway of acquired syphilis, which quickly expanded after the return of Columbus in the late 15th century. However, the hypothesis that syphilis previously existed in Europe but went unrecognized has also been considered by many authors. The present work reports one Pre-Columbian putative case of venereal syphilis found in a Spanish necropolis and radiocarbon dated to the 9th-11th century. The complexities of carrying out accurate diagnoses in remains from archaeological timeframes are addressed by the combined use of palaeopathological, medical, and histological techniques. The results, which point toward third-stage acquired syphilis, spark discussion about the need to revisit or complement the theories and hypothesis on the origin of syphilis in the light of a growing body of cases from European historical populations.

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Molecular Differentiation ofTreponema pallidumSubspecies

Cited by 74 publications

References 25 publications

Molecular Differentiation of Treponema pallidum Subspecies in Skin Ulceration Clinically Suspected as Yaws in Vanuatu Using Real-Time Multiplex PCR and Serological Methods

Molecular Differentiation of Treponema pallidum Subspecies in Skin Ulceration Clinically Suspected as Yaws in Vanuatu Using Real-Time Multiplex PCR and Serological Methods

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

Treponemal disease in the Old World? Integrated palaeopathological assessment of a 9th–11th century skeleton from north-central Spain

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