Molecular Diagnostics of the Honey Bee Parasites <i>Lotmaria passim</i> and <i>Crithidia</i> spp. (Trypanosomatidae) Using Multiplex PCR

Szalanski, Allen L.; Trammel, Clinton E.; Tripodi, Amber D.; Cleary, Dylan; Rusert, L.; Downey, Danielle

doi:10.1653/024.099.0438

Cited by 14 publications

(8 citation statements)

References 11 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Broadly screening and sequencing positive samples may maximize the probability of detecting potential pathogens, for groups that are likely to contain unexpected or undescribed species, such as Crithidia. A similar approach uses species-specific primers coupled with broad-range primers, allowing for the detection of unexpected species (Stevanovic et al 2016;Szalanski et al 2016;.…”

Section: Protozoansmentioning

confidence: 99%

Endosymbionts that threaten commercially raised and wild bumble bees (Bombus spp.)

Figueroa

Sadd

Tripodi³

et al. 2023

J Poll Ecol

View full text Add to dashboard Cite

Bumble bees (Bombus spp.) are important pollinators for both wild and agriculturally managed plants. We give an overview of what is known about the diverse community of internal potentially deleterious bumble bee symbionts, including viruses, bacteria, protozoans, fungi, and nematodes, as well as methods for their detection, quantification, and control. We also provide information on assessment of risk for select bumble bee symbionts and highlight key knowledge gaps. This information is crucial for ongoing efforts to establish parasite-free programs for future commerce in bumble bees for crop pollination, and to mitigate the problems with pathogen spillover to wild populations.

show abstract

Section: Protozoansmentioning

confidence: 99%

Endosymbionts that threaten commercially raised and wild bumble bees (Bombus spp.)

Figueroa

Sadd

Tripodi³

et al. 2023

J Poll Ecol

View full text Add to dashboard Cite

show abstract

“…Bees were placed on a paper towel for three hours at 22°C to evaporate ethanol preservative from the bees. DNA was extracted using a salting-out protocol with in-house reagents (Sambrook & Russell, 2001) per (Szalanski et al, 2016) and stored at -20°C. Successful DNA extraction was confirmed via PCR amplification of a portion of the honey bee mitochondrial DNA (mtDNA) cytochrome oxidase I and II (COI-COII) region with the use of primers E2 and H2 (Tab.…”

Section: Dna Extractionmentioning

confidence: 99%

“…Samples positive for S. apis yielded a 190 bp amplicon, and samples positive for S. melliferum resulted in a 160 bp amplicon. Trypanosomes Multiplex PCR using primers CBSSU rRNA F2, CBSSU rRNA B4 (Schmid-Hempel & Tognazzo, 2010), and L. passim 18S-F was used to detect trypanosome pathogen species (Szalanski et al, 2016) (Tab. 1).…”

Section: Phorid Flymentioning

confidence: 99%

“…Nocturnal abandonment has been observed in parasitized honey bees, and the ultimate emersion of the fly emerges results in bee death (Core et al, 2012). Taxonomic and morphological identification and detection of understudied internal parasites and pathogens can be tedious and unreliable due to small size and polymorphic variability (Schwarz et al, 2015;Szalanski et al, 2016). Using species-specific PCR primers and other molecular diagnostics, researchers achieve a high degree of success in detecting uncommon parasites and pathogens (Klee et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Diagnostic Survey of Honey Bee, Apis mellifera L., Pathogens and Parasites from Arkansas, USA

Cleary

Szalanski

2022

Journal of Apicultural Science

View full text Add to dashboard Cite

Managed honey bee populations have fluctuated over the past several decades in the U.S. While a single factor has not been identified for these losses, the interaction between multiple biotic and abiotic stressors have been suggested to be responsible. Of major concern are several invasive parasite and pathogen species as well as colony management. A single honey bee colony often suffers from multiple harmful agents, that may act synergistically and cause greater declines in bee health. We conducted a survey to detect known and lesser-known honey bee parasites and pathogens. While previous research has primarily focused on commercially managed colonies, research is limited to pertaining parasite and pathogen prevalence in hobbyist managed colonies. Molecular diagnostics were used to screen 541 Arkansas (AR) honey bee colonies from 107 hobbyist beekeepers for eight A. mellifera parasite and pathogen species. Colony samples were obtained between 2015–2016 and represented forty-seven of the seventy-five AR counties. Vairimorpha ceranae (11.6% occurrence) and parasite Varroa destructor (49.4% occurrence) were relatively common in AR hobbyist colonies. Interestingly, the lesser-studied pathogenic trypanosome species, Lotmaria passim, was detected in 11.3% of the colonies and widespread in twenty of the forty-seven counties sampled. None of the honey bee pathogens Vairimorpha apis, Spiroplasma apis, S. melliferum, Crithidia mellificae, or the parasitic phorid fly, Apocephalus borealis, were detected in the colonies sampled. This study provides an extensive assessment of the parasite and pathogen species occurring at the AR state-level in hobbyist-managed honey bee colonies.

show abstract

“…revealed C. mellificae presence in bee populations with L. passim predominance in A. mellifera worldwide [192]. Building upon this work, a PCR procedure was developed to distinguish between C. mellificae and L. passim in honey bees [193]. A survey of honey bees in different parts of New Zealand revealed high levels of infection with two genotypes of L. passim that remained consistent yearlong with low genetic variation [194].…”

Section: Parasites Pathogens and Pestsmentioning

confidence: 99%

Impact of Biotic and Abiotic Stressors on Managed and Feral Bees

Belsky

Joshi

2019

Insects

View full text Add to dashboard Cite

Large-scale declines in bee abundance and species richness over the last decade have sounded an alarm, given the crucial pollination services that bees provide. Population dips have specifically been noted for both managed and feral bee species. The simultaneous increased cultivation of bee-dependent agricultural crops has given rise to additional concern. As a result, there has been a surge in scientific research investigating the potential stressors impacting bees. A group of environmental and anthropogenic stressors negatively impacting bees has been isolated. Habitat destruction has diminished the availability of bee floral resources and nest habitats, while massive monoculture plantings have limited bee access to a variety of pollens and nectars. The rapid spread and increased resistance buildup of various bee parasites, pathogens, and pests to current control methods are implicated in deteriorating bee health. Similarly, many pesticides that are widely applied on agricultural crops and within beehives are toxic to bees. The global distribution of honey bee colonies (including queens with attendant bees) and bumble bee colonies from crop to crop for pollination events has been linked with increased pathogen stress and increased competition with native bee species for limited resources. Climatic alterations have disrupted synchronous bee emergence with flower blooming and reduced the availability of diverse floral resources, leading to bee physiological adaptations. Interactions amongst multiple stressors have created colossal maladies hitting bees at one time, and in some cases delivering additive impacts. Initiatives including the development of wild flower plantings and assessment of pesticide toxicity to bees have been undertaken in efforts to ameliorate current bee declines. In this review, recent findings regarding the impact of these stressors on bees and strategies for mitigating them are discussed.

show abstract

Molecular Diagnostics of the Honey Bee Parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) Using Multiplex PCR

Cited by 14 publications

References 11 publications

Endosymbionts that threaten commercially raised and wild bumble bees (Bombus spp.)

Endosymbionts that threaten commercially raised and wild bumble bees (Bombus spp.)

Molecular Diagnostic Survey of Honey Bee, Apis mellifera L., Pathogens and Parasites from Arkansas, USA

Impact of Biotic and Abiotic Stressors on Managed and Feral Bees

Contact Info

Product

Resources

About