Molecular Diagnostics in Transfusion Medicine: MALDI-TOF MS for Blood Bankers- The Alternative Approach for Genotyping

Abstract

Although matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry (MALDI-TOF MS) has been previously reported for high throughput blood group genotyping, those reports are limited to only a few blood group systems. This report is timely as promising preliminary results have recently been published from a large cooperative Swiss-German project that aimed to employ MALDI-TOF MS for the molecular detection of the blood groups

Genomic analysis of KEL*03 and KEL*04 alleles among Thai blood donors

“… 11 , 12 , 13 , 14 , 15 Furthermore, matrix-assisted laser desorption/ionisation time of flight mass spectrometry was used to determine blood group alleles in blood donors. 16 , 17 , 18 This method offered high throughput and proved to be less time-consuming than routine serological testing. 16 , 17 , 18 However, genotyping using Luminex-based platforms and matrix-assisted laser desorption/ionisation time of flight mass spectrometry techniques requires costly reagents and equipment, thus making them unsuitable for laboratories with established budgets.…”

“… 16 , 17 , 18 This method offered high throughput and proved to be less time-consuming than routine serological testing. 16 , 17 , 18 However, genotyping using Luminex-based platforms and matrix-assisted laser desorption/ionisation time of flight mass spectrometry techniques requires costly reagents and equipment, thus making them unsuitable for laboratories with established budgets. Since the KEL*03 and KEL*04 allele frequencies in Thai populations remain unknown, this study aimed to detect KEL*03 and KEL*04 alleles among blood donors in central, northern, and southern Thailand using in-house PCR-SSP and to compare the observed allele frequencies with those of other donor populations.…”