Molecular diagnostics and potential therapeutic options for mycobacterium tuberculosis: Where we stand

Khan, Zafran; Ualiyeva, Daniya; Jamal, Khalid; Ali, B.; Ahmad, Fayaz; Amissah, Obed Boadi; Bate, Petuel Ndip Ndip

doi:10.1016/j.meomic.2023.100022

Cited by 4 publications

(3 citation statements)

References 124 publications

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“…Significant level of co-resistance (P<0.001) was also seen between INH and five other drugs (EMB, PZA, STR, LFX and MOX) ( Table 4 ). Co-resistance between INH and other drugs may possibly be due to the high spontaneous mutation rate of INH or due to inhA as such mutations have been previously implicated in co-resistance to INH and PTO as they have the same target, InhA ( 35 , 36 ). The accumulation of drug resistance mutations occurs in a cumulative manner, so the presence of co-resistant strains implies that these strains have been subject to multiple therapies over the lifetime of the strain lineage.…”

Section: Discussionmentioning

confidence: 99%

Distribution of common and rare drug resistance patterns in Mycobacterium tuberculosis clinical isolates revealed by GenoType MTBDRplus and MTBDRsl assay

Khan,

Zhu,

Guan

et al. 2023

J Thorac Dis

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Background Tuberculosis (TB) remains a significant global health emergency caused by Mycobacterium tuberculosis (Mtb). The epidemiology, transmission, genotypes, mutational patterns, and clinical consequences of TB have been extensively studied worldwide, however, there is a lack of information regarding the epidemiology and mutational patterns of Mtb in Pakistan, specifically concerning the prevalence of multi-drug resistant TB (MDR-TB). Methods This study aimed to investigate the incidence of Mtb and associated mutational patterns using the line probe assay (LPA). Previous studies have reported a high frequency of mutations in the rpoB , inhA , and katG genes, which are associated with resistance to rifampicin (RIF) and isoniazid (INH). Therefore, the current study utilized LPA to detect mutations in the rpoB , katG , and inhA genes to identify multi-drug resistant Mtb. Results LPA analysis of a large pool of Mtb isolates, including samples from 241 sputum-positive patients, revealed that 34.85% of isolates were identified as MDR-TB, consistent with reports from various regions worldwide. The most prevalent mutations observed were rpoB S531L and inhA promoter C15T, which were associated with resistance to RIF and INH, respectively. Conclusions This study highlights the effectiveness of GenoType MTBDR plus and MTBDR sl assays as valuable tools for TB management. These assays enable rapid detection of resistance to RIF, INH, and fluoroquinolones (FQs) in Mtb clinical isolates, surpassing the limitations of solid and liquid media-based methods. The findings contribute to our understanding of MDR-TB epidemiology and provide insights into the genetic profiles of Mtb in Pakistan, which are essential for effective TB control strategies.

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Section: Discussionmentioning

confidence: 99%

Distribution of common and rare drug resistance patterns in Mycobacterium tuberculosis clinical isolates revealed by GenoType MTBDRplus and MTBDRsl assay

Khan,

Zhu,

Guan

et al. 2023

J Thorac Dis

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“…In conclusion, the integration of personalized treatment planning into nursing care, through customized therapeutic approaches, the monitoring of molecular biomarkers, and the central role of nursing in personalized medicine, represents a significant advance in healthcare. This approach necessitates a combination of sophisticated genetic analysis, continuous monitoring and adjustment of treatment plans based on quantitative data, and a skilled nursing workforce capable of bridging the gap between molecular biology and patient care [5].…”

Section: The Role Of Nursing In Personalized Carementioning

confidence: 99%

Enhancing personalized medicine through molecular diagnostics: Implications for nursing care

Hong,

Tan

2024

TNS

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The advent of molecular diagnostics, exemplified by technologies such as Polymerase Chain Reaction (PCR) and gene sequencing, represents a pivotal shift towards personalized medicine, fundamentally altering the landscape of healthcare delivery and nursing care. This paper delves into the transformative potential of genetic screening and risk assessment, disease typing and classification, and predictive analysis of treatment responses, highlighting the critical role of molecular diagnostics in facilitating a more nuanced understanding of individual health profiles and tailoring treatment accordingly. The integration of genetic data into treatment planning enables healthcare practitioners to optimize therapeutic strategies, significantly improving patient outcomes. However, this advancement is not without challenges. The ethical considerations surrounding data privacy, the complexity of genetic information, and issues of cost and accessibility are scrutinized, underscoring the need for robust ethical guidelines, continuous professional education, and policy interventions to ensure equitable access to these technologies. Through a comprehensive review, this paper illustrates the intricate interplay between molecular diagnostics and personalized nursing care, advocating for an interdisciplinary approach to harness the full potential of these technological advancements in improving patient care.

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“…Several new antibiotics (bedaquiline, delamanid, and pretomanid) have received approval for the treatment of M. tuberculosis MDR infections [ 37 , 47 ], but unfortunately, resistance has already merged with these new compounds [ 48 , 49 ]. Derivatives of existing anti-tuberculosis medications, such as ethambutol, and repurposed drugs, such as linezolid and clofazimine, have also been proposed but have been threatened by the quick emergence of resistance [ 43 , 49 , 50 , 51 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of New N-acyl Hydrazone Derivatives of Carprofen as Potential Tuberculostatic Agents

Vlad,

Nuță,

Căproiu

et al. 2024

Antibiotics

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N-acyl hydrazone (NAH) is recognized as a promising framework in drug design due to its versatility, straightforward synthesis, and attractive range of biological activities, including antimicrobial, antitumoral, analgesic, and anti-inflammatory properties. In the global context of increasing resistance of pathogenic bacteria to antibiotics, NAHs represent potential solutions for developing improved treatment alternatives. Therefore, this research introduces six novel derivatives of (EZ)-N’-benzylidene-2-(6-chloro-9H-carbazol-2-yl)propanehydrazide, synthesized using a microwave-assisted method. In more detail, we joined two pharmacophore fragments in a single molecule, represented by an NSAID-type carprofen structure and a hydrazone-type structure, obtaining a new series of NSAID-N-acyl hydrazone derivatives that were further characterized spectrally using FT-IR, NMR, and HRMS investigations. Additionally, the substances were assessed for their tuberculostatic activity by examining their impact on four strains of M. tuberculosis, including two susceptible to rifampicin (RIF) and isoniazid (INH), one susceptible to RIF and resistant to INH, and one resistant to both RIF and INH. The results of our research highlight the potential of the prepared compounds in fighting against antibiotic-resistant M. tuberculosis strains.

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Molecular diagnostics and potential therapeutic options for mycobacterium tuberculosis: Where we stand

Cited by 4 publications

References 124 publications

Distribution of common and rare drug resistance patterns in Mycobacterium tuberculosis clinical isolates revealed by GenoType MTBDRplus and MTBDRsl assay

Distribution of common and rare drug resistance patterns in Mycobacterium tuberculosis clinical isolates revealed by GenoType MTBDRplus and MTBDRsl assay

Enhancing personalized medicine through molecular diagnostics: Implications for nursing care

Synthesis and Characterization of New N-acyl Hydrazone Derivatives of Carprofen as Potential Tuberculostatic Agents

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