Molecular Diagnosis of Tuberculosis and Drug Resistance

Lin, Shou-Yean Grace; Desmond, Edward

doi:10.1016/j.cll.2014.02.005

Cited by 25 publications

(30 citation statements)

References 60 publications

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“…If all WT probes and at least one MUT probe are present, interpretation is difficult and a repeat test or confirmation by sequencing is recommended. For this study, however, the assay was not repeated and such results were classified as indeterminate (25).…”

Section: Methodsmentioning

confidence: 99%

MTBDR plus and MTBDR sl Assays: Absence of Wild-Type Probe Hybridization and Implications for Detection of Drug-Resistant Tuberculosis

et al. 2016

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e Accurate identification of drug-resistant Mycobacterium tuberculosis is imperative for effective treatment and subsequent reduction in disease transmission. Line probe assays rapidly detect mutations associated with resistance and wild-type sequences associated with susceptibility. Examination of molecular-level performance is necessary for improved assay result interpretation and for continued diagnostic development. Using data collected from a large, multisite diagnostic study, probe hybridization results from line probe assays, MTBDRplus and MTBDRsl, were compared to those of sequencing, and the diagnostic performance of each individual mutation and wild-type probe was assessed. Line probe assay results classified as resistant due to the absence of wild-type probe hybridization were compared to those of sequencing to determine if novel mutations were inhibiting wild-type probe hybridization. The contribution of absent wild-type probe hybridization to the detection of drug resistance was assessed via comparison to a phenotypic reference standard. In our study, mutation probes demonstrated significantly higher specificities than wild-type probes and wild-type probes demonstrated marginally higher sensitivities than mutation probes, an ideal combination for detecting the presence of resistance conferring mutations while yielding the fewest number of false-positive results. The absence of wild-type probe hybridization without mutation probe hybridization was determined to be primarily the result of failure of mutation probe hybridization and not the result of novel or rare mutations. Compared to phenotypic culture-based drug susceptibility testing, the absence of wild-type probe hybridization without mutation probe hybridization significantly contributed to the detection of phenotypic rifampin and fluoroquinolone resistance with negligible increases in false-positive results. While the number of incident cases of tuberculosis (TB) has been steadily declining over the past decade, the prevalence of drug-resistant disease threatens to reverse these declines (1, 2). The World Health Organization (WHO) estimated that, in 2014, 3.3% of new and 20% of previously treated TB cases were multidrug-resistant (MDR) or were resistant to isoniazid (INH) and rifampin (RIF) (3). Furthermore, 9.7% of these MDR cases were extensively drug resistant (XDR) or were resistant to at least one fluoroquinolone (FQ) in addition to a second-line injectable drug (SLID), amikacin (AMK), kanamycin (KAN), or capreomycin (CAP). Although detection of M/XDR-TB is imperative for effective disease management, less than half of all MDR-TB cases were detected in 2014 (2-4). This detection gap is due, in part, to the lack of low-cost, rapid, and accurate diagnostic technologies for M/XDR-TB (4-6).Conventional culture-based phenotypic drug susceptibility testing (DST) methods can take months to yield results (7). Molecular-based tests, however, can produce results in Ͻ1 day (5,(8)(9)(10)(11)(12). The WHO has endorsed line probe hybridization and mole...

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Section: Methodsmentioning

confidence: 99%

MTBDR plus and MTBDR sl Assays: Absence of Wild-Type Probe Hybridization and Implications for Detection of Drug-Resistant Tuberculosis

et al. 2016

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“…Although childhood TB usually represents less than 5% of disease in industrialized countries, the burden of disease borne by children in less developed, resource-poor countries may be as high as 39% [8,9]. Approximately one-third of the human population can be demonstrated to have immunologic evidence of current or past infection with Mtb [10].…”

Section: Key Pointsmentioning

confidence: 99%

“…Sixty-three percent of TB cases and 88% of MDR-TB cases were foreign-born from countries where TB prevalence and drug-resistance rates are high [10].…”

Section: Key Pointsmentioning

confidence: 99%

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Childhood Tuberculosis

Faddoul

2015

Advances in Pediatrics

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“…Dessa forma, a utilização do teste, antes da cultura, representa agilidade na confirmação do resultado e com isso, o tratamento pode ser iniciado mais precocemente, sem a necessidade de se aguardar a confirmação pela cultura. 6 Tendo em vista a importância de um diagnóstico preciso e precoce para o controle da TB, questiona-se quais as vantagens do teste rápido molecular em comparação à baciloscopia? Em que casos se pode usar esse método?…”

Section: Introductionunclassified

Vantagens e usos do teste rápido molecular para tuberculose: uma revisão integrativa

et al. 2017

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RESUMO: Objetivo: investigar na literatura as vantagens e usos do teste rápido molecular para diagnóstico da tuberculose. Método: revisão integrativa da literatura com condução da questão norteadora elaborada por meio da estratégia PICO (P=Paciente ou Problema, I=Intervenção, C=Comparação ou controle, O=Outcomes ou desfechos). Foi realizada uma busca nas bases de dados BVS, LILACS, MEDLINE e Science Direct com artigos disponíveis na integra, de forma eletrônica publicados entre 2008-2015. Resultados: o teste molecular Gene Xpert, atualmente disponibilizado para o diagnóstico da tuberculose, tem a capacidade de detectar a presença do bacilo causador da doença e identificar se a bactéria tem resistência a rifampicina, em duas horas, além de maior sensibilidade e especificidade em relação à baciloscopia. Conclusões: o teste rápido molecular para o diagnóstico de tuberculose foi bem aceito principalmente pela agilidade, constituindo uma forma mais eficaz de controle da doença, possibilitando início mais rápido do tratamento, sendo importante para minimizar a disseminação da doença. Descritores: Tuberculose; Diagnóstico; Reação em cadeia da polimerase.ABSTRACT: Aim: to investigate though the literature the advantages and uses of the molecular test for fast tuberculosis diagnosis. Method: integrative review of literature with considering the guiding question elaborated by the PICO (P=Patient or Problem, I=Intervention, C=Comparison or control, O=Outcomes) strategy. A research was

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Molecular Diagnosis of Tuberculosis and Drug Resistance

Cited by 25 publications

References 60 publications

MTBDR plus and MTBDR sl Assays: Absence of Wild-Type Probe Hybridization and Implications for Detection of Drug-Resistant Tuberculosis

MTBDR plus and MTBDR sl Assays: Absence of Wild-Type Probe Hybridization and Implications for Detection of Drug-Resistant Tuberculosis

Childhood Tuberculosis

Vantagens e usos do teste rápido molecular para tuberculose: uma revisão integrativa

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