Molecular diagnosis of the central nervous system (CNS) infections

Vilà, Jordi; Bosch, Jordi; Muñoz‐Almagro, Carmen

doi:10.1016/j.eimc.2020.03.001

Cited by 5 publications

(4 citation statements)

References 46 publications

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“…It is worth mentioning that our patient's first CSF analysis had a negative CMV-PCR, and it was not until his fever recurred that the virus was detected on CSF-PCR. A false-negative result in PCR panels has been associated with a low quantity of the pathogen [12].…”

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus (CMV)-Driven Transformation: An Uncommon Cause of a Central Nervous System Pseudotumor

Tejada-Pineda,

Hoyo-Ulloa,

Ortega-Porcayo

et al. 2023

Cureus

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Cytomegalovirus (CMV) is an opportunistic virus that can cause life-threatening neurological diseases in immunocompromised individuals, particularly those with HIV/AIDS. In this case report, a patient presenting with left gait lateralization was found to have a ring-enhancing cerebral mass lesion that was attributed to CMV. To date, only eight similar cases have been documented. When evaluating patients with HIV/AIDS who have cerebral mass lesions, clinicians should keep CMV as a possible cause because prompt antiviral therapy may improve clinical outcomes.

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Section: Discussionmentioning

confidence: 99%

Cytomegalovirus (CMV)-Driven Transformation: An Uncommon Cause of a Central Nervous System Pseudotumor

Tejada-Pineda,

Hoyo-Ulloa,

Ortega-Porcayo

et al. 2023

Cureus

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“…The microbiological diagnosis of IMD has changed radically in recent years with the introduction of the so-called molecular techniques based on the amplification of nucleic acids, thus gaining greater sensitivity [ 18 ] ( Table 2 ). Previously, this diagnosis was based exclusively on direct sample staining, essentially Gram staining, and microbiological culture of cerebrospinal fluid, blood cultures or some tissues with a highly variable sensitivity depending on the microorganism and bacterial load (10-95%).…”

Section: What Is the Role Of New Tools In The Diagnosis Of Invasive M...mentioning

confidence: 99%

Meningococcal meningitis in Spain in the Horizon 2030: A position paper

Moraga-Llop,

Andradas,

Blesa-Baviera

et al. 2024

Rev Esp Quimioter

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Meningococcal meningitis (MM) and invasive meningococcal disease remain a major public health problem that generates enormous public alarm. It is caused by Neisseria meningitidis, a Gram-negative diplococcus with an enormous capacity for acute and rapidly progressive disease, both episodic and epidemic in nature, with early diagnosis and treatment playing a major role. It occurs at any age, but is most common in children under 5 years of age followed by adolescents. Although most cases occur in healthy people, the incidence is higher in certain risk groups. Despite advances in reducing the incidence, it is estimated that in 2017 there were around 5 million new cases of MM worldwide, causing approximately 290,000 deaths and a cumulative loss of about 20,000,000 years of healthy life. In Spain, in the 2021/22 season, 108 microbiologically confirmed cases of MM were reported, corresponding to an incidence rate of 0.23 cases per 100,000 inhabitants. This is a curable and, above all, vaccine-preventable disease, for which the World Health Organisation has drawn up a roadmap with the aim of reducing mortality and sequelae by 2030. For all these reasons, the Illustrious Official College of Physicians of Madrid (ICOMEM) and the Medical Associations of 8 other provinces of Spain, have prepared this opinion document on the situation of MM in Spain and the resources and preparation for the fight against it in our country. The COVID-19 and Emerging Pathogens Committee of ICOMEM has invited experts in the field to participate in the elaboration of this document.

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“…Therefore, to optimize directed therapy and hopefully improve patient outcomes, the early and accurate identification of the etiological agent is critical [3][4][5]. This has been made possible through the development of multiplex PCR (M-PCR) tests to detect the most common microorganisms causing encephalitis, meningitis, or meningoencephalitis (ME) in cerebrospinal fluid (CSF) [6]. At present, there are two M-PCR tests on the market: the Filmarray ME Panel, BioFire Diagnostics (Salt Lake City, UT, USA), launched in 2015, Diagnostics 2024, 14, 802 2 of 12 which is based on a nested PCR followed by a melt curve analysis in a microarray format, and the recently introduced QIAstat-Dx ME panel cassette (QIAGEN, Hilden, Germany), which is based on a multiplex real-time PCR platform.…”

Section: Introductionmentioning

confidence: 99%

An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis

Cuesta,

Puerta-Alcalde,

Vergara

et al. 2024

Diagnostics

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The rapid and broad microbiological diagnosis of meningoencephalitis (ME) has been possible thanks to the development of multiplex PCR tests applied to cerebrospinal fluid (CSF). We aimed to assess a new multiplex PCR panel (the QIAstat-Dx ME panel), which we compared to conventional diagnostic tools and the Biofire FilmArray ME Panel. The pathogens analyzed using both methods were Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae, Enterovirus, herpes simplex virus 1–2, human herpesvirus 6, human parechovirus, varicella zoster virus, and Cryptococcus neoformans/gattii. We used sensitivity, specificity, PPV, NPV, and kappa correlation index parameters to achieve our objective. Fifty CSF samples from patients with suspected ME were included. When conventional methods were used, 28 CSF samples (56%) were positive. The sensitivity and specificity for QIAstat-Dx/ME were 96.43% (CI95%, 79.8–99.8) and 95.24% (75.2–99.7), respectively, whereas the PPV and NPV were 96.43% (79.8–99.8) and 95.24% (75.1–99.7), respectively. The kappa value was 91.67%. Conclusions: A high correlation of the QIAstat-Dx ME panel with reference methods was shown. QIAstat-Dx ME is a rapid-PCR technique to be applied in patients with suspected ME with a high accuracy.

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Molecular diagnosis of the central nervous system (CNS) infections

Cited by 5 publications

References 46 publications

Cytomegalovirus (CMV)-Driven Transformation: An Uncommon Cause of a Central Nervous System Pseudotumor

Cytomegalovirus (CMV)-Driven Transformation: An Uncommon Cause of a Central Nervous System Pseudotumor

Meningococcal meningitis in Spain in the Horizon 2030: A position paper

An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis

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