Molecular diagnosis of infectious diseases in dermatology

Karan, Kalpita R; Torres, Gisela; Rády, Peter L.; Hughes, Thomas K.; Payne, Deborah; Tyring, Stephen K.

doi:10.1016/j.jaad.2004.08.052

Cited by 29 publications

(8 citation statements)

References 92 publications

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“…In recent years, comprehensive analysis of bacteria using molecular methods without cultivation has been applied to areas of wound management (12,13). However, the diagnostic value appears to be relatively low or varies with the different clinical types of bacteria (14). Most importantly, only identifying the presence of specific bacteria by molecular methods does not mean diagnosis of infection in the field of wound management.…”

Section: Introductionmentioning

confidence: 99%

Novel biomarkers for the detection of wound infection by wound fluid RT‐PCR in rats

Asada¹,

Nakagami²,

Minematsu³

et al. 2011

Experimental Dermatology

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Wound infection is a form of host damage resulting from an imbalance in pathogen virulence and the host immune response. However, at present, diagnosis is based solely on bacterial numbers or inflammatory signs and is therefore not precise. Thus, infection diagnosis requires indicators of both of these factors. We focused on wound fluid because it includes both bacteria and host cells. The purpose of this study was to establish biomarkers that reflect both bacterial and host factors using the reverse transcription-polymerase chain reaction method on the centrifugal precipitation of wound fluids (wound fluid RT-PCR). We created full thickness wounds in animal models of the three groups: control, colonization and infection, which were conditioned by administration of different concentrations of Pseudomonas aeruginosa dispersion. Messenger RNA expression in bacteria and host cells was analysed. Expression of bacterial housekeeping genes was detected in the samples in the colonization and infection groups. Expression of host housekeeping genes was detected in all samples from the three groups. Expression of toxA, encoding the virulence factor exotoxin A, was detected in 90% of samples in the infection group only. Expression of Foxp3, encoding the transcription factor forkhead box P3, was detected in 100% of samples only in the colonization group. These results revealed that wound fluid RT-PCR analysis reflected both bacterial virulence and the host immune status, and we determined the combination of novel biomarkers that can discriminate these three groups. We anticipate that wound fluid RT-PCR could be applied in the future to diagnose wound infection.

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Section: Introductionmentioning

confidence: 99%

Novel biomarkers for the detection of wound infection by wound fluid RT‐PCR in rats

Asada¹,

Nakagami²,

Minematsu³

et al. 2011

Experimental Dermatology

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“…Other diagnostic methods include polymerase chain reaction and serologic tests, which are not readily available [2][3][4][5][6][7][8].…”

Section: Discussionmentioning

confidence: 99%

Painful ulcers in a 5-year-old girl

et al. 2011

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“…Tab. 4, [39,40]). Die Verfahren sind mittlerweile so standardisiert und reproduzierbar, dass die PCR-Analyse unter anderem für Mycobacterium tuberculosis, Herpes simplex 1 und 2, HIV und Hepatitis C von öffentlichen Kontrollgremien wie der FDA als Diagnostikmethode zugelassen worden ist.…”

Section: Moderne Molekulare Methodenunclassified

Molekulargenetik in der dermatologischen Diagnostik

Braun‐Falco

Ruzicka

2012

Hautarzt

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Modern molecular techniques have tremendously expanded our knowledge about the biologic processes in healthy individuals as well as our understanding about the pathologic events in an increasing number of dermatological diseases. These technologies initially came from basic molecular biology and genetic research but have become firmly anchored in clinical diagnosis approaches. Included in this group are immunohistochemistry (IHC), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), chromogen in situ hybridization (CISH), comparative genomic hybridization (CGH), and microarray technology. IHC and PCR already belong to the armamentarium for routine daily diagnostics due to their high degree of standardization and reproducibility, ease of use and relatively low costs. Others like FISH and CISH are currently employed for specific indications in a growing number of larger laboratories, whereas CGH and microarray technology still remain in the hands of a few highly specialized laboratories. These new ancillary methods will help to improve diagnostic accuracy particularly in cases in which conventional histopathology is ambiguous. In addition, they will provide new and important information concerning the prognosis, progression and response rate to therapies in several particular malignant diseases.

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Molecular diagnosis of infectious diseases in dermatology

Cited by 29 publications

References 92 publications

Novel biomarkers for the detection of wound infection by wound fluid RT‐PCR in rats

Novel biomarkers for the detection of wound infection by wound fluid RT‐PCR in rats

Painful ulcers in a 5-year-old girl

Molekulargenetik in der dermatologischen Diagnostik

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