Molecular diagnosis of
            <i>Leishmania</i>
            spp. in dogs of a subtropical locality of Argentina

Ascencio, Mariano E.; Sarmiento, Néstor Fabián; Schnittger, Leonhard; Florín-Christensen, Mónica; Rodríguez, Anabel Elisa

doi:10.1111/tbed.13313

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…This leads to DNA with enough integrity to remain as a template for the amplification of target sequences for diagnosis. The use of the resin is attractive because it is a simple, fast, and economic method especially in lowresource settings (Turan et al 2015;Ascencio et al 2019).…”

Section: Discussionmentioning

confidence: 99%

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Use of Chelex-100 for the molecular diagnosis of five animal pathogens

Tomazic¹,

Hamer²,

Bustos³

et al. 2021

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The goal of this work was to evaluate the nutritional quality in 3 cultivars of alfalfa, belonging to different winter dormancy (GRI), subjected to two cutting frequencies during one year of cultivation, based on the abundant information existing in studies carried out in productivity, coverage and persistence, not so in the determination of quality of forage subjected to different frequencies of cuts and contrasting GRI. Three cultivars (GR6-Verzy), (GR9-Mecha) and (GR10-Ruano) were tested. The experimental design was a complete split plot with four repetitions. Two treatments defined by the cutting intervals were established: T1: 25 days and T2: 35 days. These were specified for spring, summer and fall seasons. For winter the interval was 45 and 55 days in T1 and T2, respectively. The evaluated variables were: percentage of dry matter (% DM), percentage of crude protein (% PB), percentage of neutral detergent fiber (% FDN) and percentage of acid detergent fiber (% FDA). Significant differences were found for all the nutritional variables studied, for both treatment with p <0.05 with InfoStat program. Interaction between cultivars and treatment was observed for % PB. The 35 days cutoff frequency of yielded the lowest percentages of PB and the highest percentages of FDN and FDA. Hence the forage resulting from this frequency is of a lower quality, leading to decreased digestibility and lower performance of pasture for livestock.

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Section: Discussionmentioning

confidence: 99%

“…parasites per milliliter for this PCR. Additionally, a recent study performed by Ascencio et al (2019) has shown that Chelex-100 resin was applied to extract DNA from whole blood, being useful for the diagnosis of canine leishmaniasis.…”

Section: Discussionmentioning

confidence: 99%

Use of Chelex-100 for the molecular diagnosis of five animal pathogens

Tomazic¹,

Hamer²,

Bustos³

et al. 2021

FAVE Cs Vet

Self Cite

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“…[22][23][24] Likewise, two cases of L. infantum-caused CL have been described in Paraguay, near the outbreak area discussed here. 25,26 We can speculate on different factors that would influence the appearance of CL because of L. infantum in the urban and periurban areas under study, including a high density of the vector, deficiencies in basic sanitary services (waste management and sanitation), deforestation, activities undermining vector control (inappropriate waste recycling and artisanal brickworks, among others), soil characteristics, the high concentration of families living in poverty and facing difficulties in accessing protection measures, and the presence of dogs infected by L. infantum in the area of study [27][28][29] All of this multicausality could explain the occurrence of the first outbreak of this magnitude in a very dense urban and periurban area.…”

Section: Discussionmentioning

confidence: 99%

“…3,7,8 Our analysis suggests an urbanization of CL, which is consistent with similar clinical and epidemiological patterns observed in the Mediterranean basin, 30,31 and the presence of good transmission vectors and high prevalence of canine infection, which facilitate the spread of the disease. 28,29 These circumstances could herald a much greater impact and magnitude of CL in the region in the near-to-medium term.…”

Section: Discussionmentioning

confidence: 99%

Autochthonous Outbreak of Cutaneous Leishmaniasis due to Leishmania infantum in Corrientes Province, Argentina

Soto

Encinas

Deschutter

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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Endemic cutaneous leishmaniasis (CL) in northern Argentina has traditionally been caused by Leishmania braziliensis. This study aims to describe an outbreak of Leishmania infantum-caused human CL in the Department Capital of Corrientes Province, Argentina. We retrospectively analyzed the reported cases of CL in this area from May 2015 to December 2016. Eighty cases of CL were clinically and analytically diagnosed, and there was one case of visceral leishmaniasis in a boy who also had CL. Patients' median age was 33.6 years (range 1-89 years), and 18.5% were younger than 15 years; the male:female ratio was 3.5:1. Cases lived mostly in the municipality of Corrientes (72.8%), whereas 27.2% resided in Riachuelo. Although 67.9% had a single lesion, 32.1% had several. Molecular analyses showed that L. infantum was the causative species in all cases. Our results show that for the first time, there was an outbreak of CL by L. infantum in an urban area of Argentina.

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Exploring the paradox of defense between host and Leishmania parasite

Kumari

Singh

2022

International Immunopharmacology

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Molecular diagnosis of Leishmania spp. in dogs of a subtropical locality of Argentina

Cited by 4 publications

References 28 publications

Use of Chelex-100 for the molecular diagnosis of five animal pathogens

Use of Chelex-100 for the molecular diagnosis of five animal pathogens

Autochthonous Outbreak of Cutaneous Leishmaniasis due to Leishmania infantum in Corrientes Province, Argentina

Exploring the paradox of defense between host and Leishmania parasite

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