Molecular Diagnosis in Resource-Limited Settings

Rodrigues, Camilla; Desai, Niyati; Fernandes, Helen

doi:10.1016/j.clinmicnews.2016.03.004

Cited by 7 publications

(4 citation statements)

References 49 publications

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“…The presence of C580 Y in P. falciparum populations in Ranong and in Southern Thai provinces near the Myanmar border is expected given previous reports on the spread of resistance to the front-line therapy drug artemisinin in parasites in Thailand over the past 5 years [50]. Clinical investigation by the Tracking Resistance to Artemisinins Collaboration (TRAC) and molecular investigation conducted by the MalariaGEN Plasmodium falciparum project do not extend to Southern regions on the Malaysian border, and to the authors’ knowledge the artemisinin resistance genotypes observed throughout the greater Mekong subregion have not been reported in Yala [19, 51].…”

Section: Discussionmentioning

confidence: 98%

Molecular detection of drug resistant malaria in Southern Thailand

Noisang

Prosser

Meyer

et al. 2019

Malar J

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Background Drug resistance within the major malaria parasites Plasmodium vivax and Plasmodium falciparum threatens malaria control and elimination in Southeast Asia. Plasmodium vivax first-line treatment drug is chloroquine together with primaquine, and the first-line treatment for P. falciparum malaria is artemisinin in combination with a partner drug. Plasmodium vivax and P. falciparum parasites resistant to their respective first-line therapies are now found within Southeast Asia. The resistance perimeters may include high transmission regions of Southern Thailand which are underrepresented in surveillance efforts. Methods This study investigated blood samples from malaria centres in Southern Thailand. Genetic loci associated with drug resistance were amplified and sequenced. Drug resistance associated genes Pvmdr1 , Pvcrt - o , Pvdhfr , and Pvdhps were characterized for 145 cases of P. vivax malaria, as well as the artemisinin resistance-associated Pfkelch13 gene from 91 cases of P. falciparum malaria. Results Plasmodium vivax samples from Southern Thai provinces showed numerous chloroquine and antifolate resistance-associated mutations, including SNP and Pvcrt - o K10 -insertion combinations suggestive of chloroquine resistant P. vivax phenotypes. A high proportion of the C580 Y coding mutation (conferring artemisinin resistance) was detected in P. falciparum samples originating from Ranong and Yala (where the mutation was previously unreported). Conclusions The results demonstrate a risk of chloroquine and antifolate resistant P. vivax phenotypes in Southern Thailand, and artemisinin resistant P. falciparum observed as far south as the Thai–Malaysian border region. Ongoing surveillance of antimalarial drug resistance markers is called for in Southern Thailand to inform case management. Electronic supplementary material The online version of this article (10.1186/s12936-019-2903-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 98%

Molecular detection of drug resistant malaria in Southern Thailand

Noisang

Prosser

Meyer

et al. 2019

Malar J

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“…They employ the principles of molecular markers or culture-based detection systems [21]. These devices follow the ASSURED criteria, which means that they should be affordable, sensitive, specific, user-friendly, rapid, robust, equipment-free, and delivered to the needy [22] [23]. In addition to being inexpensive for mass production, it is also useful on-site for low-skilled personnel [24].…”

Section: Introductionmentioning

confidence: 99%

Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples

2024

IJFMR

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Pathogens emerge and spread from a variety of sources including food, water, air, and clinical samples, resulting in disease epidemics in the community. The early detection of pathogens in food and water samples can help prevent illness transmission. Therefore, detection of harmful bacteria is vital in this setting. Food and water-borne illnesses are more common in underdeveloped and emerging countries than in affluent countries. Because of the lack of well-equipped centralized laboratory facilities, many cases are unreported, resulting in an underestimation of the bioburden of the disease. To address the limitations of culture-based approaches, infections are discovered using advanced methods such as nucleic acid or immunological methods, which are costly and require extensive sample preparation. This study focused on the development of a paper-based Portable Culture Device (PCD) capable of detecting Listeria monocytogenes. The device, optimized at a substrate concentration of 3 gL-1 and a temperature range of 28°C-37°C, can detect cell counts of up to 10^6 CFU ml^-1. The development of paper-based microfluidic detection devices has the potential to overcome the limitations of current organism-detection technologies. These devices adhere to ASSURED standards, which require them to be inexpensive, sensitive, user-friendly, quick, resilient, equipment-free, and supplied to the needy. This could help solve many of the issues associated with centralized testing facilities and simplify on-site microbe detection.

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“…On the other hand, they also present certain disadvantages. Most molecular techniques require expensive equipment and trained personnel [13] . Moreover, the DNA isolation and extraction is still very costly, making the DNA-based methods inappropriate for fields of mass production.…”

Section: Introductionmentioning

confidence: 99%

A comparison of different DNA extraction methods and molecular techniques for the detection and identification of foodborne pathogens

2021

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<abstract> <p>Foodborne infections continue to plague Europe. Food safety monitoring is in crisis as the existing techniques for detecting pathogens do not keep up with the global rising of food production and consumption. Thus, the development of innovative techniques for detecting and identifying pathogenic bacteria has become critical. The aim of the present study was firstly to develop an innovative simple and low cost method of extracting bacterial DNA from contaminated food and water samples with <italic>Salmonella enteric</italic> subsp. <italic>enteric</italic> serovar Typhimurium and <italic>Listeria monocytogenes</italic> and its comparison with two commercial DNA extraction kits (Qiagen, Macherey-Nagel). Finally, pathogens' detection using two molecular techniques (PCR-electrophoresis, LAMP), in order to evaluate the best combination of DNA extraction and identification based on their sensitivity, cost, rapidity and simplicity. Considering the above criteria, among them, best was proved an in-house bacterial DNA extraction method, based on the chloroform-isoamyl alcohol protocol, with certain modifications. This technique showed statistically similar results in terms of sensitivity, compared to the commercial kits, while at the same time maintained high rapidity and much lower cost. Lastly, between the molecular techniques, LAMP was found more promising considering its simplicity, high rapidity and sensitivity. Conclusively, the in-house DNA extraction method along with the LAMP technique, was proven to be the best among the presented combinations.</p> </abstract>

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Molecular Diagnosis in Resource-Limited Settings

Cited by 7 publications

References 49 publications

Molecular detection of drug resistant malaria in Southern Thailand

Molecular detection of drug resistant malaria in Southern Thailand

Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples

A comparison of different DNA extraction methods and molecular techniques for the detection and identification of foodborne pathogens

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