Molecular determinants of the A11 and A12 allotypic specificities in rabbit immunoglobulin

Prahl, James W.; Mandy, William J.; Todd, Charles W.

doi:10.1021/bi00840a042

Cited by 80 publications

(23 citation statements)

References 24 publications

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“…Prahl et al found a replacement of methionine for threonine in the hinge region of rabbit y chains that correlated with the All and Al2 allotypes (9). In addition, we have reported that there are three amino-acid positions where aminoacid interchanges occur in the carboxyl-terminal heptapeptides from light chains of b4, b5, and b6 allotypes (10).…”

supporting

confidence: 52%

Structural Basis of the A14 and A15 Allotypic Specificities in Rabbit Immunoglobulin G

Appella

Chersi

Mage

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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The structural basis for the A14 and A15 allotypic specificities of rabbit immunoglobulin G was investigated with Fc fragments prepared from homozygous rabbits. The presence of threonine in the C-y2 region (position 309: Eu numbering) correlates with the A14 allotype; the presence of alanine at this same position correlates with the A15. Now that the sequence of the Cy2 region of rabbit IgG has been completely elucidated, a high degree of homology of the constant region domains of rabbit and human -y chains is apparent (about 60-80%).Allotypic specificities of rabbit immunoglobulin G are associated with at least three genetic loci, a, b, and c. The allotypic specificities al, a2, and a3 are present on the Fd portion of the heavy chains and appear to be controlled by Mendelian alleles at the locus designated a(l). Wilkinson has reported structural differences in amino-terminal peptides of both Sy and a chains that correlate with a locus allotypes (2, 3). These findings, as well as various other experimental observations, have led to the view that the a markers are associated with the variable region of rabbit heavy chains (VH). Whereas the a markers are found in association with various immunoglobulin classes (4), allotypic determinants such as A8 and A10 (5), All and A12 (6), and A14 and A15 (7) are unique to the oy heavy chains of rabbits. The A14 and A15 markers have been localized in the Fc portion of IgG (7). Genetic control of these C-y markers is linked to the a locus, but occasional recombination may occur (8).Studies on the amino-acid sequence of the heavy and light chains have related some rabbit allotypes to sequence variations. Prahl et al. found a replacement of methionine for threonine in the hinge region of rabbit y chains that correlated with the All and Al2 allotypes (9). In addition, we have reported that there are three amino-acid positions where aminoacid interchanges occur in the carboxyl-terminal heptapeptides from light chains of b4, b5, and b6 allotypes (10).In the present study, the structural variation present in Sy chains of IgG from rabbits homozygous for A14 and A15 allotypes has been investigated. It will be shown that the presence of threonine at position 309 (Eu numbering) in the sequence correlates with the A14 allotype, and the presence of alanine at this same position correlates with the A15 allotype. MATERIALS AND METHODSRabbits used in this study were of known parentage from closed colonies bred at the National Institutes of Health. Their sera were typed for a markers by an Ouchterlony gel-1341 diffusion method (11), and for A14 and A15 by hemagglutination inhibition (12). We used pooled sera of four AaAaa'A 4-A14 rabbits and of ten Aa'AalA l5A 5 rabbits. IgG was prepared from rabbit sera by the rivanol method (13), followed by ammonium sulfate precipitation (35% of saturation) and chromatography on DEAE-cellulose with 17.5 mM sodium phosphate, pH 7.2. The Fc fragment was prepared from IgG of each of the allotypically homozygous rabbits by papain digestion (14). The fr...

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supporting

confidence: 52%

Structural Basis of the A14 and A15 Allotypic Specificities in Rabbit Immunoglobulin G

Appella

Chersi

Mage

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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“…In general, allotypic specificities present on heavy chains of individual IgG molecules in rabbits heterozygous at the VHa locus and also at the C7 locus [which controls allotypic specificities dll and d12, and e14 and e15 on the constant portion of the y chain (16,17)] are controlled by either the maternal or the paternal chromosome; i.e., the VH and CH regions are controlled by the VHa and C, genes, which are cis to each other (17)(18)(19). Recently, however, Landucci-Tosi and Tosi (20), using IgG preparations fractionated on the basis of allotypic characteristics, have reported finding alel5 and a2el4 recombinant IgG molecules from ale'4/a2e15 rabbits.…”

mentioning

confidence: 99%

Recombinant Rabbit Secretory Immunoglobulin Molecules: Alpha Chains with Maternal (Paternal) Variable-Region Allotypes and Paternal (Maternal) Constant-Region Allotypes

Knight

Malek

Hanly

1974

Proc. Natl. Acad. Sci. U.S.A.

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“…Studies of IgG proteins have shown that the dll/d12 allotypic specificities correlate with a methionine/threonine interchange at residue 224 (28) and that the e14/e15 allotypic specificities correlate with a threonine/alanine interchange at residue 309 (29). The nucleotide sequence of p2a2 encodes methionine at position 224 (dll) and alanine at position 309 (el5) (boxed residues in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Double-stranded cDNA was passed over a BioGel A-150m column (Bio-Rad) to select the largest molecules (average size, 600 nucleotides). Terminal deoxynucleotidyltransferase (P-L Biochemicals) was then used to add [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] dCMP molecules to each 3' end (15). The dCMP-tailed cDNA was hybridized to Pst I-digested, dGMP-tailed pBR322 as described (16).…”

mentioning

confidence: 99%

Heavy chain genes of rabbit IgG: isolation of a cDNA encoding gamma heavy chain and identification of two genomic C gamma genes.

Martens¹,

Moore²,

Steinmetz³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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A cDNA library was constructed by using rabbit spleen poly(A)+RNA as template, and from this library was isolated a cDNA clone, p2a2, that encodes 179 amino acids of the heavy chain of rabbit IgG. The nucleotide sequence of p2a2 showed that it encodes the COOH-terminal eight amino acids of the CHI domain, the hinge region, the CH2 domain, and the NH2-terminal half of the CH3 domain of Cr, Southern blot hybridization analysis of rabbit sperm DNA showed that two EcoRI fragments hybridized strongly with the Cy, cDNA. The p2a2 cDNA was used as a probe to isolate recombinant Charon 4A phage clones containing C,, sequences from a genomic library of rabbit liver DNA. Two distinct DNA segments were identified by restriction mapping and hybridization analysis, suggesting that the haploid rabbit genome may contain two different C,, genes.

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Molecular determinants of the A11 and A12 allotypic specificities in rabbit immunoglobulin

Cited by 80 publications

References 24 publications

Structural Basis of the A14 and A15 Allotypic Specificities in Rabbit Immunoglobulin G

Structural Basis of the A14 and A15 Allotypic Specificities in Rabbit Immunoglobulin G

Recombinant Rabbit Secretory Immunoglobulin Molecules: Alpha Chains with Maternal (Paternal) Variable-Region Allotypes and Paternal (Maternal) Constant-Region Allotypes

Heavy chain genes of rabbit IgG: isolation of a cDNA encoding gamma heavy chain and identification of two genomic C gamma genes.

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