Molecular determinants of protein reabsorption in the amphibian kidneys

Seliverstova, Elena V.; Романова, И. В.; Np, Prutskova

doi:10.1016/j.acthis.2021.151760

Cited by 3 publications

(1 citation statement)

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“…Они представляют собой потенциалзависимые хлоридные каналы плазматической мембраны и внутриклеточные Cl -/H + -обменники, вовлеченные, в частности, в трансэпителиальный транспорт и эндоцитоз [12]. В проксимальном канальце в отличие от других частей нефрона одновременно с реабсорбцией воды и ионов происходит всасывание низкомолекулярных белков с помощью рецепторов эндоцитоза мегалина и кубилина, что показано у млекопитающих [13], рыб [14] и амфибий [15][16][17]. Внутриклеточные хлоридные каналы, ClC-3 и ClC-5 участвуют в установлении рН различных внутриклеточных компартментов в процессе эндоцитоза, от их вовлечения в ацидофикацию эндоцитозных везикул зависит эффективность гидролиза белка в лизосомах [4,12,18].…”

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Effect of changes in water-salt balance on ion- and osmoregulatory renal functions in the lake frog

Prutskova,

Seliverstova,

Kutina

2023

Lab Anim Sci Rus

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Changes in the water-salt balance and excretion of ions by the kidney after subcutaneous injections of hypertonic and isotonic NaCl solutions and dehydration were analyzed in lake frogs (Pelophylax ridibundus). The introduction of 0,75 M NaCl and dehydration for 1 hour increased the serum osmolality and Na+ and Cl– concentrations, and injections of isotonic solution did not affect these parameters. The applied treatments increased the level of Na+ and Cl– in the urine, and renal excretion of these ions. The most pronounced changes, including an increase in diuresis and glomerular filtration rate, were found after 0,75 М NaCl injection. In renal proximal tubules, changes in the pattern of the chloride channel (ClC-5), presumably involved in the process of protein endocytosis, were detected. An increase in the number of ClC-5-immunopositive tubule profiles and the intensity of the fluorescent signal in the apical cytoplasm of epithelial cells were revealed by immunohistochemistry and confocal microscopy. Studied effects of hypernatremia, hypervolemia and dehydration may be useful for further investigations of kidney function and mechanisms of epithelial tubular transport in frog models.

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