Molecular determinant of sensing extracellular pH in classical transient receptor potential channel 5

Involvement of TRP channels in the CO2 chemosensitivity of locus coeruleus neurons

“…The high level of expression of TRPC5 mRNAs in the LC is interesting because TRPC5 and TRPC4 have been previously reported to be activated and inhibited by acidic pH (Kim et al 2008;Semtner et al 2007). Therefore, whether the pH sensitivity allows them to act in neuronal CO 2 chemosensitivity is uncertain.…”

“…Second, although several TRP channels are pH-sensitive, most of them are inhibited by acidic pH (Clapham 2003). TRPC4 and TRPC5 are the only known TRP channels that are activated at pH 7.5-6.5 and inhibited when pH is Ͻ6.0 (Kim et al 2008;Semtner et al 2007). We have found evidence for the apparent differences in their potential contribution to the hypercapnic responses of LC neurons.…”

Identification of a Membrane-targeting Domain of the Transient Receptor Potential Canonical (TRPC)4 Channel Unrelated to Its Formation of a Tetrameric Structure

“…The whole cell configuration was used to measure TRPC channel current in HEK cells as described previously (13)(14)(15)(16)(17). Cells were left for 10 -15 min to attach to coverslips.…”

TRPC5 Channel Is the Mediator of Neurotrophin-3 in Regulating Dendritic Growth via CaMKII in Rat Hippocampal Neurons

“…Additionally, carbachol (Andrade, 1991) or histamine (Schaefer et al, 2000), agonists for G q/11 protein-coupled muscarinic or histamine 1 receptors, respectively, were used to activate overexpressed TRPC5 and other TRPCs (Vazquez et al, 2003;Kwon et al, 2007) in cell lines. Meanwhile, reducing extracellular pH (Semtner et al, 2007;Kim et al, 2008) or elevating [Ca 2ϩ ] i (Blair et al, 2009) were reported to potentiate the activation of TRPC5 in HEK293 cells. Our results here suggest that NT-3 is a physiological activator for TRPC5 in the CNS.…”