Molecular detection of three intestinal cestode species (Raillietina echinobothrida, R. tetragona, R. cesticillus) from poultry in Thailand

<i>Raillietina cesticillus</i> infection causes reduced egg production in chickens in a windowless poultry house

“…Many eggs were observed in the gravid proglottids, and each egg contained one oncosphere. DNA was extracted from the tapeworms using a Nucleospin Tissue Kit (Macherey-Nagel, Düren, Germany), and PCR was performed following previously reported protocols [7]. Tapeworms were identified as R. cesticillus by morphological appearance [2,4,5,9,11] and PCR results [7].…”

“…DNA was extracted from the tapeworms using a Nucleospin Tissue Kit (Macherey-Nagel, Düren, Germany), and PCR was performed following previously reported protocols [7]. Tapeworms were identified as R. cesticillus by morphological appearance [2,4,5,9,11] and PCR results [7]. The PCR product was subjected to sequencing and analyzed using the BLASTn algorithm, which confirmed a 100% similarity to the sequence in the GenBank database bearing accession number MW291976.1, thereby identifying R. cesticillus.…”