Molecular detection of Theileria, Babesia, and Hepatozoon spp. in ixodid ticks from Palestine

Azmi, Kifaya; Ereqat, Suheir; Nasereddin, Abedelmajeed; Al-Jawabreh, Amer; Baneth, Gad; Abdeen, Ziad

doi:10.1016/j.ttbdis.2016.03.003

Cited by 29 publications

(24 citation statements)

References 35 publications

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“…were detected in 31 animals out of 45 (68.9%) by ME, which was similar to the rate reported by Gadelrb [19] in Khartoum State, Central Sudan (68%) but higher than that found by Osman et al [20], South-Darfur State, western, Sudan (4.9%). Different prevalence rates were recorded in different parts of the world; 29% in Iran [21], 4.8% in Tunisia [22] and 1.2% in Maghreb [23]. The high prevalence rate determined in the current study compared to other regions may be attributed to differences in climatic conditions and sample collection times as observed by Yaghfoori [24].…”

Section: Discussionmentioning

confidence: 49%

Prevalence, First Molecular Identification and Characterization of Theileria lestoquardi in Sheep in Alhuda National Sheep Research Station, Al Gezira State, Sudan

Ahmed

Salih

et al. 2019

AJOB

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Section: Discussionmentioning

confidence: 49%

Prevalence, First Molecular Identification and Characterization of Theileria lestoquardi in Sheep in Alhuda National Sheep Research Station, Al Gezira State, Sudan

Ahmed

Salih

et al. 2019

AJOB

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“…in hard ticks in Israel and the adjacent Palestinian Authority have not reported the presence of DNA compatible with B. negevi n. sp. strengthen the idea that O. tholozani could be its vector [9,10].…”

Section: Discussionmentioning

confidence: 66%

“…Four of the dogs were co-infected with Borrelia persica (Dschunkowsky, 1913) Steinhaus, 1946, a relapsing fever agent which causes disease in humans, dogs and cats, and is transmitted by the argasid tick Ornithodoros tholozani Laboulbène & Mégnin [6][7][8]. This led to the hypothesis that this previously unknown Babesia sp., which was not identified in earlier molecular studies of pathogens transmitted by hard ticks in Israel and its vicinity [9,10], might be transmitted by O. tholozani. We therefore surveyed soft ticks in Israel for the presence of piroplasms.…”

Section: Introductionmentioning

confidence: 64%

A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp.

et al. 2020

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Introduction: Babesiosis is a protozoan tick-borne infection associated with anemia and life-threatening disease in humans, domestic and wildlife animals. Dogs are infected by at least six well-characterized Babesia spp. that cause clinical disease. Infection with a piroplasmid species was detected by light microscopy of stained blood smears from five sick dogs from Israel and prompted an investigation on the parasite's identity. Methods: Genetic characterization of the piroplasmid was performed by PCR amplification of the 18S rRNA and the cytochrome c oxidase subunit 1 (cox1) genes, DNA sequencing and phylogenetic analysis. Four of the dogs were co-infected with Borrelia persica (Dschunkowsky, 1913), a relapsing fever spirochete transmitted by the argasid tick Ornithodoros tholozani Laboulbène & Mégnin. Co-infection of dogs with B. persica raised the possibility of transmission by O. tholozani and therefore, a piroplasmid PCR survey of ticks from this species was performed. Results: The infected dogs presented with fever (4/5), anemia, thrombocytopenia (4/5) and icterus (3/5). Comparison of the 18S rRNA and cox1 piroplasmid gene sequences revealed 99-100% identity between sequences amplified from different dogs and ticks. Phylogenetic trees demonstrated a previously undescribed species of Babesia belonging to the western group of Babesia (sensu lato) and closely related to the human pathogen Babesia duncani Conrad, Kjemtrup, Carreno, Thomford, Wainwright, Eberhard, Quick, Telfrom & Herwalt, 2006 while more moderately related to Babesia conradae Kjemtrup, Wainwright, Miller, Penzhorn & Carreno, 2006 which infects dogs. The piroplasm forms detected included tetrads (Maltese cross), merozoite and trophozoite stages whose average size was larger than stages of other canine Babesia spp. belonging to the Babesia (s.l.) and B. gibsoni Patton, 1910, and smaller than other canine Babesia (sensu stricto) spp. Of 212 O. tholozani ticks surveyed, 11 (5.2%) harbored DNA of the new species of Babesia. Conclusions: Babesia negevi n. sp. is described based on morphological and genetic characterization and phylogenetic analyses. The species is named after the Negev desert of southern Israel, where the first infected dog originated from. Despite co-infection in four dogs, the fifth dog had fatal disease attesting that B. negevi n. sp. infection requires clinical attention. Incriminating O. tholozani or another tick species as the vector of Babesia negevi n. sp., would require additional studies.

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“…We detected Bartonella in nearly 4% of hard ticks and spotted-fever-group Rickettsia in 17% of hard ticks collected from domesticated animals in Palestine [24,25]. Similarly, we have found apicomplexan parasites including Theileria, Babesia , and Hepatozoon in ixodid ticks from Palestine [26]. Various pathogens have been detected in ticks collected in Israel, including Ehrlichia canis, Anaplasma spp., Babesia canis vogeli, Rickettsia massiliae, R. sibirica mongolitimonae, R. africae, R. aeschlimannii, [27–31].…”

Section: Introductionmentioning

confidence: 98%

Metagenomic profiling of ticks: identification of novel rickettsial genomes and detection of tick borne canine parvovirus

Ray

Ereqat²,

Al-Jawabreh

et al. 2018

Preprint

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48recovering genome sequences from an unexpected pathogen (canine parvovirus) 49 and a previously unsequenced pathogen (Candidatus Rickettsia barbariae), we50 demonstrate the open-ended nature of metagenomics. We also provide evidence Page 3 of 31 51 that ticks can carry canine parvovirus, raising the possibility that ticks might 52 contribute to the spread of this troublesome virus. 53 Author Summary 54 We have shown how DNA sequencing can be used to detect and characterise 55 potentially pathogenic microorganisms carried by ticks. We surveyed hard ticks 56 collected from domesticated animals across the West Bank territory of Palestine. All 57 the ticks came from species that are also capable of feeding on humans. We 58 detected several important pathogens, including two species of Rickettsia, the sheep 59 pathogen Anaplasma ovis and canine parvovirus. These findings highlight the 60 importance of hard ticks and the hazards they present for human and animal health 61 in Palestine and the opportunities presented by high-throughput sequencing and 62 bioinformatics analyses of DNA sequences in this setting. 63 64

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Molecular detection of Theileria, Babesia, and Hepatozoon spp. in ixodid ticks from Palestine

Cited by 29 publications

References 35 publications

Prevalence, First Molecular Identification and Characterization of Theileria lestoquardi in Sheep in Alhuda National Sheep Research Station, Al Gezira State, Sudan

Prevalence, First Molecular Identification and Characterization of Theileria lestoquardi in Sheep in Alhuda National Sheep Research Station, Al Gezira State, Sudan

A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp.

Metagenomic profiling of ticks: identification of novel rickettsial genomes and detection of tick borne canine parvovirus

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