Molecular detection of “stolbur” phytoplasma in celery plants in Romania

Chireceanu, C.; Chiriloaie, Andrei; Teodoru, Andrei

doi:10.5958/2249-4677.2016.00008.6

Cited by 5 publications

(4 citation statements)

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“…Lee et al (2000) reported phytoplasma of 16SrI-B subgroup infecting celery crops in North America and Europe. Later, other groups of phytoplasmas were reported on celery from other parts of the world, like tomato big bud phytoplasma (16SrII-E) from Australia (Tran-Nguyen et al 2003) and stolbur phytoplasma (16SrXII-A) from Italy, Serbia and Romania (Carraro et al 2008;Ivanović et al 2011;Chireceanu et al 2016), 16SrVII and IX-A groups from New Zealand (Liefting et al 2011) and 16SrI-C from the Czech Republic (Fránová and Š pak 2013). No report of 16SrVI group of phytoplasmas incidence has been recorded earlier on celery from India and abroad, and hence, is the new report.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of ‘Clover proliferation’ phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India

Gopala

Khasa

Rao

et al. 2018

Physiol Mol Biol Plants

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Nine vegetable plants species exhibiting phytoplasma suspected symptoms of white/purple leaf, little leaf, flat stem, witches' broom, phyllody and leaf yellowing were observed in experimental fields at Indian Agricultural Research Institute, New Delhi from December 2015 to July 2016. Total DNA extracted from the three healthy and three symptomatic leaves of all the nine vegetables were subjected to PCR assays using phytoplasma specific primers P1/P7 followed by R16F2n/R16R2 and 3Far/3Rev to amplify the 16S rDNA fragments. No amplifications of DNA were observed in first round PCR assays with primer pair P1/P7 from any of the symptomatic samples. However, phytoplasma DNA specific fragments of ~ 1.3 kb were amplified from L. (two isolates), vr L. (one isolate) and L. (one isolate) by using 3Far/3Rev primer pair and 1.2 kb fragment was amplified from L. (one isolate) by using R16F2n/R16R2 primer pair. No DNA amplification was seen in other symptomatic vegetable samples of tomato, carrot, cucurbit, bitter gourd and species utilizing either P1/P7 primer pair followed by 3Far/3Rev or R16F2n/R16R2 primer pairs. Out of three leafhopper species collected from the symptomatic vegetable fields, only was found positive for association of phytoplasma. No DNA amplifications were observed in healthy plant samples and insects collected from non-symptomatic fields. Comparative sequence comparison analyses of 16S rDNA of positive found vegetable phytoplasma strains revealed 100% sequence identities among each other and with phytoplasma strains of 'clover proliferation' (16SrVI) group. Phytoplasma sequences, virtual RFLPs and phylogenetic analyses of 16S rDNA sequence comparison confirmed the identification of 16SrVI subgroup D strain of phytoplasmas in four vegetables and one leafhopper (HP) species. Further virtual RFLP analysis of 16S rDNA sequence of the vegetables phytoplasma strains confirmed their taxonomic classification with strains of 'clover proliferation' subgroup D. Since, feeding on symptomatic vegetable species in the study was also tested positive for the 16SrVI phytoplasma subgroup-D as of vegetables; it may act as potent natural reservoir of 16SrVI-D subgroup of phytoplasmas infecting vegetable and other important agricultural crops.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of ‘Clover proliferation’ phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India

Gopala

Khasa

Rao

et al. 2018

Physiol Mol Biol Plants

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“…'Ca. P. solani'-infection of celery was so far reported in Hungary, Italy and neighbouring Romania and Bosnia and Herzegovina (Viczián, 2002;Carraro et al, 2008;Chireceanu et al, 2016;Delić et al, 2016). Serious agroeconomic losses of the celery production due to stolbur phytoplasma infection have so far been observed in Italy and correlated to the period of transplanting celery seedling in the open field, reporting 80-90% of infected plants if transplanted at the end of June/first half of July (Carraro et al, 2008).…”

Section: Multilocus Genotyping Of 'Ca P Solani' Strainsmentioning

confidence: 94%

Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

Popović¹,

Mitrović²,

Kosovac³

2021

Ratar i povrt

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'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.

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“…The species is univoltine or bivoltine and the number of generations is different from one country to another and even in a particular country. According to the literature sources the flight of the 1st generation is from the beginning of May till late July-beginning of August, and the 2nd in between mid-August to the second part of September (Chireceanu et al 2020;Nahirnic-Beshkova et al 2021a;Tarmann et al, 2019;Toshova et al 2017). Accorging to some authors univoltine and partially bivoltine populations of Th.…”

Section: Introductionmentioning

confidence: 99%

The northern distribution limit of Theresimima ampellophaga (Bayle-Barelle, 1809) in Albania: results obtained by sex pheromone traps (Lepidoptera: Zygaenidae, Procridinae)

Vrenozi,

Toshova

2024

SHILAP Revta. lepid.

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Theresimima ampellophaga (Bayle-Barelle, 1809) (Lepidoptera: Zygaenidae, Procridinae), occurs as a partly harmful species in vineyards in the Mediterranean region, where it has the proper conditions for larvae development, including the host plants and hilly relief. In 2017, has been registered as a new country record in the vineyards of the western lowland in Albania on the base of a large-scale study using traps baited with the synthetic sex pheromone of Th. ampellophaga females or the sex attractant EFETOV-2. The further field works in 2018 and 2019 using sex pheromone traps aimed to establish in new localities in Albania and determine the northern distribution limit of Th. ampellophaga in this country. In 2019, low numbers males were captured in three vineyards in Apolloni, Radostinë (district of Fier) and Dukat i Ri (district of Vlorë), southern Albania in the first half of July, all from the first generation. As a preliminary result, the northern distribution limit in Albania is reported.

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Molecular detection of “stolbur” phytoplasma in celery plants in Romania

Cited by 5 publications

References 0 publications

Molecular characterization of ‘Clover proliferation’ phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India

Molecular characterization of ‘Clover proliferation’ phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India

Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

The northern distribution limit of Theresimima ampellophaga (Bayle-Barelle, 1809) in Albania: results obtained by sex pheromone traps (Lepidoptera: Zygaenidae, Procridinae)

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