Molecular detection of Rickettsia, Coxiella and Rickettsiella DNA in three native Australian tick species

Vilcins, Inger-Marie E; Old, Julie M.; Deane, Elizabeth M.

doi:10.1007/s10493-009-9260-4

Cited by 48 publications

(48 citation statements)

References 43 publications

(74 reference statements)

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“…This suggests that there was no transfer of Rickettsiella to D. andersoni individuals feeding on the same small mammal host as ticks infected with Rickettsiella. Interestingly, those Rickettsiella detected in ticks thus far have only been found in members of the genus Ixodes: I. ricinus in the Palearctic (34,35), I. woodi in the Nearctic (31) and I. tasmani in Australia (33). The results of the present study also detected Rickettsiella DNA in three species of Ixodes: I. angustus, I. sculptus, and I. kingi.…”

Section: Discussionsupporting

confidence: 59%

“…For example, a Rickettsiella genetically similar to R. grylli has been isolated in the ovarian tissues and malpighian tubules of unfed female Ixodes woodi (31), while female Dermacentor reticulatus have been experimentally infected with "R. phytoseiuli" isolated from the mite Phytoseiulus persimilis (32). Rickettsiella DNA has also been detected by PCR in I. tasmani and I. ricinus (33)(34)(35), and in a few individuals of I. sculptus during a molecular study of the bacterial diversity in this tick species (36). Therefore, the aim of the present study was to develop a PCR-based assay to screen for the presence of Rickettsiella in four species of tick from western Canada and to compare their DNA sequences to those of different species and pathotypes of Rickettsiella.…”

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confidence: 99%

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Discovery of Novel Rickettsiella spp. in Ixodid Ticks from Western Canada

Anstead

Chilton

2014

Appl Environ Microbiol

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The genomic DNA from four species of ixodid ticks in western Canada was tested for the presence of Rickettsiella by PCR analyses targeting the 16S rRNA gene. Eighty-eight percent of the Ixodes angustus (n ‫؍‬ 270), 43% of the I. sculptus (n ‫؍‬ 61), and 4% of the I. kingi (n ‫؍‬ 93) individuals examined were PCR positive for Rickettsiella, whereas there was no evidence for the presence of Rickettsiella in Dermacentor andersoni (n ‫؍‬ 45). Three different single-strand conformation polymorphism profiles of the 16S rRNA gene were detected among amplicons derived from Rickettsiella-positive ticks, each corresponding to a different sequence type. Furthermore, each sequence type was associated with a different tick species. Phylogenetic analyses of sequence data of the 16S rRNA gene and three other genes (rpsA, gidA, and sucB) revealed that all three sequence types were placed in a clade that contained species and pathotypes of the genus Rickettsiella. The bacterium in I. kingi represented the sister taxon to the Rickettsiella in I. sculptus, and both formed a clade with Rickettsiella grylli from crickets (Gryllus bimaculatus) and "R. ixodidis" from I. woodi. In contrast, the Rickettsiella in I. angustus was not a member of this clade but was placed external to the clade comprising the pathotypes of R. popilliae. The results indicate the existence of at least two new species of Rickettsiella: one in I. angustus and another in I. kingi and I. sculptus. However, the Rickettsiella strains in I. kingi and I. sculptus may also represent different species because each had unique sequences for all four genes.

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Section: Discussionsupporting

confidence: 59%

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confidence: 99%

Discovery of Novel Rickettsiella spp. in Ixodid Ticks from Western Canada

Anstead

Chilton

2014

Appl Environ Microbiol

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“…The other 34 I. tasmani individuals sampled, as well as two additional tick species (Bothriocroton concolor and Bothriocroton auruginans), were negative for Rickettsiella. The virulence factors, if any, of Rickettsiella genotypes detected on the tick host remained unknown in this study (42).…”

Section: Discussionmentioning

confidence: 82%

Novel Rickettsiella Bacterium in the Leafhopper Orosius albicinctus (Hemiptera: Cicadellidae)

Iasur-Kruh

Weintraub

Mozes-Daube

et al. 2013

Appl Environ Microbiol

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Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a major vector of phytoplasma diseases in Europe and Asia. Denaturing gradient gel electrophoresis (DGGE) and pyrosequencing were used to survey the main symbionts of O. albicinctus, revealing the obligate symbionts Sulcia and Nasuia, and the facultative symbionts Arsenophonus and Wolbachia, in addition to Rickettsiella. The leafhopper Rickettsiella is allied with bacteria found in ticks. Screening O. albicinctus from the field showed that Rickettsiella is highly prevalent, with over 60% of individuals infected. A stable Rickettsiella infection was maintained in a leafhopper laboratory colony for at least 10 generations, and fluorescence microscopy localized bacteria to accessory glands of the female reproductive tract, suggesting that the bacterium is vertically transmitted. Future studies will be needed to examine how Rickettsiella affects host fitess and its ability to vector phytopathogens.

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“…Ixodid ticks have recently been shown to harbor Coxiella spp. and Coxiella-like pathogens in the wild (Vilcins et al 2009). Due to the efficiency of Ixodes spp.…”

Section: Introductionmentioning

confidence: 99%

Growth ofCoxiella burnetiiin theIxodes scapularis–Derived IDE8 Tick Cell Line

Herrin

Mahapatra²,

Blouin³

et al. 2011

Vector-Borne and Zoonotic Diseases

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Q fever, a zoonotic disease, is caused by a gram-negative intracellular bacterium, Coxiella burnetii. Although normally transmitted during exposure to infectious aerosols, C. burnetii is also found in arthropod vectors. In the environment, ticks are thought to play a crucial role in bacterial maintenance and transmission by infecting various mammalian species. However, the nature of the pathogen-tick relationship is not well defined. To determine C. burnetii's interactions with a cultured tick cell line, we introduced purified C. burnetii NMII into Ixodes scapularisderived IDE8 cells and assayed for bacterial presence, replication, gene expression, and subsequent infectivity for mammalian cells. Tick cells were harvested at 24 h, 72 h, 7 days, and 11 days postinfection (PI). C. burnetii uptake and subsequent replication was demonstrated by indirect immunofluorescence assay, electron microscopy, and real-time polymerase chain reaction (PCR). When a genome equivalent multiplicity of infection of 30 was used, 30%-40% of exposed cells were seen to have small, rounded, vacuoles at 72 h PI, whereas at 7 and 11 days PI, 60%-70% of cells contained enlarged vacuoles harboring large numbers of bacteria. Quantitative PCR analysis of total genomic DNA confirmed that C. burnetii genome numbers increased significantly from 24 h to 11 days PI. Expression of C. burnetii type four secretion system homologs at 7 days PI was demonstrated by reverse transcriptase PCR. Finally, indirect immunofluorescence assay demonstrated that C. burnetii propagated within IDE8 cells were infectious for mammalian cells. These studies demonstrate the utility of cultured tick cell lines as a model to investigate C. burnetii's molecular interactions with its arthropod vectors.

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Molecular detection of Rickettsia, Coxiella and Rickettsiella DNA in three native Australian tick species

Cited by 48 publications

References 43 publications

Discovery of Novel Rickettsiella spp. in Ixodid Ticks from Western Canada

Discovery of Novel Rickettsiella spp. in Ixodid Ticks from Western Canada

Novel Rickettsiella Bacterium in the Leafhopper Orosius albicinctus (Hemiptera: Cicadellidae)

Growth ofCoxiella burnetiiin theIxodes scapularis–Derived IDE8 Tick Cell Line

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