Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis

Rashid, Mamun-Or; Wang, Ying; Han, Chenggui

doi:10.3390/plants9111413

Cited by 12 publications

(5 citation statements)

References 45 publications

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“…In Peru, PVY was shown to be present in ~ 43% of tested samples [ 36 ]. According to a study conducted in Bangladesh’s key potato-growing regions, the prevalence of PLRV, PVX, PVY, PVS, potato virus H (PVH), potato aucuba mosaic virus (PAMV), and PVM ranged from 2 to 8.33% [ 37 ]. PVY, PLRV, and PVM were the most common viruses in India, accounting for 44.26%, 42.62%, and 27.86%, respectively [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring virus presence in field-collected potato leaf samples using RNA sequencing

Elwan,

Rabie,

Aleem

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background The quick and accurate identification of viruses is essential for plant disease management. Next-generation sequencing (NGS) technology may allow the discovery, detection, and identification of plant pathogens. This study adopted RNA-sequencing (RNA-Seq) technology to explore the viruses in three potato plants (S3, S4, and S6) growing under field conditions. Results Potato-known infecting viruses, such as alfalfa mosaic virus (AMV), potato leafroll virus (PLRV), and potato virus Y (PVY), were identified using bioinformatics programs and validated using RT-PCR. The presence of these potato viruses was also confirmed by visual inspection of host symptoms. In addition, the nearly complete genome of PLRV and the complete or partial genome sequence of multipartite virus segments have been identified. Besides the three major potato viruses that BLASTn analysis revealed were present in our samples, BLASTx analysis revealed some reads are derived from other potato viruses, such as potato virus V (PVV), Andean potato latent virus (APLV), and tomato chlorosis virus (ToCV), which are not frequently reported in potato field screenings in Egypt. Other microbial agents, such as bacteria and fungi, were also identified in the examined sample sequences. Some mycovirus sequences derived from ourmia-like viruses and Alternaria alternata chrysovirus were also identified in sample S4, confirming the complexity of the potato microbiome under field conditions. Conclusion NGS quickly and accurately identifies potato plant viruses under field conditions. Implementing this technology on a larger scale is recommended to explore potato fields and imported plants, where symptoms may be absent, unspecific, or only triggered under certain conditions.

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Section: Discussionmentioning

confidence: 99%

Exploring virus presence in field-collected potato leaf samples using RNA sequencing

Elwan,

Rabie,

Aleem

et al. 2023

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Across the sector, the production of potato, pumpkin, brinjal, cucumber, cabbage, beans, tomato, radish, cauliflower, and many more are continuing to increase. Throughout 2018, Bangladesh produces ∼9,744,412 tonnes of potato, with a world sixth, out of approximately ∼477,419 ha of potato [ 69 ].…”

Section: Effective Geothermal Energy Management Modelmentioning

confidence: 99%

Trends and prospects of geothermal energy as an alternative source of power: A comprehensive review

Islam

Nabi

Arefin

et al. 2022

Heliyon

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“…Viruses are one of the most signi cant biotic constraints in global potato production, affecting both tuber yield and quality (9,10]. More than 50 viruses and one viroid have been identi ed as infecting potatoes around the world, causing varying degrees of loss depending on virus type, strain, seed system, and crop production practices [9].…”

Section: Introductionmentioning

confidence: 99%

Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

Tessema

Kakuhenzire

Seid

et al. 2023

Preprint

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Viruses are among the significant biotic constraints burdening global potato production, particularly in developing countries where seed certification is not common and production, availability and accessibility to quality seed potato is a big hindrance. Virus infection in seed potato reduces yield, and the problem is exacerbated when an early-generation seed is affected. The prevalence of six key potato viruses, PVY, PVX, PLRV, PVA, PVS, and PVM, was assessed among decentralized seed multipliers such as individuals, farmer seed group cooperatives, private companies, and agricultural research center that produce early generation seed in six major potato growing districts in Ethiopia. A total of 262 leaf samples were randomly collected from potato plants and analyzed using a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for potato viruses. Potato virus prevalence was calculated as the proportion of samples that tested positive for the viruses against the total number of samples tested. Only 1.8% of the samples were virus-free, while 98.2% of the samples collected from farmers' fields were infected with at least one of the six viruses. About 17% of the samples had a single viral infection with one of the six viruses, whereas 81% had multiple infections. Early generation seed and germplasm samples collected from breeding, research, and private screenhouses were infected with PLRV (68.1%), PVY (57.4%), PVS (40.4%), PVM (34%) and PVX (23.4%). These results indicate that virus infection is widespread in the country, posing challenges and threats potato production and food security efforts. To address this issue, it is critical to developing a robust system that prevents viral infection build-up and spread in the seed system through regular seed quality assurance and certification, particularly for early generation seed. Furthermore, smallholder farmers need to use an integrated management approach to effectively combat potato viruses.

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Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis

Cited by 12 publications

References 45 publications

Exploring virus presence in field-collected potato leaf samples using RNA sequencing

Exploring virus presence in field-collected potato leaf samples using RNA sequencing

Trends and prospects of geothermal energy as an alternative source of power: A comprehensive review

Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

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