Molecular detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues and biopsies of gastrointestinal specimens using real-time polymerase chain reaction system

Mycobacterium tuberculosis complex: Detection and patterns of resistance to the first line anti-TB drugs at the King Abdulaziz University Hospital, Saudi Arabia

“…Our results were less than those of Ioannidis et al [14] and Mishra et al [15] who reported that the sensitivity of PCR for MTBC detection of AFB-positive samples were 100% and 96.4%, respectively. Our findings regarding PCR detection of MTBC in AFB-negative samples did not correlate well with those reported by others [16,17], since their studies were performed mainly on respiratory samples.…”

Fluorescent quantitative PCR detection ofMycobacterium tuberculosisin tissue sections from granulomatous lesions retrieved using EDTA

“…The detection rate of tuberculous mycobacteria using fluorescent quantitative PCR in frozen tissue sections of TB lesions was up to 75.8% 6–8. However, the tuberculous mycobacteria detection rate was just 50%–60% when the same method was used in the tissue sections of granulomatous lesions that were formalin fixed and paraffin embedded 3. Sometimes tuberculous mycobacteria could not be detected in paraffin-embedded specimens of typical TB in our work.…”

Comparison of the Diagnostic Performance of Two Real-Time PCR Systems for the Detection of Mycobacterium tuberculosis in Clinical Samples

“…Floresan enerji transfer probları kullanılarak gerçekleştirilen PCR işleminin, gastrointestinal sistemden alınmış formalin ile fikse edilmiş ve parafine gömülmüş doku örneklerinde MTB DNA'sının tespit edilmesinde oldukça duyarlı olduğu; PPD'nin %100, NPD'nin %96.9 olarak bulunduğu bildirilmiştir (16) . Hur ve ark.…”