Molecular Detection of Multidrug Resistance Pathogenic Bacteria from Protective Materials Used By Healthcare Workers (HCW); Bangladesh Scenario

Sultana, Afroza; Saha, Otun; Sid, Ashfaqur Rahman; Saha, Apurbha; Hussain, Md. Saddam; Islam, Tarequl

doi:10.3923/jas.2018.48.55

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…RAPD-PCR was performed using the extracted DNA to investigate the biotype diversity of P. multocida in the study isolates [33,34]. The RAPD-PCR was performed using (5´-GCGATCCCCA-3´) primer following the previously optimized protocol for Escherichia coli [22].…”

Section: Molecular Typing and Detection Of Pathogenic Genesmentioning

confidence: 99%

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

et al. 2021

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Background and Aim: Fowl cholera (FC) caused by Pasteurella multocida is a highly contagious bacterial disease of global importance for poultry production. The severity and incidence of FC caused by P. multocida may vary considerably depending on several factors associated with the host (including species and age of infected birds), the environment, and the bacterial strain. This study aimed to investigate the genetic diversity of multidrug-resistant P. multocida strains isolated from FC outbreaks in laying hens from commercial farms of Bangladesh. Materials and Methods: We collected 57 samples of suspected FC, including 36 live and 21 dead laying hens. P. multocida isolates were characterized by biochemical and molecular-biological methods. Results: Twenty-two strains of P. multocida were isolated from these samples through phenotypic and genotypic characterization. The strains were grouped into two distinct random amplification of polymorphic DNA (RAPD) biotypes harboring a range of pathogenic genes; exbB, ompH, ptfA, nanB, sodC, and hgbA. In this study, 90.90% and 81.82% P. multocida strains were multidrug-resistant and biofilm formers, respectively. Whole-genome sequencing of the two representative RAPD phylotypes confirmed as P. multocida type B: L2:ST122, harboring a number of virulence factors-associated genes (VFGs), and antimicrobial resistance (AMR) genes (ARGs). In addition, pan-genome analysis revealed 90 unique genes in the genomes of P. multocida predicted to be associated with versatile metabolic functions, pathogenicity, virulence, and AMR. Conclusion: This is first-ever report on the association of P. multocida genotype B: L2:ST122 and related VFGs and ARGs in the pathogenesis of FC in laying hens. This study also provides a genetic context for future researches on the evolutionary diversity of P. multocida strains and their host adaptation.

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Section: Molecular Typing and Detection Of Pathogenic Genesmentioning

confidence: 99%

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

et al. 2021

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“…The global health problem of antibiotic resistance (AR) is exacerbated by the movement of micro-organisms and genes between people, animals, instruments, and the environment [ 111 , 112 , 113 , 114 ]. Despite numerous barriers prohibiting both bacteria and genes from moving freely, pathogens frequently acquire new resistance components from other species, making it more difficult to prevent and treat bacterial illnesses.…”

Section: Plant Bacterial Pathogens and Their Control By Antibioticsmentioning

confidence: 99%

“…Bacterial pathogens are known for their genetic plasticity, which can cause mutational modifications, transfer of genetic material, or changes in gene expression, resulting in resistance to nearly all antibiotics used in clinical practice [ 4 , 132 ]. Acquisition of resistance plasmids or chromosomal mutations are the two primary mechanisms involved in AR [ 113 , 114 ]. Plasmids bearing resistance genes can result in antibiotic degradation through enzyme synthesis or enzymatic modification, while mutations leading to antimicrobial resistance can alter antibiotic activity by modifying the antibiotic target site, activating harmful molecule excretion, reducing drug uptake, or changing important metabolic pathways [ 4 ].…”

Section: Mechanisms Of Antibiotic Resistancementioning

confidence: 99%

Antibiotic Resistance in Plant Pathogenic Bacteria: Recent Data and Environmental Impact of Unchecked Use and the Potential of Biocontrol Agents as an Eco-Friendly Alternative

Islam,

Haque,

Barai

et al. 2024

Plants

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The economic impact of phytopathogenic bacteria on agriculture is staggering, costing billions of US dollars globally. Pseudomonas syringae is the top most phytopathogenic bacteria, having more than 60 pathovars, which cause bacteria speck in tomatoes, halo blight in beans, and so on. Although antibiotics or a combination of antibiotics are used to manage infectious diseases in plants, they are employed far less in agriculture compared to human and animal populations. Moreover, the majority of antibiotics used in plants are immediately washed away, leading to environmental damage to ecosystems and food chains. Due to the serious risk of antibiotic resistance (AR) and the potential for environmental contamination with antibiotic residues and resistance genes, the use of unchecked antibiotics against phytopathogenic bacteria is not advisable. Despite the significant concern regarding AR in the world today, there are inadequate and outdated data on the AR of phytopathogenic bacteria. This review presents recent AR data on plant pathogenic bacteria (PPB), along with their environmental impact. In light of these findings, we suggest the use of biocontrol agents as a sustainable, eco-friendly, and effective alternative to controlling phytopathogenic bacteria.

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“…Genomic DNA of E. coli was extracted from overnight culture by the boiled DNA extraction method [50]. Briefly, the samples were centrifuged at 15,000× g for 15 min [50].…”

Section: Dna Extractionmentioning

confidence: 99%

“…Genomic DNA of E. coli was extracted from overnight culture by the boiled DNA extraction method [50]. Briefly, the samples were centrifuged at 15,000× g for 15 min [50]. We eliminated the supernatant, resuspended the pellets in filtered (22 microliter) distilled water, and centrifuged at 15,000× g for 10 min.…”

Section: Dna Extractionmentioning

confidence: 99%

Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

et al. 2020

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The avian pathogenic Escherichia coli (APEC) strains are the chief etiology of colibacillosis worldwide. The present study investigated the circulating phylotypes, existence of virulence genes (VGs), and antimicrobial resistance (AMR) in 392 APEC isolates, obtained from 130 samples belonged to six farms using both phenotypic and PCR-based molecular approaches. Congo red binding (CRB) assay confirmed 174 APEC isolates which were segregated into ten, nine, and eight distinct genotypes by RAPD assay (discriminatory index, DI = 0.8707), BOX-PCR (DI = 0.8591) and ERIC-PCR (DI = 0.8371), respectively. The combination of three phylogenetic markers (chuA, yjaA and DNA fragment TspE4.C2) classified APEC isolates into B23 (37.36%), A1 (33.91%), D2 (11.49%), B22 (9.20%), and B1 (8.05%) phylotypes. Majority of the APEC isolates (75–100%) harbored VGs (ial, fimH, crl, papC, and cjrC). These VGs (papC and cjrC) and phylotypes (D2 and B2) of APEC had significant (p = 0.004) association with colibacillosis. Phylogenetic analysis showed two distinct clades (clade A and clade B) of APEC, where clade A had 98–100% similarity with E. coli APEC O78 and E. coli EHEC strains, and clade B had closest relationship with E. coli O169:H41 strain. Interestingly, phylogroups B2 and D2 were found in the APEC strains of both clades, while the strains from phylogroups A1 and B1 were found in clade A only. In this study, 81.71% of the isolates were biofilm formers, and possessed plasmids of varying ranges (1.0 to 54 kb). In vitro antibiogram profiling revealed that 100% isolates were resistant to ≥3 antibiotics, of which 61.96%, 55.24%, 53.85%, 51.16% and 45.58% isolates in phylotypes B1, D2, B22, B23, and A1, respectively, were resistant to these antimicrobials. The resistance patterns varied among different phylotypes, notably in phylotype B22, showing the highest resistance to ampicillin (90.91%), nalidixic acid (90.11%), tetracycline (83.72%), and nitrofurantoin (65.12%). Correspondence analysis also showed significant correlation among phylotypes with CRB (p = 0.008), biofilm formation (p = 0.02), drug resistance (p = 0.03), and VGs (p = 0.06). This report demonstrated that B2 and A1 phylotypes are dominantly circulating APEC phylotypes in Bangladesh; however, B2 and D2 are strongly associated with the pathogenicity. A high prevalence of antibiotic-resistant APEC strains from different phylotypes suggest the use of organic antimicrobial compounds, and/or metals, and the rotational use of antibiotics in poultry farms in Bangladesh.

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Molecular Detection of Multidrug Resistance Pathogenic Bacteria from Protective Materials Used By Healthcare Workers (HCW); Bangladesh Scenario

Cited by 6 publications

References 19 publications

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

Antibiotic Resistance in Plant Pathogenic Bacteria: Recent Data and Environmental Impact of Unchecked Use and the Potential of Biocontrol Agents as an Eco-Friendly Alternative

Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

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