Molecular detection of Rickettsia aeschlimannii in Hyalomma spp. ticks from camels (Camelus dromedarius) in Nigeria, West Africa

Kamani, Joshua; Baneth, Gad; Apanaskevich, Dmitry A.; Mumcuoglu, Kosta Y.; Harrus, Shimon

doi:10.1111/mve.12094

Cited by 37 publications

(41 citation statements)

References 20 publications

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“…Regarding camels, this prevalence is similar to that recorded in Palestine (2%) (Kleinerman et al, ). Contrariwise, this result is significantly lower than those reported earlier in Nigeria (18.8%) (Kamani et al, ) and in Egypt (41%) (Abdullah et al, ).…”

Section: Discussioncontrasting

confidence: 68%

“…Camelids seem to be potential hosts of rickettsial species, particularly in African and Asian arid regions. Molecular prevalence in camels was estimated at 41% in Egypt (Abdullah et al, ) and 18.8% in Nigeria (Kamani, Baneth, Apanaskevich, Mumcuoglu, & Harrus, ). Rickettsia aeschlimannii was identified in camels from Palestine (2%) (Kleinerman, Baneth, Mumcuoglu, Straten, & v., Berlin, D., Apanaskevich, D. A., Abdeen, Z., Nasereddin, A.…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

Selmi

Saïd

Yahia

et al. 2019

Transbound Emerg Dis

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Rickettsia species are adapted to a wide range of specific animal hosts. Camels (Camelus dromedarius) have been identified as a carrier of various zoonotic pathogens and became a focus of growing public health interest. This study reported the occurrence of rickettsial infection in camels and infesting ticks from five Tunisian governorates. Based on ompB PCR, eight out of 293 camels (2.7%) were found to be infected with Rickettsia spp. Furthermore, 13 tick specimens of Hyalomma impeltatum (10.4%) and 9 of H. dromedarii (8.0%) harboured DNA of Rickettsia bacteria with an overall prevalence rate of 9.2% (22/237). Molecular prevalence of Rickettsia infection varied significantly according to tick infestation for camels and among tick genders. Five rickettsial species, showing a potential public health interest, were revealed by sequencing. Based on ompB partial sequences, five species were identified corresponding to R. aeschlimannii, R. monacensis, R. helvetica and R. massiliae in camels and to R. africae, R. aeschlimannii, R. monacensis and R. helvetica in ticks. Based on ompA typing, three species were revealed corresponding to R. africae and R. monacensis in camels and to R. africae, R. aeschlimannii and R. monacensis in ticks. This is the first report consolidating the hypothesis that camels may serve as potential hosts for Rickettsia spp. and Hyalomma spp. ticks as possible vectors in arid and Saharan areas of Tunisia. The present data highlight the importance of preventive measures and survey that must be implemented in camel herds in order to limit the spread of these vector‐borne bacteria to animals and humans.

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Section: Discussioncontrasting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

Selmi

Saïd

Yahia

et al. 2019

Transbound Emerg Dis

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“…have been detected in camel blood serum with prevalences of up to 83% [21,58] and R. aeschlimannii, R. africae, R. sibirica mongolitimonae and Rickettsia sp. have been identified in several tick species collected from camels [20,[59][60][61][62][63][64][65][66]. Despite this, there are no reports on diseases in camels caused by these organisms.…”

Section: Discussionmentioning

confidence: 99%

Vector-borne bacteria in blood of camels in Iran: New data and literature review

Sazmand

Harl

Eigner

et al. 2019

Comparative Immunology, Microbiology and Infectious Diseases

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A B S T R A C TDespite close association between camels and humans, molecular based studies on vector-borne pathogens infecting camels are scarce compared to other animals in Iran. The current study was carried out to investigate the occurrence of vector-borne bacteria in the blood of dromedaries by molecular tools. A total of 200 peripheral blood samples were collected from apparently healthy animals. Microscopic examination was performed on Giemsa-stained blood smears, and drops of blood were spotted on Whatman FTA ® cards for molecular analyses. Genomic DNA was extracted from the cards, and PCR amplification followed by sequencing of positive samples was carried out for the detection of Anaplasmataceae, spotted fever group (SFG) rickettsiae, Bartonella spp. and Borrelia spp. Intra-cytic forms of any blood pathogens could not be detected by light microscopy. PCR results revealed 30 animals (15%) to be infected with Anaplasmataceae bacteria. Analyses of sequences revealed a strain of Anaplasma sp. identical to Candidatus Anaplasma camelii isolated from camels, cattle and deer in Asia and Africa. Neither SFG rickettsiae, nor Borrelia or Bartonella species were found. Further studies for determining epidemiological role of camels and its zoonotic potential are recommended. This paper reviews the current knowledge on camels' tickborne bacteria including microscopy, serology and molecular studies.confirmed by DNA sequencing in camels are Candidatus Anaplasma camelii (genetically close to A. platys), A. phagocytophilum and A. ovis [10][11][12][13][14][15][16][17][18]. Clinical signs of natural infections are described as fever, pale and icteric conjunctiva suggestive of anaemia, dullness, anorexia, diarrhoea, loss of appetite, emaciation, coughing, lacrimation, rough hair coat, abortion, and/or infertility [7,19]. Intramuscular administration of oxytetracycline at 20 mg/kg as specific therapy and injectable B-complex, iron, folic acid and hydroxycobalamin as supportive therapy is the recommended therapeutic regimen [7,19]. Infection with and antibodies against several species of Rickettsia [20,21], as well as infection with Bartonella [22] and Borrelia [23,24] have been also documented.According to official estimates, around 183,900 camels live in Iran [25]. Given the growing scientific and public health interest in camels, we investigated the occurrence of selected vector-borne bacteria;https://doi.

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“…In the previous studies, Rickettsia spp. were identified in camel blood film stain from Dubai (Wernery et al, 2001) and 18.8% of camel blood samples by PCR from Nigeria (Kamani et al, 2015). On the other hand, Mentaberre et al (2013) reported that 83 % of camels were found infected with Rickettsia spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diagnosis of Rickettsiae Infecting Camels and Ixodid Ticks in Egypt

Abdullah¹,

El-Molla²,

Salib³

et al. 2019

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Rickettsioses including their pathogens, vectors, and hosts have an epidemiological importance and zoonotic importance. The objective of the present article was to define the prevalence and genotypic properties of Rickettsia in camels and their ticks in Egypt. Sixty one blood samples and 99 adult ticks were taken from camel hosts from Cairo, Giza and Sinai, during a period extending from 2013 to 2014. Based on the morphological identification of both male and female tick specimens, 91.9 % of the collected ticks were Hyalomma dromedarii. The prevalence of Rickettsia in camels using Gimenez staining technique and PCR was 0 and 41 %, respectively. The rickettsiae infection in ticks recorded 10.1 and 1.01 %, by Gimenez stain and PCR, respectively. Further, the phylogenetic analysis was conducted based on the sequences of OmpA and gltAgenes and three intergenic spacers (mppA, dksA and rpmE) of Rickettsia species. The phylogenetic analyses revealed a novel strain of Rickettsia africae in Hyalomma marginatum that was collected from camel in Sinai province. In addition, the phylogenetic analysis based on Clustal omega suggested that Rickettsia sequences which detected in camels were R. africae. Moreover, the highest Rickettsial infection rate was recorded in age groups of 17 to 19 years (80.0 %), Abady camel breeds (56.8 %) and ticks-infested camels (42.8 %). Concerning hematological changes, macrocytic anemia and leucopenia were recorded in camels with rickettsioses. The molecular characterization of Rickettsia detected in camels and their tick vectors will help in a better understanding of the epidemiological approach of rickettsioses in Egypt.

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Molecular detection of Rickettsia aeschlimannii in Hyalomma spp. ticks from camels (Camelus dromedarius) in Nigeria, West Africa

Cited by 37 publications

References 20 publications

Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

Vector-borne bacteria in blood of camels in Iran: New data and literature review

Molecular Diagnosis of Rickettsiae Infecting Camels and Ixodid Ticks in Egypt

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