Molecular Detection of
            <i>Mycoplasma pneumoniae</i>
            in Adults with Community-Acquired Pneumonia Requiring Hospitalization

Dorigo-Zetsma, J. W.; Verkooyen, R. P.; Helden, H. P. Van; Nat, Hans van der; Bosch, J.L.H. Ruud

doi:10.1128/jcm.39.3.1184-1186.2001

Cited by 63 publications

(58 citation statements)

References 15 publications

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“…Räty et al (79) collected sputum, an NPA and a OPS specimen from 32 young military conscripts with pneumonia during an M. pneumoniae outbreak and applied PCR and also concluded that sputum was the best sample to detect M. pneumoniae. Dorigo-Zetsma et al also found that sputum was the specimen that was most likely to be PCR positive (62.5%, versus 41% for nasopharynx, 28% for OPSs, and 44% for throat washes (31). The superiority of sputum in these studies was in agreement with earlier studies when M. pneumoniae was detected by probe hybridization from sputum and OPSs (57) and by antigen detection from sputum and NPAs (56).…”

Section: Gnarpe Et Al (43) Compared Npss Andsupporting

confidence: 82%

Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections

et al. 2009

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Section: Gnarpe Et Al (43) Compared Npss Andsupporting

confidence: 82%

Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections

et al. 2009

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“…As presented in this study, definite diagnosis remains dependent on serological tests, which may require several weeks, and the culture of mycoplasma is not practical. Although current rapid diagnostic tools using PCR, which has high specificity and sensitivity, are available [14,15], they may not be applicable in every medical institute. Thus, the development of newer diagnostic tools is warranted.…”

Section: Discussionmentioning

confidence: 99%

Clinical features, risk factors and treatment of fulminant Mycoplasma pneumoniae pneumonia: A review of the Japanese literature

Izumikawa

Takazono

et al. 2014

Journal of Infection and Chemotherapy

100

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Mycoplasma pneumoniae (MP) is one of the most common causes of community-acquired pneumonia in children and young adults. Although MP sometimes causes self-limiting pneumonia, severe and fulminant cases with hypoxia occur, but their clinical features have rarely been reported. This study aimed to reveal the clinical manifestations, risk factors, and treatment of fulminant MP pneumonia (MPP). Using PubMed and abstracts from the proceedings of several domestic Japanese academic societies, we reviewed the Japanese and English literature for cases of fulminant or severe MPP reported in Japan. All clinical information such as sex, age, underlying diseases, clinical symptoms, clinical course, laboratory and radiological findings, and treatment was collected and analyzed. In total, 52 fulminant MPP cases were reported between September, 1979 and February, 2010. The dominant population of fulminant MPP was young adults without severe underlying diseases. Cough (97.3%), fever (100.0%), and dyspnea (83.3%) with diffuse abnormal findings in radiological examinations were noted. Antibiotics without anti-mycoplasmal activity were used in 32 cases (61.5%) as initial treatment prior to the onset of hypoxia. Anti-mycoplasmal drugs were appropriately used in 41 cases (78.8%) after onset of respiratory failure with steroids (23 cases, 45.1%) and effective. The majority of patients improved within 3-5 3 days after steroid administration. There were only 2 fatal cases. Although this small retrospective study did not reveal the apparent risk factors of fulminant MPP, initial inappropriate use of antibiotics may be a risk factor, and early administration of appropriate anti-mycoplasmal drugs with steroids as a cellular immune suppressor is required.4

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“…applied to bronchoalveolar lavage (BAL) fluid has been found useful [8], PCR for Mycoplasma pneumoniae and Chlamydophila pneumoniae has not been evaluated in BAL samples. However, as sputum samples have been shown to be more useful than upper respiratory tract samples for PCR identification of M. pneumoniae [9,10] and C. pneumoniae [11], and because sputum samples can often not be collected in patients with LRTI, BAL PCR could probably be useful for identification of these two pathogens in LRTI patients.…”

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confidence: 99%

Evaluation of a multiplex PCR for bacterial pathogens applied to bronchoalveolar lavage

Strålin

Korsgaard²,

Olcén³

2006

European Respiratory Journal

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The present study assessed the diagnostic usefulness of a multiplex PCR (mPCR) for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae applied to bronchoalveolar lavage (BAL).Fibreoptic bronchoscopy was performed on 156 hospitalised adult patients with lower respiratory tract infection (LRTI) and 36 controls. BAL fluid was analysed with bacterial culture and mPCR.By conventional diagnostic methods, S. pneumoniae, H. influenzae, M. pneumoniae and C. pneumoniae were aetiological agents in 14, 21, 3.2 and 0% of the LRTI patients, respectively. These pathogens were identified by BAL mPCR in 28, 47, 3.2 and 0.6% of cases, respectively, yielding sensitivities of 86% for S. pneumoniae, 88% for H. influenzae, 100% for M. pneumoniae and 0% for C. pneumoniae, and specificities of 81, 64, 100 and 99% for S. pneumoniae, H. influenzae, M. pneumoniae and C. pneumoniae, respectively. Of the 103 patients who had taken antibiotics prior to bronchoscopy, S. pneumoniae was identified by culture in 2.9% and by mPCR in 31%. Among the controls, mPCR identified S. pneumoniae in 11% and H. influenzae in 39%.In lower respiratory tract infection patients, bronchoalveolar lavage multiplex PCR can be useful for identification of Streptococcus pneumoniae, Mycoplasma pneumoniae and Chlamydophila pneumoniae. The method appears to be particularly useful in patients treated with antibiotics.

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Molecular Detection of Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia Requiring Hospitalization

Cited by 63 publications

References 15 publications

Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections

Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections

Clinical features, risk factors and treatment of fulminant Mycoplasma pneumoniae pneumonia: A review of the Japanese literature

Evaluation of a multiplex PCR for bacterial pathogens applied to bronchoalveolar lavage

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