Molecular Detection of <i>Leishmania infantum</i> in Wild Rodents (<i>Rattus norvegicus</i>) in Greece

Papadogiannakis, E.; Spanakos, Gregory; Kontos, V.; Menounos, Panagiotis; Τέγος, Νικόλαος; Vakalis, Nicholas

doi:10.1111/j.1863-2378.2009.01264.x

Cited by 41 publications

(27 citation statements)

References 8 publications

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“…Neitzke-Abreu et al (2013) tested the accuracy of some PCR-based techniques for CL diagnosis in humans and concluded that the use of blood (buffy coat) may be indicated for patients with no lesions. Our prevalence was higher than reported in other Brazilian (CÁSSIA-PIRES et al, 2014;LARA-SILVA et al, 2014) and foreign studies (LIMA et al, 2002;PAPADOGIANNAKIS et al, 2010;MENDONÇA et al, 2011;SHENDER et al, 2014;VLADIMIR et al, 2015). One study analyzed rodents from waste sites (VLADIMIR et al, 2015) in Croatia; 1/173 (0.5%) rodent (R. rattus) tested positive for L. (L.) infantum; the authors identified five species of phlebotomine sand flies at those places.…”

Section: Discussioncontrasting

confidence: 51%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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Leishmania in synanthropic rodents (Rattus rattus):new evidence for the urbanization of Leishmania (Leishmania) amazonensis AbstractThis study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate. The positive samples were sequenced twice by Sanger method to species determination. In total, 181 rodents were captured, all were identified as Rattus rattus and none showed clinical alterations. Forty-one of the 176 (23.3%) animals were positive for Leishmania by ELISA and 6/181 (3.3%) were positive by IFAT. Nine of 127 tested animals (7.1%) were positive by PCR; seven were identified as L.

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Section: Discussioncontrasting

confidence: 51%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…were reported [15]. In Italy, L. infantum , was identified [16], as well as in Cyprus [17] and Greece [18]. In Iran, L .…”

Section: Introductionmentioning

confidence: 99%

Natural infection of synathropic rodent species Mus musculus and Rattus norvegicus by Leishmania infantum in Sesimbra and Sintra – Portugal

Helhazar¹,

Leitão²,

Duarte³

et al. 2013

Parasites Vectors

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BackgroundCanine leishmaniosis caused by Leishmania infantum is a parasitic zoonotic disease transmitted by phlebotomine sandflies (Diptera: Psychodidae). Genus Phlebotomus is the biological vector in the Old World and Lutzomyia in the New World. The dog is the domestic reservoir host but other animals like the fox (Vulpes vulpes) and rodents are known to maintain the infection in both sylvatic and domestic cycles.MethodsTo identify the role of synanthropic rodents Mus musculus and Rattus norvegicus as reservoir hosts for Leishmania infantum natural infection, 30 rodents were captured under a trap rodent control program in two private dog shelters from Sintra and Sesimbra, located in the Lisbon Metropolitan Area, known to be endemic for canine leishmaniosis in Portugal. Tissue samples were screened for the presence of Leishmania amastigotes by qPCR and parasitological analysis.ResultsA total of 33.3% (9/27) of Mus musculus rodents revealed the presence of Leishmania spp. DNA while 29.6% (8/27) were positive in the parasitological analysis. Concerning Rattus norvegicus (n=3), one animal revealed infection only by parasitological analysis.ConclusionsOur results identified for the first time in Portugal the presence of Leishmania infection in both rodent species. As susceptible hosts, infected Mus musculus and Rattus norvegicus may increase the risk for dog and human infection in households and surrounding areas, enhancing the need for efficient rodent control measures in shelters and risk zones to prevent transmission of the infection.

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“…Leishmania sp. was also detected by PCR in three rodent species, Rattus rattus, Thrichomys apereoides, and Oryzomys subflavus in Brazil (Oliveira et al 2005), and Rattus norvegicus in Greece (Papadogiannakis et al 2010). In Spain, Lepus granatensis were reported to be infected with L. infantum, and they were capable of infecting sandflies as confirmed by xenodiagnosis (Molina et al 2012).…”

Section: Discussionmentioning

confidence: 97%

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

et al. 2014

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Pesq. Vet. Bras. 34(12):1243-1246, dezembro 2014 1243 RESUMO.-[Diagnóstico de infecção por Leishmania infantum pela reação em cadeia da polimerase em mamíferos silvestres.] A leishmaniose visceral é uma doença infecciosa crônica de mamíferos causada, no Brasil, pelo protozoário Leishmania infantum (sinonímia: Leishmania chagasi) e transmitida pelo flebótomo Lutzomyia longipalpis. Trata-se de uma zoonose endêmica em muitas regiões do Brasil, inclusive em Belo Horizonte, Minas Gerais. Em centros urbanos, leishmaniose visceral acomete principalmente o cão doméstico. Entretanto, L. infantum já foi diagnosticada em outras espécies, incluindo canídeos e primatas de cativeiro em zoológicos. Este estudo buscou avaliar a presença do DNA deste agente em animais de cativeiro e de vida livre da Fundação Zoobotânica de Belo Horizonte através da reação em cadeia da polimerase. Foram analisadas oitenta e uma amostras de sangue oriundas de primatas, carnívoros, ruminantes, edentatos, marsupial e herbívoro de estômago simples. Três primatas Alouatta guariba (bugio marrom) e dois canídeos Speothos venaticus (cachorro--do-mato-vinagre), foram positivos, demonstrando a importância do controle da leishmaniose em áreas endêmicas com a finalidade de conservar a fauna silvestre mantida em cativeiro.

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Molecular Detection of Leishmania infantum in Wild Rodents (Rattus norvegicus) in Greece

Cited by 41 publications

References 8 publications

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Natural infection of synathropic rodent species Mus musculus and Rattus norvegicus by Leishmania infantum in Sesimbra and Sintra – Portugal

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

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